Altered neurological function in mice immunized with early endosome antigen 1
<p>Abstract</p> <p>Background</p> <p>Autoantibodies directed against the 160 kDa endosome protein early endosome antigen 1 (EEA1) are seen in patients with neurological diseases. To determine if antibodies to EEA1 have a neuropathological effect, mice from three major h...
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| Format: | Article |
| Language: | English |
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BMC
2004-01-01
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| Series: | BMC Neuroscience |
| Subjects: | |
| Online Access: | http://www.biomedcentral.com/1471-2202/5/2 |
| _version_ | 1811299323605942272 |
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| author | Fritzler Marvin J Selak Sanja |
| author_facet | Fritzler Marvin J Selak Sanja |
| author_sort | Fritzler Marvin J |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Autoantibodies directed against the 160 kDa endosome protein early endosome antigen 1 (EEA1) are seen in patients with neurological diseases. To determine if antibodies to EEA1 have a neuropathological effect, mice from three major histocompatability haplotype backgrounds (H2<sup>q</sup>, H2<sup>b </sup>and H2<sup>d</sup>) were immunized with EEA1 (amino acids 82–1411) that was previously shown to contain the target EEA1 epitopes. The mice were then subjected to five neuro-behavioural tests: grid walking, forelimb strength, open field, reaching and rotarod.</p> <p>Results</p> <p>The immunized SWR/J mice with sustained anti-EEA1 antibodies had significantly reduced forelimb strength than the control non-immune mice of the same strain, and BALB/CJ immune mice demonstrated significantly more forelimb errors on the grid walk test than the control group.</p> <p>Conclusions</p> <p>Antibodies to recombinant EEA1 in mice may mediate neurological deficits that are consistent with clinical features of some humans that spontaneously develop anti-EEA1 autoantibodies.</p> |
| first_indexed | 2024-04-13T06:34:27Z |
| format | Article |
| id | doaj.art-205ebcdb71c3489e8201a81e76d85256 |
| institution | Directory Open Access Journal |
| issn | 1471-2202 |
| language | English |
| last_indexed | 2024-04-13T06:34:27Z |
| publishDate | 2004-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Neuroscience |
| spelling | doaj.art-205ebcdb71c3489e8201a81e76d852562022-12-22T02:57:59ZengBMCBMC Neuroscience1471-22022004-01-0151210.1186/1471-2202-5-2Altered neurological function in mice immunized with early endosome antigen 1Fritzler Marvin JSelak Sanja<p>Abstract</p> <p>Background</p> <p>Autoantibodies directed against the 160 kDa endosome protein early endosome antigen 1 (EEA1) are seen in patients with neurological diseases. To determine if antibodies to EEA1 have a neuropathological effect, mice from three major histocompatability haplotype backgrounds (H2<sup>q</sup>, H2<sup>b </sup>and H2<sup>d</sup>) were immunized with EEA1 (amino acids 82–1411) that was previously shown to contain the target EEA1 epitopes. The mice were then subjected to five neuro-behavioural tests: grid walking, forelimb strength, open field, reaching and rotarod.</p> <p>Results</p> <p>The immunized SWR/J mice with sustained anti-EEA1 antibodies had significantly reduced forelimb strength than the control non-immune mice of the same strain, and BALB/CJ immune mice demonstrated significantly more forelimb errors on the grid walk test than the control group.</p> <p>Conclusions</p> <p>Antibodies to recombinant EEA1 in mice may mediate neurological deficits that are consistent with clinical features of some humans that spontaneously develop anti-EEA1 autoantibodies.</p>http://www.biomedcentral.com/1471-2202/5/2AutoimmunityAutoantibodiesEndosomeNeurological FunctionAnimal Model |
| spellingShingle | Fritzler Marvin J Selak Sanja Altered neurological function in mice immunized with early endosome antigen 1 BMC Neuroscience Autoimmunity Autoantibodies Endosome Neurological Function Animal Model |
| title | Altered neurological function in mice immunized with early endosome antigen 1 |
| title_full | Altered neurological function in mice immunized with early endosome antigen 1 |
| title_fullStr | Altered neurological function in mice immunized with early endosome antigen 1 |
| title_full_unstemmed | Altered neurological function in mice immunized with early endosome antigen 1 |
| title_short | Altered neurological function in mice immunized with early endosome antigen 1 |
| title_sort | altered neurological function in mice immunized with early endosome antigen 1 |
| topic | Autoimmunity Autoantibodies Endosome Neurological Function Animal Model |
| url | http://www.biomedcentral.com/1471-2202/5/2 |
| work_keys_str_mv | AT fritzlermarvinj alteredneurologicalfunctioninmiceimmunizedwithearlyendosomeantigen1 AT selaksanja alteredneurologicalfunctioninmiceimmunizedwithearlyendosomeantigen1 |