<sup>1</sup>H-NMR-Based Analysis for Exploring Knee Synovial Fluid Metabolite Changes after Local Cryotherapy in Knee Arthritis Patients
Rehabilitation using cryotherapy has widely been used in inflammatory diseases to relieve pain and decrease the disease activity. The aim of this study was to explore the metabolite changes in inflammatory knee-joint synovial fluids following local cryotherapy treatment (ice or cold CO<sub>2&l...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-11-01
|
| Series: | Metabolites |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2218-1989/10/11/460 |
| _version_ | 1797547962439565312 |
|---|---|
| author | Wafa Douzi Xavier Guillot Delphine Bon François Seguin Nadège Boildieu Daniel Wendling Nicolas Tordi Olivier Dupuy Benoit Dugué |
| author_facet | Wafa Douzi Xavier Guillot Delphine Bon François Seguin Nadège Boildieu Daniel Wendling Nicolas Tordi Olivier Dupuy Benoit Dugué |
| author_sort | Wafa Douzi |
| collection | DOAJ |
| description | Rehabilitation using cryotherapy has widely been used in inflammatory diseases to relieve pain and decrease the disease activity. The aim of this study was to explore the metabolite changes in inflammatory knee-joint synovial fluids following local cryotherapy treatment (ice or cold CO<sub>2</sub>). We used proton nuclear magnetic resonance (<sup>1</sup>H NMR) spectroscopy to assess the metabolite patterns in synovial fluid (SF) in patients with knee arthritis (<i>n</i> = 46) before (D0) and after (D1, 24 h later) two applications of local cryotherapy. Spectra from aqueous samples and organic extracts were obtained with an 11.75 Tesla spectrometer. The metabolite concentrations within the SF were compared between D1 and D0 using multiple comparisons with the application of a false discovery rate (FDR) adjusted at 10% for each metabolite. A total of 32 metabolites/chemical structures were identified including amino acids, organic acids, fatty acids or sugars. Pyruvate, alanine, citrate, threonine was significantly higher at D1 vs D0 (<i>p</i> < 0.05). Tyrosine concentration significantly decreases after cryotherapy application (<i>p</i> < 0.001). We did not observe any effect of gender and cooling technique on metabolite concentrations between D0 and D1 (<i>p</i> > 0.05). The present study provides new insight into a short-term effect of cold stimulus in synovial fluid from patients with knee arthritis. Our observations suggest that the increased level of metabolites involved in energy metabolism may explain the underlying molecular pathways that mediate the antioxidant and anti-inflammatory capacities of cryotherapy. |
| first_indexed | 2024-03-10T14:52:35Z |
| format | Article |
| id | doaj.art-2078b8a1fb28436a94a85db4b5c0c40a |
| institution | Directory Open Access Journal |
| issn | 2218-1989 |
| language | English |
| last_indexed | 2024-03-10T14:52:35Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj.art-2078b8a1fb28436a94a85db4b5c0c40a2023-11-20T20:52:26ZengMDPI AGMetabolites2218-19892020-11-01101146010.3390/metabo10110460<sup>1</sup>H-NMR-Based Analysis for Exploring Knee Synovial Fluid Metabolite Changes after Local Cryotherapy in Knee Arthritis PatientsWafa Douzi0Xavier Guillot1Delphine Bon2François Seguin3Nadège Boildieu4Daniel Wendling5Nicolas Tordi6Olivier Dupuy7Benoit Dugué8Laboratoire «Mobilité, Vieillissement, Exercice (MOVE)–EA6314», Faculté des Sciences du Sport, Université de Poitiers, 8 Allée Jean Monnet, 86000 Poitiers, FranceDepartment of Rheumatology, Felix Guyon University Hospital, 97400 Saint-Denis, FranceINSERM U1082, (IRTOMIT), Poitiers, France and Faculty of Medicine and Pharmacy, University of Poitiers, 86000 Poitiers, FranceINSERM U1082, (IRTOMIT), Poitiers, France and Faculty of Medicine and Pharmacy, University of Poitiers, 86000 Poitiers, FranceINSERM U1082, (IRTOMIT), Poitiers, France and Faculty of Medicine and Pharmacy, University of Poitiers, 86000 Poitiers, FranceDepartment of Rheumatology, CHRU de Besançon, Boulevard Fleming, F-25030 Besançon, FrancePEPITE EA4267, (EPSI), University Bourgogne Franche-Comté, F-25000 Besançon, FranceLaboratoire «Mobilité, Vieillissement, Exercice (MOVE)–EA6314», Faculté des Sciences du Sport, Université de Poitiers, 8 Allée Jean Monnet, 86000 Poitiers, FranceLaboratoire «Mobilité, Vieillissement, Exercice (MOVE)–EA6314», Faculté des Sciences du Sport, Université de Poitiers, 8 Allée Jean Monnet, 86000 Poitiers, FranceRehabilitation using cryotherapy has widely been used in inflammatory diseases to relieve pain and decrease the disease activity. The aim of this study was to explore the metabolite changes in inflammatory knee-joint synovial fluids following local cryotherapy treatment (ice or cold CO<sub>2</sub>). We used proton nuclear magnetic resonance (<sup>1</sup>H NMR) spectroscopy to assess the metabolite patterns in synovial fluid (SF) in patients with knee arthritis (<i>n</i> = 46) before (D0) and after (D1, 24 h later) two applications of local cryotherapy. Spectra from aqueous samples and organic extracts were obtained with an 11.75 Tesla spectrometer. The metabolite concentrations within the SF were compared between D1 and D0 using multiple comparisons with the application of a false discovery rate (FDR) adjusted at 10% for each metabolite. A total of 32 metabolites/chemical structures were identified including amino acids, organic acids, fatty acids or sugars. Pyruvate, alanine, citrate, threonine was significantly higher at D1 vs D0 (<i>p</i> < 0.05). Tyrosine concentration significantly decreases after cryotherapy application (<i>p</i> < 0.001). We did not observe any effect of gender and cooling technique on metabolite concentrations between D0 and D1 (<i>p</i> > 0.05). The present study provides new insight into a short-term effect of cold stimulus in synovial fluid from patients with knee arthritis. Our observations suggest that the increased level of metabolites involved in energy metabolism may explain the underlying molecular pathways that mediate the antioxidant and anti-inflammatory capacities of cryotherapy.https://www.mdpi.com/2218-1989/10/11/460metabolitesnuclear magnetic resonance spectroscopycryotherapysynovial inflammationarthritis |
| spellingShingle | Wafa Douzi Xavier Guillot Delphine Bon François Seguin Nadège Boildieu Daniel Wendling Nicolas Tordi Olivier Dupuy Benoit Dugué <sup>1</sup>H-NMR-Based Analysis for Exploring Knee Synovial Fluid Metabolite Changes after Local Cryotherapy in Knee Arthritis Patients Metabolites metabolites nuclear magnetic resonance spectroscopy cryotherapy synovial inflammation arthritis |
| title | <sup>1</sup>H-NMR-Based Analysis for Exploring Knee Synovial Fluid Metabolite Changes after Local Cryotherapy in Knee Arthritis Patients |
| title_full | <sup>1</sup>H-NMR-Based Analysis for Exploring Knee Synovial Fluid Metabolite Changes after Local Cryotherapy in Knee Arthritis Patients |
| title_fullStr | <sup>1</sup>H-NMR-Based Analysis for Exploring Knee Synovial Fluid Metabolite Changes after Local Cryotherapy in Knee Arthritis Patients |
| title_full_unstemmed | <sup>1</sup>H-NMR-Based Analysis for Exploring Knee Synovial Fluid Metabolite Changes after Local Cryotherapy in Knee Arthritis Patients |
| title_short | <sup>1</sup>H-NMR-Based Analysis for Exploring Knee Synovial Fluid Metabolite Changes after Local Cryotherapy in Knee Arthritis Patients |
| title_sort | sup 1 sup h nmr based analysis for exploring knee synovial fluid metabolite changes after local cryotherapy in knee arthritis patients |
| topic | metabolites nuclear magnetic resonance spectroscopy cryotherapy synovial inflammation arthritis |
| url | https://www.mdpi.com/2218-1989/10/11/460 |
| work_keys_str_mv | AT wafadouzi sup1suphnmrbasedanalysisforexploringkneesynovialfluidmetabolitechangesafterlocalcryotherapyinkneearthritispatients AT xavierguillot sup1suphnmrbasedanalysisforexploringkneesynovialfluidmetabolitechangesafterlocalcryotherapyinkneearthritispatients AT delphinebon sup1suphnmrbasedanalysisforexploringkneesynovialfluidmetabolitechangesafterlocalcryotherapyinkneearthritispatients AT francoisseguin sup1suphnmrbasedanalysisforexploringkneesynovialfluidmetabolitechangesafterlocalcryotherapyinkneearthritispatients AT nadegeboildieu sup1suphnmrbasedanalysisforexploringkneesynovialfluidmetabolitechangesafterlocalcryotherapyinkneearthritispatients AT danielwendling sup1suphnmrbasedanalysisforexploringkneesynovialfluidmetabolitechangesafterlocalcryotherapyinkneearthritispatients AT nicolastordi sup1suphnmrbasedanalysisforexploringkneesynovialfluidmetabolitechangesafterlocalcryotherapyinkneearthritispatients AT olivierdupuy sup1suphnmrbasedanalysisforexploringkneesynovialfluidmetabolitechangesafterlocalcryotherapyinkneearthritispatients AT benoitdugue sup1suphnmrbasedanalysisforexploringkneesynovialfluidmetabolitechangesafterlocalcryotherapyinkneearthritispatients |