Producción de polihidroxialcanoatos por bacterias halófilas nativas utilizando almidón de cáscaras de Solanum tuberosum L.
The aim of this study was to determine the concentration of starch Solanum tuberosum L. “potato” peels for the production of polyhydroxyalkanoates, PHA, from native halophilic bacteria as an alternative to reduce production costs of these biopolymers, possible replacem...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Universidad Nacional de Trujillo
2017-01-01
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| Series: | Scientia Agropecuaria |
| Online Access: | http://www.redalyc.org/articulo.oa?id=357651874003 |
| _version_ | 1818042549721366528 |
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| author | César Guzmán Alberto Hurtado Carmen Carreño Indira Casos |
| author_facet | César Guzmán Alberto Hurtado Carmen Carreño Indira Casos |
| author_sort | César Guzmán |
| collection | DOAJ |
| description | The aim of this study was to determine the concentration of starch Solanum tuberosum L. “potato” peels for the production of polyhydroxyalkanoates, PHA, from native halophilic bacteria as an alternative to reduce production costs of these biopolymers, possible replacements for petrochemical plastics. The bacteria were isolated of water samples of eight saline Lamba yeque region and were enriched in HM 1 broth at 30 °C with 5, 10, 15, 20, 25 and 30 g 100 mL - 1 NaCl. Dilutions were performed subsequently, aliquots were taken and plated on HM 1 agar, 203 isolates of halophilic bacteria were obtained, they were grown in HM 2 broth with 10 g.L - 1 glucose as carbon source and the 38.92% of the isolates showed PHA granules stained by Sudan Black B. Twenty bacteria with PHA granules in 65 – 75% over carried to fermentation, reaching between 0.174 to 0.889 g . g - 1 of yield Y (p / x). Three isolates were selected with the highest values among which Halomonas sp M4C1 grew and synthesized PHA in HM 2 broth with 5, 10, 15 and 20 g . L - 1 of starch as carbon source, reaching 0.019; 0.016; 0.007 y 0.006 g . L - 1 of PHA, with 0.177; 0.111; 0.056 an d 0.066 g . L - 1 of biomass after 20, 40, 24 and 16 hours respectively. The highest yield of 0.144 g . g - 1 corresponded to 10 g . L - 1 of starch demonstrating that this concentration is feasible PHA production by native halophilic bacteria. |
| first_indexed | 2024-12-10T08:48:05Z |
| format | Article |
| id | doaj.art-206e08c167e8455c9585443ba0ee3c57 |
| institution | Directory Open Access Journal |
| issn | 2077-9917 2306-6741 |
| language | English |
| last_indexed | 2024-12-10T08:48:05Z |
| publishDate | 2017-01-01 |
| publisher | Universidad Nacional de Trujillo |
| record_format | Article |
| series | Scientia Agropecuaria |
| spelling | doaj.art-206e08c167e8455c9585443ba0ee3c572022-12-22T01:55:41ZengUniversidad Nacional de TrujilloScientia Agropecuaria2077-99172306-67412017-01-0182109118Producción de polihidroxialcanoatos por bacterias halófilas nativas utilizando almidón de cáscaras de Solanum tuberosum L.César GuzmánAlberto HurtadoCarmen CarreñoIndira CasosThe aim of this study was to determine the concentration of starch Solanum tuberosum L. “potato” peels for the production of polyhydroxyalkanoates, PHA, from native halophilic bacteria as an alternative to reduce production costs of these biopolymers, possible replacements for petrochemical plastics. The bacteria were isolated of water samples of eight saline Lamba yeque region and were enriched in HM 1 broth at 30 °C with 5, 10, 15, 20, 25 and 30 g 100 mL - 1 NaCl. Dilutions were performed subsequently, aliquots were taken and plated on HM 1 agar, 203 isolates of halophilic bacteria were obtained, they were grown in HM 2 broth with 10 g.L - 1 glucose as carbon source and the 38.92% of the isolates showed PHA granules stained by Sudan Black B. Twenty bacteria with PHA granules in 65 – 75% over carried to fermentation, reaching between 0.174 to 0.889 g . g - 1 of yield Y (p / x). Three isolates were selected with the highest values among which Halomonas sp M4C1 grew and synthesized PHA in HM 2 broth with 5, 10, 15 and 20 g . L - 1 of starch as carbon source, reaching 0.019; 0.016; 0.007 y 0.006 g . L - 1 of PHA, with 0.177; 0.111; 0.056 an d 0.066 g . L - 1 of biomass after 20, 40, 24 and 16 hours respectively. The highest yield of 0.144 g . g - 1 corresponded to 10 g . L - 1 of starch demonstrating that this concentration is feasible PHA production by native halophilic bacteria.http://www.redalyc.org/articulo.oa?id=357651874003 |
| spellingShingle | César Guzmán Alberto Hurtado Carmen Carreño Indira Casos Producción de polihidroxialcanoatos por bacterias halófilas nativas utilizando almidón de cáscaras de Solanum tuberosum L. Scientia Agropecuaria |
| title | Producción de polihidroxialcanoatos por bacterias halófilas nativas utilizando almidón de cáscaras de Solanum tuberosum L. |
| title_full | Producción de polihidroxialcanoatos por bacterias halófilas nativas utilizando almidón de cáscaras de Solanum tuberosum L. |
| title_fullStr | Producción de polihidroxialcanoatos por bacterias halófilas nativas utilizando almidón de cáscaras de Solanum tuberosum L. |
| title_full_unstemmed | Producción de polihidroxialcanoatos por bacterias halófilas nativas utilizando almidón de cáscaras de Solanum tuberosum L. |
| title_short | Producción de polihidroxialcanoatos por bacterias halófilas nativas utilizando almidón de cáscaras de Solanum tuberosum L. |
| title_sort | produccion de polihidroxialcanoatos por bacterias halofilas nativas utilizando almidon de cascaras de solanum tuberosum l |
| url | http://www.redalyc.org/articulo.oa?id=357651874003 |
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