Evaluation of the CRISPR/Cas9 Genetic Constructs in Efficient Disruption of Porcine Genes for Xenotransplantation Purposes Along with an Assessment of the Off-Target Mutation Formation

Evaluation of the CRISPR/Cas9 Genetic Constructs in Efficient Disruption of Porcine Genes for Xenotransplantation Purposes Along with an Assessment of the Off-Target Mutation Formation

The increasing life expectancy of humans has led to an increase in the number of patients with chronic diseases and organ failure. However, the imbalance between the supply and the demand for human organs is a serious problem in modern transplantology. One of many solutions to overcome this problem...

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Bibliographic Details
Main Authors: Natalia Ryczek, Magdalena Hryhorowicz, Daniel Lipiński, Joanna Zeyland, Ryszard Słomski
Format: Article
Language:English
Published: MDPI AG 2020-06-01
Series:Genes
Subjects:
xenoantigen
coagulation system dysregulation
CRISPR/Cas9 system
genome modifications
non-homologous DNA ends joining (NHEJ)
TIDE analysis
Online Access:https://www.mdpi.com/2073-4425/11/6/713

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