Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (<it>Puccinia striiformis </it>f. sp. <it>tritici</it>)
<p>Abstract</p> <p>Background</p> <p><it>Puccinia striiformis </it>is a plant pathogenic fungus causing stripe rust, one of the most important diseases on cereal crops and grasses worldwide. However, little is know about its genome and genes involved in the...
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| Format: | Article |
| Language: | English |
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BMC
2007-06-01
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| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/8/145 |
| _version_ | 1818667350775300096 |
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| author | Chen Xianming Wang Meinan Ling Peng Campbell Kimberly |
| author_facet | Chen Xianming Wang Meinan Ling Peng Campbell Kimberly |
| author_sort | Chen Xianming |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Puccinia striiformis </it>is a plant pathogenic fungus causing stripe rust, one of the most important diseases on cereal crops and grasses worldwide. However, little is know about its genome and genes involved in the biology and pathogenicity of the pathogen. We initiated the functional genomic research of the fungus by constructing a full-length cDNA and determined functions of the first group of genes by sequence comparison of cDNA clones to genes reported in other fungi.</p> <p>Results</p> <p>A full-length cDNA library, consisting of 42,240 clones with an average cDNA insert of 1.9 kb, was constructed using urediniospores of race PST-78 of <it>P. striiformis </it>f. sp. <it>tritici</it>. From 196 sequenced cDNA clones, we determined functions of 73 clones (37.2%). In addition, 36 clones (18.4%) had significant homology to hypothetical proteins, 37 clones (18.9%) had some homology to genes in other fungi, and the remaining 50 clones (25.5%) did not produce any hits. From the 73 clones with functions, we identified 51 different genes encoding protein products that are involved in amino acid metabolism, cell defense, cell cycle, cell signaling, cell structure and growth, energy cycle, lipid and nucleotide metabolism, protein modification, ribosomal protein complex, sugar metabolism, transcription factor, transport metabolism, and virulence/infection.</p> <p>Conclusion</p> <p>The full-length cDNA library is useful in identifying functional genes of <it>P. striiformis</it>.</p> |
| first_indexed | 2024-12-17T06:19:02Z |
| format | Article |
| id | doaj.art-2053e784b38f4e75b1c1f2b2b48e184d |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-12-17T06:19:02Z |
| publishDate | 2007-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-2053e784b38f4e75b1c1f2b2b48e184d2022-12-21T22:00:26ZengBMCBMC Genomics1471-21642007-06-018114510.1186/1471-2164-8-145Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (<it>Puccinia striiformis </it>f. sp. <it>tritici</it>)Chen XianmingWang MeinanLing PengCampbell Kimberly<p>Abstract</p> <p>Background</p> <p><it>Puccinia striiformis </it>is a plant pathogenic fungus causing stripe rust, one of the most important diseases on cereal crops and grasses worldwide. However, little is know about its genome and genes involved in the biology and pathogenicity of the pathogen. We initiated the functional genomic research of the fungus by constructing a full-length cDNA and determined functions of the first group of genes by sequence comparison of cDNA clones to genes reported in other fungi.</p> <p>Results</p> <p>A full-length cDNA library, consisting of 42,240 clones with an average cDNA insert of 1.9 kb, was constructed using urediniospores of race PST-78 of <it>P. striiformis </it>f. sp. <it>tritici</it>. From 196 sequenced cDNA clones, we determined functions of 73 clones (37.2%). In addition, 36 clones (18.4%) had significant homology to hypothetical proteins, 37 clones (18.9%) had some homology to genes in other fungi, and the remaining 50 clones (25.5%) did not produce any hits. From the 73 clones with functions, we identified 51 different genes encoding protein products that are involved in amino acid metabolism, cell defense, cell cycle, cell signaling, cell structure and growth, energy cycle, lipid and nucleotide metabolism, protein modification, ribosomal protein complex, sugar metabolism, transcription factor, transport metabolism, and virulence/infection.</p> <p>Conclusion</p> <p>The full-length cDNA library is useful in identifying functional genes of <it>P. striiformis</it>.</p>http://www.biomedcentral.com/1471-2164/8/145 |
| spellingShingle | Chen Xianming Wang Meinan Ling Peng Campbell Kimberly Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (<it>Puccinia striiformis </it>f. sp. <it>tritici</it>) BMC Genomics |
| title | Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (<it>Puccinia striiformis </it>f. sp. <it>tritici</it>) |
| title_full | Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (<it>Puccinia striiformis </it>f. sp. <it>tritici</it>) |
| title_fullStr | Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (<it>Puccinia striiformis </it>f. sp. <it>tritici</it>) |
| title_full_unstemmed | Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (<it>Puccinia striiformis </it>f. sp. <it>tritici</it>) |
| title_short | Construction and characterization of a full-length cDNA library for the wheat stripe rust pathogen (<it>Puccinia striiformis </it>f. sp. <it>tritici</it>) |
| title_sort | construction and characterization of a full length cdna library for the wheat stripe rust pathogen it puccinia striiformis it f sp it tritici it |
| url | http://www.biomedcentral.com/1471-2164/8/145 |
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