Quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly Megaselia abdita
The segmentation gene network in insects can produce equivalent phenotypic outputs despite differences in upstream regulatory inputs between species. We investigate the mechanistic basis of this phenomenon through a systems-level analysis of the gap gene network in the scuttle fly Megaselia abdita (...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2015-01-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/04785 |
| _version_ | 1811200337611063296 |
|---|---|
| author | Karl R Wotton Eva Jiménez-Guri Anton Crombach Hilde Janssens Anna Alcaine-Colet Steffen Lemke Urs Schmidt-Ott Johannes Jaeger |
| author_facet | Karl R Wotton Eva Jiménez-Guri Anton Crombach Hilde Janssens Anna Alcaine-Colet Steffen Lemke Urs Schmidt-Ott Johannes Jaeger |
| author_sort | Karl R Wotton |
| collection | DOAJ |
| description | The segmentation gene network in insects can produce equivalent phenotypic outputs despite differences in upstream regulatory inputs between species. We investigate the mechanistic basis of this phenomenon through a systems-level analysis of the gap gene network in the scuttle fly Megaselia abdita (Phoridae). It combines quantification of gene expression at high spatio-temporal resolution with systematic knock-downs by RNA interference (RNAi). Initiation and dynamics of gap gene expression differ markedly between M. abdita and Drosophila melanogaster, while the output of the system converges to equivalent patterns at the end of the blastoderm stage. Although the qualitative structure of the gap gene network is conserved, there are differences in the strength of regulatory interactions between species. We term such network rewiring ‘quantitative system drift’. It provides a mechanistic explanation for the developmental hourglass model in the dipteran lineage. Quantitative system drift is likely to be a widespread mechanism for developmental evolution. |
| first_indexed | 2024-04-12T02:03:10Z |
| format | Article |
| id | doaj.art-2070acfada30441ab4cc352781a9eac7 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:03:10Z |
| publishDate | 2015-01-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-2070acfada30441ab4cc352781a9eac72022-12-22T03:52:37ZengeLife Sciences Publications LtdeLife2050-084X2015-01-01410.7554/eLife.04785Quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly Megaselia abditaKarl R Wotton0https://orcid.org/0000-0002-8672-9948Eva Jiménez-Guri1https://orcid.org/0000-0002-9592-1077Anton Crombach2Hilde Janssens3Anna Alcaine-Colet4Steffen Lemke5Urs Schmidt-Ott6Johannes Jaeger7https://orcid.org/0000-0002-2568-2103European Molecular Biology Laboratory, CRG Systems Biology Research Unit, Centre for Genomic Regulation, Barcelona, Spain; Universitat Pompeu Fabra, Barcelona, SpainEuropean Molecular Biology Laboratory, CRG Systems Biology Research Unit, Centre for Genomic Regulation, Barcelona, Spain; Universitat Pompeu Fabra, Barcelona, SpainEuropean Molecular Biology Laboratory, CRG Systems Biology Research Unit, Centre for Genomic Regulation, Barcelona, Spain; Universitat Pompeu Fabra, Barcelona, SpainEuropean Molecular Biology Laboratory, CRG Systems Biology Research Unit, Centre for Genomic Regulation, Barcelona, Spain; Universitat Pompeu Fabra, Barcelona, SpainEuropean Molecular Biology Laboratory, CRG Systems Biology Research Unit, Centre for Genomic Regulation, Barcelona, Spain; Universitat Pompeu Fabra, Barcelona, Spain; Universitat de Barcelona, Barcelona, SpainDepartment of Organismal Biology and Anatomy, University of Chicago, Chicago, United StatesDepartment of Organismal Biology and Anatomy, University of Chicago, Chicago, United StatesEuropean Molecular Biology Laboratory, CRG Systems Biology Research Unit, Centre for Genomic Regulation, Barcelona, Spain; Universitat Pompeu Fabra, Barcelona, SpainThe segmentation gene network in insects can produce equivalent phenotypic outputs despite differences in upstream regulatory inputs between species. We investigate the mechanistic basis of this phenomenon through a systems-level analysis of the gap gene network in the scuttle fly Megaselia abdita (Phoridae). It combines quantification of gene expression at high spatio-temporal resolution with systematic knock-downs by RNA interference (RNAi). Initiation and dynamics of gap gene expression differ markedly between M. abdita and Drosophila melanogaster, while the output of the system converges to equivalent patterns at the end of the blastoderm stage. Although the qualitative structure of the gap gene network is conserved, there are differences in the strength of regulatory interactions between species. We term such network rewiring ‘quantitative system drift’. It provides a mechanistic explanation for the developmental hourglass model in the dipteran lineage. Quantitative system drift is likely to be a widespread mechanism for developmental evolution.https://elifesciences.org/articles/04785evolutionary developmental biologydevelopmental system driftgap gene networkRNA interferencedata quantificationMegaselia abdita |
| spellingShingle | Karl R Wotton Eva Jiménez-Guri Anton Crombach Hilde Janssens Anna Alcaine-Colet Steffen Lemke Urs Schmidt-Ott Johannes Jaeger Quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly Megaselia abdita eLife evolutionary developmental biology developmental system drift gap gene network RNA interference data quantification Megaselia abdita |
| title | Quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly Megaselia abdita |
| title_full | Quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly Megaselia abdita |
| title_fullStr | Quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly Megaselia abdita |
| title_full_unstemmed | Quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly Megaselia abdita |
| title_short | Quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly Megaselia abdita |
| title_sort | quantitative system drift compensates for altered maternal inputs to the gap gene network of the scuttle fly megaselia abdita |
| topic | evolutionary developmental biology developmental system drift gap gene network RNA interference data quantification Megaselia abdita |
| url | https://elifesciences.org/articles/04785 |
| work_keys_str_mv | AT karlrwotton quantitativesystemdriftcompensatesforalteredmaternalinputstothegapgenenetworkofthescuttleflymegaseliaabdita AT evajimenezguri quantitativesystemdriftcompensatesforalteredmaternalinputstothegapgenenetworkofthescuttleflymegaseliaabdita AT antoncrombach quantitativesystemdriftcompensatesforalteredmaternalinputstothegapgenenetworkofthescuttleflymegaseliaabdita AT hildejanssens quantitativesystemdriftcompensatesforalteredmaternalinputstothegapgenenetworkofthescuttleflymegaseliaabdita AT annaalcainecolet quantitativesystemdriftcompensatesforalteredmaternalinputstothegapgenenetworkofthescuttleflymegaseliaabdita AT steffenlemke quantitativesystemdriftcompensatesforalteredmaternalinputstothegapgenenetworkofthescuttleflymegaseliaabdita AT ursschmidtott quantitativesystemdriftcompensatesforalteredmaternalinputstothegapgenenetworkofthescuttleflymegaseliaabdita AT johannesjaeger quantitativesystemdriftcompensatesforalteredmaternalinputstothegapgenenetworkofthescuttleflymegaseliaabdita |