Immunomodulatory activity of glycoproteins isolated from chickpea (Cicer arietinum L.)
Chickpea (Cicer arietinum L.) is a well-known legume widely used as traditional medicine. This study aimed to characterize the structure and evaluate the immunomodulatory activity of one glycoprotein [crude chickpea glycoprotein-1 (CAG-1)] isolated from chickpea. CAG-1 was extracted with hot alkalin...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-09-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.966705/full |
| _version_ | 1798033754390790144 |
|---|---|
| author | Zhenxing Shi Zhenxing Shi Zhenxing Shi Shiyu Li Zuchen Wei Zuchen Wei Yuanji Wang Nong Zhou Qiang Ma Yang Yao |
| author_facet | Zhenxing Shi Zhenxing Shi Zhenxing Shi Shiyu Li Zuchen Wei Zuchen Wei Yuanji Wang Nong Zhou Qiang Ma Yang Yao |
| author_sort | Zhenxing Shi |
| collection | DOAJ |
| description | Chickpea (Cicer arietinum L.) is a well-known legume widely used as traditional medicine. This study aimed to characterize the structure and evaluate the immunomodulatory activity of one glycoprotein [crude chickpea glycoprotein-1 (CAG-1)] isolated from chickpea. CAG-1 was extracted with hot alkaline water and purified with DEAE-Sepharose Fast Flow and Superdex-200 column chromatography. CAG-1, with a molecular weight of 8,106 Da, contained 57.12% polysaccharide and 35.41% protein. The polysaccharide part was mainly composed of glucose (Glc). The protein part was connected mainly by aspartic (Asp) and glutamic (Glu). The results of nuclear magnetic resonance (NMR) analysis indicated the presence of α-d-Glcp-(1 → 4)-α-d-Glcp-(1 → 4)-α-d-Glcp-(1 → . In addition, the sugar chains of the glycoprotein were not hydrolyzed under alkaline conditions, suggesting that the glycoprotein was N-glycosidic; thus, the sugar chain was linked to the protein chain by Asp. An immunological study showed that CAG-1 stimulated the production of nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and monocyte chemotactic protein 1 (MCP-1) in RAW 264.7 macrophages in a dose-dependent manner. |
| first_indexed | 2024-04-11T20:34:54Z |
| format | Article |
| id | doaj.art-205f194ddb4c4e9c8a850a3e135f74c0 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-11T20:34:54Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-205f194ddb4c4e9c8a850a3e135f74c02022-12-22T04:04:24ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-09-01910.3389/fnut.2022.966705966705Immunomodulatory activity of glycoproteins isolated from chickpea (Cicer arietinum L.)Zhenxing Shi0Zhenxing Shi1Zhenxing Shi2Shiyu Li3Zuchen Wei4Zuchen Wei5Yuanji Wang6Nong Zhou7Qiang Ma8Yang Yao9Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaDepartment of Basic Medicine, Chongqing Three Gorges Medical College, Chongqing, ChinaSchool of Food Science and Technology, Henan University of Technology, Zhengzhou, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory for Green Cultivation and Deep Processing of Three Gorges Reservoir Area's Medicinal Herbs, College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaLaboratory for Green Cultivation and Deep Processing of Three Gorges Reservoir Area's Medicinal Herbs, College of Life Science and Engineering, Chongqing Three Gorges University, Chongqing, ChinaDepartment of Basic Medicine, Chongqing Three Gorges Medical College, Chongqing, ChinaInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaChickpea (Cicer arietinum L.) is a well-known legume widely used as traditional medicine. This study aimed to characterize the structure and evaluate the immunomodulatory activity of one glycoprotein [crude chickpea glycoprotein-1 (CAG-1)] isolated from chickpea. CAG-1 was extracted with hot alkaline water and purified with DEAE-Sepharose Fast Flow and Superdex-200 column chromatography. CAG-1, with a molecular weight of 8,106 Da, contained 57.12% polysaccharide and 35.41% protein. The polysaccharide part was mainly composed of glucose (Glc). The protein part was connected mainly by aspartic (Asp) and glutamic (Glu). The results of nuclear magnetic resonance (NMR) analysis indicated the presence of α-d-Glcp-(1 → 4)-α-d-Glcp-(1 → 4)-α-d-Glcp-(1 → . In addition, the sugar chains of the glycoprotein were not hydrolyzed under alkaline conditions, suggesting that the glycoprotein was N-glycosidic; thus, the sugar chain was linked to the protein chain by Asp. An immunological study showed that CAG-1 stimulated the production of nitric oxide (NO), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and monocyte chemotactic protein 1 (MCP-1) in RAW 264.7 macrophages in a dose-dependent manner.https://www.frontiersin.org/articles/10.3389/fnut.2022.966705/fullglycoproteinstructural characterizationimmunomodulatoryRAW 264.7 murine macrophage cellchickpea |
| spellingShingle | Zhenxing Shi Zhenxing Shi Zhenxing Shi Shiyu Li Zuchen Wei Zuchen Wei Yuanji Wang Nong Zhou Qiang Ma Yang Yao Immunomodulatory activity of glycoproteins isolated from chickpea (Cicer arietinum L.) Frontiers in Nutrition glycoprotein structural characterization immunomodulatory RAW 264.7 murine macrophage cell chickpea |
| title | Immunomodulatory activity of glycoproteins isolated from chickpea (Cicer arietinum L.) |
| title_full | Immunomodulatory activity of glycoproteins isolated from chickpea (Cicer arietinum L.) |
| title_fullStr | Immunomodulatory activity of glycoproteins isolated from chickpea (Cicer arietinum L.) |
| title_full_unstemmed | Immunomodulatory activity of glycoproteins isolated from chickpea (Cicer arietinum L.) |
| title_short | Immunomodulatory activity of glycoproteins isolated from chickpea (Cicer arietinum L.) |
| title_sort | immunomodulatory activity of glycoproteins isolated from chickpea cicer arietinum l |
| topic | glycoprotein structural characterization immunomodulatory RAW 264.7 murine macrophage cell chickpea |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.966705/full |
| work_keys_str_mv | AT zhenxingshi immunomodulatoryactivityofglycoproteinsisolatedfromchickpeacicerarietinuml AT zhenxingshi immunomodulatoryactivityofglycoproteinsisolatedfromchickpeacicerarietinuml AT zhenxingshi immunomodulatoryactivityofglycoproteinsisolatedfromchickpeacicerarietinuml AT shiyuli immunomodulatoryactivityofglycoproteinsisolatedfromchickpeacicerarietinuml AT zuchenwei immunomodulatoryactivityofglycoproteinsisolatedfromchickpeacicerarietinuml AT zuchenwei immunomodulatoryactivityofglycoproteinsisolatedfromchickpeacicerarietinuml AT yuanjiwang immunomodulatoryactivityofglycoproteinsisolatedfromchickpeacicerarietinuml AT nongzhou immunomodulatoryactivityofglycoproteinsisolatedfromchickpeacicerarietinuml AT qiangma immunomodulatoryactivityofglycoproteinsisolatedfromchickpeacicerarietinuml AT yangyao immunomodulatoryactivityofglycoproteinsisolatedfromchickpeacicerarietinuml |