Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of China
Abstract Background Enterobacter cloacae complex (ECC) is one of the most common extended-spectrum β-lactamase and carbapenemase-producing pathogen that threatens millions of the elderly and vulnerable sick persons. The objective of this study was to perform the molecular characteristics of the carb...
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| Format: | Article |
| Language: | English |
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BMC
2020-01-01
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| Series: | BMC Infectious Diseases |
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| Online Access: | https://doi.org/10.1186/s12879-020-4768-7 |
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| author | Yongxin Zhao Jisheng Zhang Yanjun Fu Chunjiang Li Kewang Hu Shanshan Su Lan Yu Yuhang Guo Yu Fu Xiaoli Zhang |
| author_facet | Yongxin Zhao Jisheng Zhang Yanjun Fu Chunjiang Li Kewang Hu Shanshan Su Lan Yu Yuhang Guo Yu Fu Xiaoli Zhang |
| author_sort | Yongxin Zhao |
| collection | DOAJ |
| description | Abstract Background Enterobacter cloacae complex (ECC) is one of the most common extended-spectrum β-lactamase and carbapenemase-producing pathogen that threatens millions of the elderly and vulnerable sick persons. The objective of this study was to perform the molecular characteristics of the carbapenem-resistant E. cloacae complex (CREC) emerged in Heilongjiang Province of China. Methods Six CREC strains were isolated from the patients with infectious diseases. The identities of ECC isolates were confirmed by sequencing the polymerase chain reaction (PCR) products of 16S rRNA gene. The characterization of the CREC isolates were analyzed by sequencing PCR products of the carbapenemase, ampC and fluoroquinolone resistance genes and performing multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) and whole genome sequencing. Results All 6 isolates harbored multiple resistance genes. Of them, 5 carried metallo-β-lactamases and one was bla KPC-2-positive. The levofloxacin and ciprofloxacin-resistant strains had substitutions of gyrA83, gyrA87, and parC80 in the quinolone-resistance determining regions. The MLST analyses revealed that 6 isolates belonged to five sequence types (ST520, ST528, ST1119, ST1120, and ST93) while the PFGE patterns of the isolates fallen into four clusters. The strain ST1120 was found to carry two separated plasmids that encode bla NDM-1 and bla IMP-4. Conclusions Our study, for the first time, identified a CREC strain that co-produces bla NDM-1 and bla IMP-4 in the Northeast China. Our finding emphasizes an urgent need for more intensive surveillance and precaution measures to prevent the CERC spread. |
| first_indexed | 2024-12-20T05:08:33Z |
| format | Article |
| id | doaj.art-2078204a68084fe384e8653bd3b75055 |
| institution | Directory Open Access Journal |
| issn | 1471-2334 |
| language | English |
| last_indexed | 2024-12-20T05:08:33Z |
| publishDate | 2020-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Infectious Diseases |
| spelling | doaj.art-2078204a68084fe384e8653bd3b750552022-12-21T19:52:18ZengBMCBMC Infectious Diseases1471-23342020-01-012011710.1186/s12879-020-4768-7Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of ChinaYongxin Zhao0Jisheng Zhang1Yanjun Fu2Chunjiang Li3Kewang Hu4Shanshan Su5Lan Yu6Yuhang Guo7Yu Fu8Xiaoli Zhang9Yongchuan Hospital of Chongqing Medical UniversityYongchuan Hospital of Chongqing Medical Universitythe First Affiliated Hospital of Jiamusi UniversityJiamusi Universitythe First Affiliated Hospital of Jiamusi Universitythe First Affiliated Hospital of Jiamusi Universitythe First Affiliated Hospital of Jiamusi Universitythe First Affiliated Hospital of Jiamusi UniversityCenter for Disease Control and PreventionYongchuan Hospital of Chongqing Medical UniversityAbstract Background Enterobacter cloacae complex (ECC) is one of the most common extended-spectrum β-lactamase and carbapenemase-producing pathogen that threatens millions of the elderly and vulnerable sick persons. The objective of this study was to perform the molecular characteristics of the carbapenem-resistant E. cloacae complex (CREC) emerged in Heilongjiang Province of China. Methods Six CREC strains were isolated from the patients with infectious diseases. The identities of ECC isolates were confirmed by sequencing the polymerase chain reaction (PCR) products of 16S rRNA gene. The characterization of the CREC isolates were analyzed by sequencing PCR products of the carbapenemase, ampC and fluoroquinolone resistance genes and performing multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) and whole genome sequencing. Results All 6 isolates harbored multiple resistance genes. Of them, 5 carried metallo-β-lactamases and one was bla KPC-2-positive. The levofloxacin and ciprofloxacin-resistant strains had substitutions of gyrA83, gyrA87, and parC80 in the quinolone-resistance determining regions. The MLST analyses revealed that 6 isolates belonged to five sequence types (ST520, ST528, ST1119, ST1120, and ST93) while the PFGE patterns of the isolates fallen into four clusters. The strain ST1120 was found to carry two separated plasmids that encode bla NDM-1 and bla IMP-4. Conclusions Our study, for the first time, identified a CREC strain that co-produces bla NDM-1 and bla IMP-4 in the Northeast China. Our finding emphasizes an urgent need for more intensive surveillance and precaution measures to prevent the CERC spread.https://doi.org/10.1186/s12879-020-4768-7Enterobacter cloacae complexMetallo-β-lactamaseFluoroquinolone resistanceST93CREC |
| spellingShingle | Yongxin Zhao Jisheng Zhang Yanjun Fu Chunjiang Li Kewang Hu Shanshan Su Lan Yu Yuhang Guo Yu Fu Xiaoli Zhang Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of China BMC Infectious Diseases Enterobacter cloacae complex Metallo-β-lactamase Fluoroquinolone resistance ST93 CREC |
| title | Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of China |
| title_full | Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of China |
| title_fullStr | Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of China |
| title_full_unstemmed | Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of China |
| title_short | Molecular characterization of metallo-β-lactamase- producing carbapenem-resistant Enterobacter cloacae complex isolated in Heilongjiang Province of China |
| title_sort | molecular characterization of metallo β lactamase producing carbapenem resistant enterobacter cloacae complex isolated in heilongjiang province of china |
| topic | Enterobacter cloacae complex Metallo-β-lactamase Fluoroquinolone resistance ST93 CREC |
| url | https://doi.org/10.1186/s12879-020-4768-7 |
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