FORK-seq: replication landscape of the Saccharomyces cerevisiae genome by nanopore sequencing

FORK-seq: replication landscape of the Saccharomyces cerevisiae genome by nanopore sequencing

Abstract Genome replication mapping methods profile cell populations, masking cell-to-cell heterogeneity. Here, we describe FORK-seq, a nanopore sequencing method to map replication of single DNA molecules at 200-nucleotide resolution. By quantifying BrdU incorporation along pulse-chased replication...

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Bibliographic Details
Main Authors: Magali Hennion, Jean-Michel Arbona, Laurent Lacroix, Corinne Cruaud, Bertrand Theulot, Benoît Le Tallec, Florence Proux, Xia Wu, Elizaveta Novikova, Stefan Engelen, Arnaud Lemainque, Benjamin Audit, Olivier Hyrien
Format: Article
Language:English
Published: BMC 2020-05-01
Series:Genome Biology
Subjects:
DNA replication
Nanopore sequencing
Bromodeoxyuridine
Single-molecule analysis
S. cerevisiae
Convolutional neural network
Online Access:http://link.springer.com/article/10.1186/s13059-020-02013-3

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