Intra-Amniotic Administration of Cashew Nut (<i>Anacardium occidentale</i> L.) Soluble Extract Improved Gut Functionality and Morphology In Vivo (<i>Gallus gallus</i>)
Cashew nuts are rich in dietary fibers, monounsaturated fatty acids, carotenoids, tocopherols, flavonoids, catechins, amino acids, and minerals that offer benefits for health. However, the knowledge of its effect on gut health is lacking. In this way, cashew nut soluble extract (CNSE) was assessed i...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/15/10/2378 |
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| author | Talitha Silva Meneguelli Nikolai Kolba Arundhati Misra Ana Paula Dionísio Ana Claudia Pelissari Kravchychyn Bárbara Pereira Da Silva Hercia Stampini Duarte Martino Helen Hermana Miranda Hermsdorff Elad Tako |
| author_facet | Talitha Silva Meneguelli Nikolai Kolba Arundhati Misra Ana Paula Dionísio Ana Claudia Pelissari Kravchychyn Bárbara Pereira Da Silva Hercia Stampini Duarte Martino Helen Hermana Miranda Hermsdorff Elad Tako |
| author_sort | Talitha Silva Meneguelli |
| collection | DOAJ |
| description | Cashew nuts are rich in dietary fibers, monounsaturated fatty acids, carotenoids, tocopherols, flavonoids, catechins, amino acids, and minerals that offer benefits for health. However, the knowledge of its effect on gut health is lacking. In this way, cashew nut soluble extract (CNSE) was assessed in vivo via intra-amniotic administration in intestinal brush border membrane (BBM) morphology, functionality, and gut microbiota. Four groups were evaluated: (1) no injection (control); (2) H<sub>2</sub>O injection (control); (3) 10 mg/mL CNSE (1%); and (4) 50 mg/mL CNSE (5%). Results related to CNSE on duodenal morphological parameters showed higher Paneth cell numbers, goblet cell (GC) diameter in crypt and villi, depth crypt, mixed GC per villi, and villi surface area. Further, it decreased GC number and acid and neutral GC. In the gut microbiota, treatment with CNSE showed a lower abundance of <i>Bifidobacterium</i>, <i>Lactobacillus</i>, and <i>E. coli</i>. Further, in intestinal functionality, CNSE upregulated aminopeptidase (AP) gene expression at 5% compared to 1% CNSE. In conclusion, CNSE had beneficial effects on gut health by improving duodenal BBM functionality, as it upregulated AP gene expression, and by modifying morphological parameters ameliorating digestive and absorptive capacity. For intestinal microbiota, higher concentrations of CNSE or long-term intervention may be necessary. |
| first_indexed | 2024-03-11T03:25:10Z |
| format | Article |
| id | doaj.art-207715f7bf5c49ecbf6be507341216c7 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-11T03:25:10Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-207715f7bf5c49ecbf6be507341216c72023-11-18T02:45:33ZengMDPI AGNutrients2072-66432023-05-011510237810.3390/nu15102378Intra-Amniotic Administration of Cashew Nut (<i>Anacardium occidentale</i> L.) Soluble Extract Improved Gut Functionality and Morphology In Vivo (<i>Gallus gallus</i>)Talitha Silva Meneguelli0Nikolai Kolba1Arundhati Misra2Ana Paula Dionísio3Ana Claudia Pelissari Kravchychyn4Bárbara Pereira Da Silva5Hercia Stampini Duarte Martino6Helen Hermana Miranda Hermsdorff7Elad Tako8Department of Food Science, Cornell University, Ithaca, NY 14850, USADepartment of Food Science, Cornell University, Ithaca, NY 14850, USADepartment of Food Science, Cornell University, Ithaca, NY 14850, USAEmbrapa Agroindústria Tropical—CNPAT, Fortaleza 60511-110, CE, BrazilDepartment of Nutrition and Health, Universidade Federal de Vicosa, Viçosa 36570-900, MG, BrazilDepartment of Nutrition and Health, Universidade Federal de Vicosa, Viçosa 36570-900, MG, BrazilDepartment of Nutrition and Health, Universidade Federal de Vicosa, Viçosa 36570-900, MG, BrazilDepartment of Nutrition and Health, Universidade Federal de Vicosa, Viçosa 36570-900, MG, BrazilDepartment of Food Science, Cornell University, Ithaca, NY 14850, USACashew nuts are rich in dietary fibers, monounsaturated fatty acids, carotenoids, tocopherols, flavonoids, catechins, amino acids, and minerals that offer benefits for health. However, the knowledge of its effect on gut health is lacking. In this way, cashew nut soluble extract (CNSE) was assessed in vivo via intra-amniotic administration in intestinal brush border membrane (BBM) morphology, functionality, and gut microbiota. Four groups were evaluated: (1) no injection (control); (2) H<sub>2</sub>O injection (control); (3) 10 mg/mL CNSE (1%); and (4) 50 mg/mL CNSE (5%). Results related to CNSE on duodenal morphological parameters showed higher Paneth cell numbers, goblet cell (GC) diameter in crypt and villi, depth crypt, mixed GC per villi, and villi surface area. Further, it decreased GC number and acid and neutral GC. In the gut microbiota, treatment with CNSE showed a lower abundance of <i>Bifidobacterium</i>, <i>Lactobacillus</i>, and <i>E. coli</i>. Further, in intestinal functionality, CNSE upregulated aminopeptidase (AP) gene expression at 5% compared to 1% CNSE. In conclusion, CNSE had beneficial effects on gut health by improving duodenal BBM functionality, as it upregulated AP gene expression, and by modifying morphological parameters ameliorating digestive and absorptive capacity. For intestinal microbiota, higher concentrations of CNSE or long-term intervention may be necessary.https://www.mdpi.com/2072-6643/15/10/2378cashew nut<i>Anacardium occidentale</i> L.<i>Gallus gallus</i>gut health |
| spellingShingle | Talitha Silva Meneguelli Nikolai Kolba Arundhati Misra Ana Paula Dionísio Ana Claudia Pelissari Kravchychyn Bárbara Pereira Da Silva Hercia Stampini Duarte Martino Helen Hermana Miranda Hermsdorff Elad Tako Intra-Amniotic Administration of Cashew Nut (<i>Anacardium occidentale</i> L.) Soluble Extract Improved Gut Functionality and Morphology In Vivo (<i>Gallus gallus</i>) Nutrients cashew nut <i>Anacardium occidentale</i> L. <i>Gallus gallus</i> gut health |
| title | Intra-Amniotic Administration of Cashew Nut (<i>Anacardium occidentale</i> L.) Soluble Extract Improved Gut Functionality and Morphology In Vivo (<i>Gallus gallus</i>) |
| title_full | Intra-Amniotic Administration of Cashew Nut (<i>Anacardium occidentale</i> L.) Soluble Extract Improved Gut Functionality and Morphology In Vivo (<i>Gallus gallus</i>) |
| title_fullStr | Intra-Amniotic Administration of Cashew Nut (<i>Anacardium occidentale</i> L.) Soluble Extract Improved Gut Functionality and Morphology In Vivo (<i>Gallus gallus</i>) |
| title_full_unstemmed | Intra-Amniotic Administration of Cashew Nut (<i>Anacardium occidentale</i> L.) Soluble Extract Improved Gut Functionality and Morphology In Vivo (<i>Gallus gallus</i>) |
| title_short | Intra-Amniotic Administration of Cashew Nut (<i>Anacardium occidentale</i> L.) Soluble Extract Improved Gut Functionality and Morphology In Vivo (<i>Gallus gallus</i>) |
| title_sort | intra amniotic administration of cashew nut i anacardium occidentale i l soluble extract improved gut functionality and morphology in vivo i gallus gallus i |
| topic | cashew nut <i>Anacardium occidentale</i> L. <i>Gallus gallus</i> gut health |
| url | https://www.mdpi.com/2072-6643/15/10/2378 |
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