INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS
Modulatory effects of three probiotic bacterial strains (Lactobacillus rhamnosus K32 (L), Bifidobacterium longum GT15 (B, Enterococcus faecium L-3 (E) on expression level and contents of key cytokines were studied using PCR techniques with reverse transcription, and enzyme-linked immunosorbent assay...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2015-10-01
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| Series: | Медицинская иммунология |
| Subjects: | |
| Online Access: | https://www.mimmun.ru/mimmun/article/view/932 |
| _version_ | 1826534245293621248 |
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| author | O. V. Averina E. I. Ermolenko A. Yu. Ratushniy E. A. Tarasova Yu. Yu. Borschev G. F. Leontieva T. A. Kramskaya M. P. Kotyleva V. N. Danilenko A. N. Suvorov |
| author_facet | O. V. Averina E. I. Ermolenko A. Yu. Ratushniy E. A. Tarasova Yu. Yu. Borschev G. F. Leontieva T. A. Kramskaya M. P. Kotyleva V. N. Danilenko A. N. Suvorov |
| author_sort | O. V. Averina |
| collection | DOAJ |
| description | Modulatory effects of three probiotic bacterial strains (Lactobacillus rhamnosus K32 (L), Bifidobacterium longum GT15 (B, Enterococcus faecium L-3 (E) on expression level and contents of key cytokines were studied using PCR techniques with reverse transcription, and enzyme-linked immunosorbent assay. Both cell cultures and an experimental model of intestinal dysbiosis were used in this study.The genes encoding bacteriocins, surface membrane component, pili and exopolysaccharides involved in host immune system modulation were previously identified in the B and Ebacterial strains.Investigation of probiotic strains and effects of their supernatants expression of cytokines in cell cultures of promonocyte origin (HTP-1) showed increased expression of TNFα, due to E and L supernatants. Moreover, the Bl culture induced IL-8 and IL-10 expression.In a model of Wistar rats with ampicillinand metronidazole-induced intestinal dysbiosis corrected with probiotics we have shown that the dysbiosis was accompanied by sufficient alterations in microbiota composition (Klebsiella spp. overgrowth and low contents of Faecalobacterium prausnitzii) that were observed only in the animals untreated with probiotics (control), or after administration of L.In contrast to these results, the animals treated with E and B, the following changes were revealed: 1) low expression of proinflammatory cytokines IL-8, TNFα, MCP-1 inmesenteric lymph nodes and appropriate changes of their serum contents, 2) increased serum content of the anti-inflammatory TGFβ cytokine. Hence, the present study, having used two complementary models, has detected some individual features of immune modulation produced by the probiotictic strains of L. rhamnosus K32, B. longum GT15 и E. faecium L-3 which exert differential effects upon the intestinal microbiota. |
| first_indexed | 2024-03-08T05:49:25Z |
| format | Article |
| id | doaj.art-206ee7df86b245d8a113f1d53ceba008 |
| institution | Directory Open Access Journal |
| issn | 1563-0625 2313-741X |
| language | Russian |
| last_indexed | 2025-03-14T02:19:57Z |
| publishDate | 2015-10-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj.art-206ee7df86b245d8a113f1d53ceba0082025-03-11T17:59:06ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2015-10-0117544345410.15789/1563-0625-2015-5-443-454801INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMSO. V. Averina0E. I. Ermolenko1A. Yu. Ratushniy2E. A. Tarasova3Yu. Yu. Borschev4G. F. Leontieva5T. A. Kramskaya6M. P. Kotyleva7V. N. Danilenko8A. N. Suvorov9N.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, MoscowInstitute of Experimental Medicine, St. Petersburg; Saint Petersburg State UniversityN.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, MoscowInstitute of Experimental Medicine, St. PetersburgInstitute of Experimental Medicine, St. PetersburgInstitute of Experimental Medicine, St. PetersburgInstitute of Experimental Medicine, St. PetersburgInstitute of Experimental Medicine, St. Petersburg; Saint Petersburg State UniversityN.I. Vavilov Institute of General Genetics, Russian Academy of Sciences, MoscowInstitute of Experimental Medicine, St. Petersburg; Saint Petersburg State UniversityModulatory effects of three probiotic bacterial strains (Lactobacillus rhamnosus K32 (L), Bifidobacterium longum GT15 (B, Enterococcus faecium L-3 (E) on expression level and contents of key cytokines were studied using PCR techniques with reverse transcription, and enzyme-linked immunosorbent assay. Both cell cultures and an experimental model of intestinal dysbiosis were used in this study.The genes encoding bacteriocins, surface membrane component, pili and exopolysaccharides involved in host immune system modulation were previously identified in the B and Ebacterial strains.Investigation of probiotic strains and effects of their supernatants expression of cytokines in cell cultures of promonocyte origin (HTP-1) showed increased expression of TNFα, due to E and L supernatants. Moreover, the Bl culture induced IL-8 and IL-10 expression.In a model of Wistar rats with ampicillinand metronidazole-induced intestinal dysbiosis corrected with probiotics we have shown that the dysbiosis was accompanied by sufficient alterations in microbiota composition (Klebsiella spp. overgrowth and low contents of Faecalobacterium prausnitzii) that were observed only in the animals untreated with probiotics (control), or after administration of L.In contrast to these results, the animals treated with E and B, the following changes were revealed: 1) low expression of proinflammatory cytokines IL-8, TNFα, MCP-1 inmesenteric lymph nodes and appropriate changes of their serum contents, 2) increased serum content of the anti-inflammatory TGFβ cytokine. Hence, the present study, having used two complementary models, has detected some individual features of immune modulation produced by the probiotictic strains of L. rhamnosus K32, B. longum GT15 и E. faecium L-3 which exert differential effects upon the intestinal microbiota.https://www.mimmun.ru/mimmun/article/view/932lactobacillibifidobacteriaenterococcidysbiosiscytokines |
| spellingShingle | O. V. Averina E. I. Ermolenko A. Yu. Ratushniy E. A. Tarasova Yu. Yu. Borschev G. F. Leontieva T. A. Kramskaya M. P. Kotyleva V. N. Danilenko A. N. Suvorov INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS Медицинская иммунология lactobacilli bifidobacteria enterococci dysbiosis cytokines |
| title | INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS |
| title_full | INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS |
| title_fullStr | INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS |
| title_full_unstemmed | INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS |
| title_short | INFLUENCE OF PROBIOTICS ON CYTOKINE PRODUCTION IN THE IN VITRO AND IN VIVO SYSTEMS |
| title_sort | influence of probiotics on cytokine production in the in vitro and in vivo systems |
| topic | lactobacilli bifidobacteria enterococci dysbiosis cytokines |
| url | https://www.mimmun.ru/mimmun/article/view/932 |
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