Computational and experimental analysis of the secretome of Methylococcus capsulatus (Bath).
The Gram-negative methanotroph Methylococcus capsulatus (Bath) was recently demonstrated to abrogate inflammation in a murine model of inflammatory bowel disease, suggesting interactions with cells involved in maintaining mucosal homeostasis and emphasizing the importance of understanding the many p...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4257694?pdf=render |
| _version_ | 1818673051931246592 |
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| author | Stine Indrelid Geir Mathiesen Morten Jacobsen Tor Lea Charlotte R Kleiveland |
| author_facet | Stine Indrelid Geir Mathiesen Morten Jacobsen Tor Lea Charlotte R Kleiveland |
| author_sort | Stine Indrelid |
| collection | DOAJ |
| description | The Gram-negative methanotroph Methylococcus capsulatus (Bath) was recently demonstrated to abrogate inflammation in a murine model of inflammatory bowel disease, suggesting interactions with cells involved in maintaining mucosal homeostasis and emphasizing the importance of understanding the many properties of M. capsulatus. Secreted proteins determine how bacteria may interact with their environment, and a comprehensive knowledge of such proteins is therefore vital to understand bacterial physiology and behavior. The aim of this study was to systematically analyze protein secretion in M. capsulatus (Bath) by identifying the secretion systems present and the respective secreted substrates. Computational analysis revealed that in addition to previously recognized type II secretion systems and a type VII secretion system, a type Vb (two-partner) secretion system and putative type I secretion systems are present in M. capsulatus (Bath). In silico analysis suggests that the diverse secretion systems in M.capsulatus transport proteins likely to be involved in adhesion, colonization, nutrient acquisition and homeostasis maintenance. Results of the computational analysis was verified and extended by an experimental approach showing that in addition an uncharacterized protein and putative moonlighting proteins are released to the medium during exponential growth of M. capsulatus (Bath). |
| first_indexed | 2024-12-17T07:49:39Z |
| format | Article |
| id | doaj.art-207e04c7144c49bab0007062ed3309a1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-17T07:49:39Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-207e04c7144c49bab0007062ed3309a12022-12-21T21:57:53ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-01912e11447610.1371/journal.pone.0114476Computational and experimental analysis of the secretome of Methylococcus capsulatus (Bath).Stine IndrelidGeir MathiesenMorten JacobsenTor LeaCharlotte R KleivelandThe Gram-negative methanotroph Methylococcus capsulatus (Bath) was recently demonstrated to abrogate inflammation in a murine model of inflammatory bowel disease, suggesting interactions with cells involved in maintaining mucosal homeostasis and emphasizing the importance of understanding the many properties of M. capsulatus. Secreted proteins determine how bacteria may interact with their environment, and a comprehensive knowledge of such proteins is therefore vital to understand bacterial physiology and behavior. The aim of this study was to systematically analyze protein secretion in M. capsulatus (Bath) by identifying the secretion systems present and the respective secreted substrates. Computational analysis revealed that in addition to previously recognized type II secretion systems and a type VII secretion system, a type Vb (two-partner) secretion system and putative type I secretion systems are present in M. capsulatus (Bath). In silico analysis suggests that the diverse secretion systems in M.capsulatus transport proteins likely to be involved in adhesion, colonization, nutrient acquisition and homeostasis maintenance. Results of the computational analysis was verified and extended by an experimental approach showing that in addition an uncharacterized protein and putative moonlighting proteins are released to the medium during exponential growth of M. capsulatus (Bath).http://europepmc.org/articles/PMC4257694?pdf=render |
| spellingShingle | Stine Indrelid Geir Mathiesen Morten Jacobsen Tor Lea Charlotte R Kleiveland Computational and experimental analysis of the secretome of Methylococcus capsulatus (Bath). PLoS ONE |
| title | Computational and experimental analysis of the secretome of Methylococcus capsulatus (Bath). |
| title_full | Computational and experimental analysis of the secretome of Methylococcus capsulatus (Bath). |
| title_fullStr | Computational and experimental analysis of the secretome of Methylococcus capsulatus (Bath). |
| title_full_unstemmed | Computational and experimental analysis of the secretome of Methylococcus capsulatus (Bath). |
| title_short | Computational and experimental analysis of the secretome of Methylococcus capsulatus (Bath). |
| title_sort | computational and experimental analysis of the secretome of methylococcus capsulatus bath |
| url | http://europepmc.org/articles/PMC4257694?pdf=render |
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