Screening of Ultraviolet-Induced Thermotolerant Yeast Mutants and Their Performance
The simultaneous saccharification and fermentation (SSF) technique holds promise for the conversion of lignocellulose to ethanol. However, the optimal fermentation temperature of yeast is lower than the enzymatic hydrolysis temperature of the saccharification process, which leads to the temperature...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-06-01
|
| Series: | Fermentation |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2311-5637/9/7/608 |
| _version_ | 1827733036567887872 |
|---|---|
| author | Xiaodi Li Yan Lin Hainan Kong Zhiquan Wang |
| author_facet | Xiaodi Li Yan Lin Hainan Kong Zhiquan Wang |
| author_sort | Xiaodi Li |
| collection | DOAJ |
| description | The simultaneous saccharification and fermentation (SSF) technique holds promise for the conversion of lignocellulose to ethanol. However, the optimal fermentation temperature of yeast is lower than the enzymatic hydrolysis temperature of the saccharification process, which leads to the temperature of the actual production process of SSF usually being lower than 38 °C. In this work, two ultraviolet (UV)-induced mutations were performed step by step using <i>Saccharomyces cerevisiae</i> BY4742 as the original strain to enable the yeast to perform well at higher temperatures. Thermotolerant strains obtained through mutagenesis and screening, YUV1-1 and YUV2-2, were utilized for fermentation and SSF at a targeted temperature of 40 °C. They obtained ethanol yields comparable to those at 38 °C in SSF, whereas the ethanol yields of the original strain at 40 °C decreased by about 10% compared to those at 38 °C. This study proves that thermotolerant strains adapted to elevated fermentation and SSF temperatures can be obtained through UV mutagenesis and screening, thereby increasing the stability of the fermentation and SSF processes and lowering the subsequent distillation costs. |
| first_indexed | 2024-03-11T01:05:25Z |
| format | Article |
| id | doaj.art-206f75d150cf435f9a30927d2efb78a6 |
| institution | Directory Open Access Journal |
| issn | 2311-5637 |
| language | English |
| last_indexed | 2024-03-11T01:05:25Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Fermentation |
| spelling | doaj.art-206f75d150cf435f9a30927d2efb78a62023-11-18T19:15:52ZengMDPI AGFermentation2311-56372023-06-019760810.3390/fermentation9070608Screening of Ultraviolet-Induced Thermotolerant Yeast Mutants and Their PerformanceXiaodi Li0Yan Lin1Hainan Kong2Zhiquan Wang3State Key Laboratory of Pollution Control and Resource Reuse, College of Environmental Science and Engineering, Tongji University, Shanghai 200092, ChinaSchool of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, ChinaSchool of Environmental Science and Engineering, Shanghai Jiao Tong University, Shanghai 200240, ChinaCollege of Life and Environmental Science, Wenzhou University, Wenzhou 325035, ChinaThe simultaneous saccharification and fermentation (SSF) technique holds promise for the conversion of lignocellulose to ethanol. However, the optimal fermentation temperature of yeast is lower than the enzymatic hydrolysis temperature of the saccharification process, which leads to the temperature of the actual production process of SSF usually being lower than 38 °C. In this work, two ultraviolet (UV)-induced mutations were performed step by step using <i>Saccharomyces cerevisiae</i> BY4742 as the original strain to enable the yeast to perform well at higher temperatures. Thermotolerant strains obtained through mutagenesis and screening, YUV1-1 and YUV2-2, were utilized for fermentation and SSF at a targeted temperature of 40 °C. They obtained ethanol yields comparable to those at 38 °C in SSF, whereas the ethanol yields of the original strain at 40 °C decreased by about 10% compared to those at 38 °C. This study proves that thermotolerant strains adapted to elevated fermentation and SSF temperatures can be obtained through UV mutagenesis and screening, thereby increasing the stability of the fermentation and SSF processes and lowering the subsequent distillation costs.https://www.mdpi.com/2311-5637/9/7/608ultraviolet mutagenesissimultaneous saccharification and fermentationbioethanolthermotolerant yeast |
| spellingShingle | Xiaodi Li Yan Lin Hainan Kong Zhiquan Wang Screening of Ultraviolet-Induced Thermotolerant Yeast Mutants and Their Performance Fermentation ultraviolet mutagenesis simultaneous saccharification and fermentation bioethanol thermotolerant yeast |
| title | Screening of Ultraviolet-Induced Thermotolerant Yeast Mutants and Their Performance |
| title_full | Screening of Ultraviolet-Induced Thermotolerant Yeast Mutants and Their Performance |
| title_fullStr | Screening of Ultraviolet-Induced Thermotolerant Yeast Mutants and Their Performance |
| title_full_unstemmed | Screening of Ultraviolet-Induced Thermotolerant Yeast Mutants and Their Performance |
| title_short | Screening of Ultraviolet-Induced Thermotolerant Yeast Mutants and Their Performance |
| title_sort | screening of ultraviolet induced thermotolerant yeast mutants and their performance |
| topic | ultraviolet mutagenesis simultaneous saccharification and fermentation bioethanol thermotolerant yeast |
| url | https://www.mdpi.com/2311-5637/9/7/608 |
| work_keys_str_mv | AT xiaodili screeningofultravioletinducedthermotolerantyeastmutantsandtheirperformance AT yanlin screeningofultravioletinducedthermotolerantyeastmutantsandtheirperformance AT hainankong screeningofultravioletinducedthermotolerantyeastmutantsandtheirperformance AT zhiquanwang screeningofultravioletinducedthermotolerantyeastmutantsandtheirperformance |