Systemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model

Systemic Optimization of Gene Electrotransfer Protocol Using Hard-to-Transfect UT-7 Cell Line as a Model

Non-adherent cells are difficult to transfect with chemical-mediated delivery methods. Electroporation is an attractive strategy to transfer the molecules of interest into suspension cells. Care must be taken with the viability of the transfected cells since parameters, which increase cell membrane...

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Bibliographic Details
Main Authors: Roberta Vadeikienė, Baltramiejus Jakštys, Rasa Ugenskienė, Saulius Šatkauskas, Elona Juozaitytė
Format: Article
Language:English
Published: MDPI AG 2022-10-01
Series:Biomedicines
Subjects:
electroporation
optimization
plasmid DNA transfer
gene electrotransfer (GET)
non-adherent cells
UT-7 cell line
Online Access:https://www.mdpi.com/2227-9059/10/11/2687

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