Transplantation of adiponectin-transduced endothelial progenitor cells improves neurobehavioral outcomes following transient middle cerebral artery occlusion
Objective: To observe the effect of APN-modified EPCs on brain tissue repair and neurological function recovery in ischemic stroke model mice. Methods: Construction of lentiviruses transfected with APN genes. Genetically modified EPCs overexpressing APN(EPC-APN) or green fluorescent protein (EPC-GFP...
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of Diagnostics Concepts & Practice
2022-12-01
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| Series: | Zhenduanxue lilun yu shijian |
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| Online Access: | https://www.qk.sjtu.edu.cn/jdcp/fileup/1671-2870/PDF/1682220171051-753291557.pdf |
| _version_ | 1797840622428618752 |
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| author | SHEN Linhui, WANG Shuhong, MIAO Jie |
| author_facet | SHEN Linhui, WANG Shuhong, MIAO Jie |
| author_sort | SHEN Linhui, WANG Shuhong, MIAO Jie |
| collection | DOAJ |
| description | Objective: To observe the effect of APN-modified EPCs on brain tissue repair and neurological function recovery in ischemic stroke model mice. Methods: Construction of lentiviruses transfected with APN genes. Genetically modified EPCs overexpressing APN(EPC-APN) or green fluorescent protein (EPC-GFP) were transplanted into a mice model of transient middle cerebral artery occlusion (tMCAO). The EPC-APN transplantation group, EPC-green fluorescent protein (GFP) transplantation control group and phosphate buffer (PBS) control treatment group were established. After transplantation, the enhanced GFP-expressing cells were found at the peri-infact area of brain. At 1, 7 and 14 days after tMCAO, the infarct volume was measured by using cresyl violet. Angiogenesis in the perifocal region was quantified on the 14th day. The apoptosis of endogenous cells was evaluated using TUNEL assay. Expression of vascular endothelial growth factor（VEGF）was detected using Western blot. Results: By constructing APN gene lentiviral vectors to transfect to EPCs, EPCs overexpressing APN were successfully prepared. The volume of brain atrophy in the EPC-APN transplantation group on on the 14th day after cerebral ischemia was significantly reduced compared with that in the EPC-GFP transplantation control group and the PBS control treatment group (7.2%±0.9% vs 11.6%±1.2% vs 16.2%±2.1%, P<0.01). Compared with the control group, the number of apoptosis of endogenous cells in the EPC-APN transplantation group was significantly reduced (1.0%±0.1%/mm2 vs 2.1%±0.2%/mm2 vs 11.2%±3.2%/mm2, P<0.05). Mice in the EPC-APN transplant group had lower neurobehavioral mNSS scores (3.2±0.3 vs 4.5±0.3 vs 5.8±0.5, P<0.05). The number of angiogenesis in the brain of the EPC-APN transplantation group increased significantly[(6.2±0.8)/mm2 vs(1.8±0.2)/mm2 vs (0.2±0.1)/mm2, P<0.01]. Western blot analysis showed that the VEGF expression in EPC-APN group was up-regulated compared with EPC-GFP group (P<0.05). Conclusions: EPC-APN transplantation can reduce ischemic brain injury and improve neurobehavioral outcomes. |
| first_indexed | 2024-04-09T16:17:50Z |
| format | Article |
| id | doaj.art-20717eaac7c04055a0e5369e9b866d6b |
| institution | Directory Open Access Journal |
| issn | 1671-2870 |
| language | zho |
| last_indexed | 2024-04-09T16:17:50Z |
| publishDate | 2022-12-01 |
| publisher | Editorial Office of Journal of Diagnostics Concepts & Practice |
| record_format | Article |
| series | Zhenduanxue lilun yu shijian |
| spelling | doaj.art-20717eaac7c04055a0e5369e9b866d6b2023-04-24T01:14:10ZzhoEditorial Office of Journal of Diagnostics Concepts & PracticeZhenduanxue lilun yu shijian1671-28702022-12-01210669169610.16150/j.1671-2870.2022.06.005Transplantation of adiponectin-transduced endothelial progenitor cells improves neurobehavioral outcomes following transient middle cerebral artery occlusionSHEN Linhui, WANG Shuhong, MIAO Jie01. Department of Geriatrics, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China;2. Department of Neurology, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200123, ChinaObjective: To observe the effect of APN-modified EPCs on brain tissue repair and neurological function recovery in ischemic stroke model mice. Methods: Construction of lentiviruses transfected with APN genes. Genetically modified EPCs overexpressing APN(EPC-APN) or green fluorescent protein (EPC-GFP) were transplanted into a mice model of transient middle cerebral artery occlusion (tMCAO). The EPC-APN transplantation group, EPC-green fluorescent protein (GFP) transplantation control group and phosphate buffer (PBS) control treatment group were established. After transplantation, the enhanced GFP-expressing cells were found at the peri-infact area of brain. At 1, 7 and 14 days after tMCAO, the infarct volume was measured by using cresyl violet. Angiogenesis in the perifocal region was quantified on the 14th day. The apoptosis of endogenous cells was evaluated using TUNEL assay. Expression of vascular endothelial growth factor（VEGF）was detected using Western blot. Results: By constructing APN gene lentiviral vectors to transfect to EPCs, EPCs overexpressing APN were successfully prepared. The volume of brain atrophy in the EPC-APN transplantation group on on the 14th day after cerebral ischemia was significantly reduced compared with that in the EPC-GFP transplantation control group and the PBS control treatment group (7.2%±0.9% vs 11.6%±1.2% vs 16.2%±2.1%, P<0.01). Compared with the control group, the number of apoptosis of endogenous cells in the EPC-APN transplantation group was significantly reduced (1.0%±0.1%/mm2 vs 2.1%±0.2%/mm2 vs 11.2%±3.2%/mm2, P<0.05). Mice in the EPC-APN transplant group had lower neurobehavioral mNSS scores (3.2±0.3 vs 4.5±0.3 vs 5.8±0.5, P<0.05). The number of angiogenesis in the brain of the EPC-APN transplantation group increased significantly[(6.2±0.8)/mm2 vs(1.8±0.2)/mm2 vs (0.2±0.1)/mm2, P<0.01]. Western blot analysis showed that the VEGF expression in EPC-APN group was up-regulated compared with EPC-GFP group (P<0.05). Conclusions: EPC-APN transplantation can reduce ischemic brain injury and improve neurobehavioral outcomes.https://www.qk.sjtu.edu.cn/jdcp/fileup/1671-2870/PDF/1682220171051-753291557.pdf|adiponectin|end othelial progenitor cell|ischemic stroke|angiogenesis |
| spellingShingle | SHEN Linhui, WANG Shuhong, MIAO Jie Transplantation of adiponectin-transduced endothelial progenitor cells improves neurobehavioral outcomes following transient middle cerebral artery occlusion Zhenduanxue lilun yu shijian |adiponectin|end othelial progenitor cell|ischemic stroke|angiogenesis |
| title | Transplantation of adiponectin-transduced endothelial progenitor cells improves neurobehavioral outcomes following transient middle cerebral artery occlusion |
| title_full | Transplantation of adiponectin-transduced endothelial progenitor cells improves neurobehavioral outcomes following transient middle cerebral artery occlusion |
| title_fullStr | Transplantation of adiponectin-transduced endothelial progenitor cells improves neurobehavioral outcomes following transient middle cerebral artery occlusion |
| title_full_unstemmed | Transplantation of adiponectin-transduced endothelial progenitor cells improves neurobehavioral outcomes following transient middle cerebral artery occlusion |
| title_short | Transplantation of adiponectin-transduced endothelial progenitor cells improves neurobehavioral outcomes following transient middle cerebral artery occlusion |
| title_sort | transplantation of adiponectin transduced endothelial progenitor cells improves neurobehavioral outcomes following transient middle cerebral artery occlusion |
| topic | |adiponectin|end othelial progenitor cell|ischemic stroke|angiogenesis |
| url | https://www.qk.sjtu.edu.cn/jdcp/fileup/1671-2870/PDF/1682220171051-753291557.pdf |
| work_keys_str_mv | AT shenlinhuiwangshuhongmiaojie transplantationofadiponectintransducedendothelialprogenitorcellsimprovesneurobehavioraloutcomesfollowingtransientmiddlecerebralarteryocclusion |