Bioprospecting of <i>Helichrysum</i> Species: Chemical Profile, Phytochemical Properties, and Antifungal Efficacy against <i>Botrytis cinerea</i>
Variation in plant species and extraction solvents play a crucial role in the recovery of their bioactive compounds and antifungal efficacy. Thus, in this study, a comparative investigation was carried out using extraction solvents: 70% acetone and 95% ethanol to obtain crude aqueous extracts from &...
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| Format: | Article |
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MDPI AG
2022-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/1/58 |
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| author | Neliswa A Matrose Zinash A Belay Kenechukwu Obikeze Lucky Mokwena Oluwafemi James Caleb |
| author_facet | Neliswa A Matrose Zinash A Belay Kenechukwu Obikeze Lucky Mokwena Oluwafemi James Caleb |
| author_sort | Neliswa A Matrose |
| collection | DOAJ |
| description | Variation in plant species and extraction solvents play a crucial role in the recovery of their bioactive compounds and antifungal efficacy. Thus, in this study, a comparative investigation was carried out using extraction solvents: 70% acetone and 95% ethanol to obtain crude aqueous extracts from <i>Helichrysum odoratissimum</i> and <i>H. patulum</i>. Crude aqueous extracts were screened using gas chromatography–mass spectrometry (GC–MS), to gain insight into their chemical composition. Phytochemical properties (total polyphenols (TP) and radical scavenging capacity via 2,2-diphenyl-1-picrylhydrazyl (DPPH)), and antifungal activity against <i>Botrytis cinerea</i> of the crude extracts were evaluated. Fungicide (Rovral<sup>®</sup> WP) and extraction solvents were used as controls. Variation in <i>Helichrysum</i> spp. and extraction solvent had influence on the chemical composition, phytochemicals, and antifungal activities. Metabolites such as γ-terpinene (≈0.1%), α-amorphene (≈0.6%) α-gurjunene (≈1.4%), β-selinene (2.2–3.2%), γ-gurjunene (≈3.3%), and methyl cinnamate (≈20%) were detected only in extracts of <i>H. patulum</i>. Crude extract of <i>H. odoratissimum</i> using 70% acetone had the highest TP (19.3 ± 0.76 g GA 100 g<sup>−1</sup>), and DPPH capacity (13,251.5 ± 700.55 µmol Trolox g<sup>−1</sup>) compared to <i>H. patulum</i> (<i>p</i> ≤ 0.05). Ethanolic extracts of <i>H. patulum</i> showed highest antifungal efficacy (≈65%) against <i>B. cinerea</i> (<i>p</i> ≤ 0.05) compared to other crude extracts. This study showed that <i>Helichrysum</i> spp. differ in their potential as a source for bioactive compounds and antifungal treatments/formulations. |
| first_indexed | 2024-03-09T03:27:06Z |
| format | Article |
| id | doaj.art-207e52c82bb44f4f9ff8fd042201f482 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-09T03:27:06Z |
| publishDate | 2022-12-01 |
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| record_format | Article |
| series | Plants |
| spelling | doaj.art-207e52c82bb44f4f9ff8fd042201f4822023-12-03T15:00:16ZengMDPI AGPlants2223-77472022-12-011215810.3390/plants12010058Bioprospecting of <i>Helichrysum</i> Species: Chemical Profile, Phytochemical Properties, and Antifungal Efficacy against <i>Botrytis cinerea</i>Neliswa A Matrose0Zinash A Belay1Kenechukwu Obikeze2Lucky Mokwena3Oluwafemi James Caleb4Post-Harvest and Agro-Processing Technologies (PHATs), Agricultural Research Council (ARC) Infruitec-Nietvoorbij, Stellenbosch 7599, South AfricaPost-Harvest and Agro-Processing Technologies (PHATs), Agricultural Research Council (ARC) Infruitec-Nietvoorbij, Stellenbosch 7599, South AfricaSchool of Pharmacy, Faculty of Science, University of the Western Cape, Bellville 7535, South AfricaCentral Analytical Facility, Stellenbosch University, Matieland 7602, South AfricaDepartment of Food Science, Faculty of AgriSciences, Stellenbosch University, Matieland 7602, South AfricaVariation in plant species and extraction solvents play a crucial role in the recovery of their bioactive compounds and antifungal efficacy. Thus, in this study, a comparative investigation was carried out using extraction solvents: 70% acetone and 95% ethanol to obtain crude aqueous extracts from <i>Helichrysum odoratissimum</i> and <i>H. patulum</i>. Crude aqueous extracts were screened using gas chromatography–mass spectrometry (GC–MS), to gain insight into their chemical composition. Phytochemical properties (total polyphenols (TP) and radical scavenging capacity via 2,2-diphenyl-1-picrylhydrazyl (DPPH)), and antifungal activity against <i>Botrytis cinerea</i> of the crude extracts were evaluated. Fungicide (Rovral<sup>®</sup> WP) and extraction solvents were used as controls. Variation in <i>Helichrysum</i> spp. and extraction solvent had influence on the chemical composition, phytochemicals, and antifungal activities. Metabolites such as γ-terpinene (≈0.1%), α-amorphene (≈0.6%) α-gurjunene (≈1.4%), β-selinene (2.2–3.2%), γ-gurjunene (≈3.3%), and methyl cinnamate (≈20%) were detected only in extracts of <i>H. patulum</i>. Crude extract of <i>H. odoratissimum</i> using 70% acetone had the highest TP (19.3 ± 0.76 g GA 100 g<sup>−1</sup>), and DPPH capacity (13,251.5 ± 700.55 µmol Trolox g<sup>−1</sup>) compared to <i>H. patulum</i> (<i>p</i> ≤ 0.05). Ethanolic extracts of <i>H. patulum</i> showed highest antifungal efficacy (≈65%) against <i>B. cinerea</i> (<i>p</i> ≤ 0.05) compared to other crude extracts. This study showed that <i>Helichrysum</i> spp. differ in their potential as a source for bioactive compounds and antifungal treatments/formulations.https://www.mdpi.com/2223-7747/12/1/58antioxidant capacitycrude extractsextraction solvents‘imphepho’secondary metabolitessesquiterpenoids |
| spellingShingle | Neliswa A Matrose Zinash A Belay Kenechukwu Obikeze Lucky Mokwena Oluwafemi James Caleb Bioprospecting of <i>Helichrysum</i> Species: Chemical Profile, Phytochemical Properties, and Antifungal Efficacy against <i>Botrytis cinerea</i> Plants antioxidant capacity crude extracts extraction solvents ‘imphepho’ secondary metabolites sesquiterpenoids |
| title | Bioprospecting of <i>Helichrysum</i> Species: Chemical Profile, Phytochemical Properties, and Antifungal Efficacy against <i>Botrytis cinerea</i> |
| title_full | Bioprospecting of <i>Helichrysum</i> Species: Chemical Profile, Phytochemical Properties, and Antifungal Efficacy against <i>Botrytis cinerea</i> |
| title_fullStr | Bioprospecting of <i>Helichrysum</i> Species: Chemical Profile, Phytochemical Properties, and Antifungal Efficacy against <i>Botrytis cinerea</i> |
| title_full_unstemmed | Bioprospecting of <i>Helichrysum</i> Species: Chemical Profile, Phytochemical Properties, and Antifungal Efficacy against <i>Botrytis cinerea</i> |
| title_short | Bioprospecting of <i>Helichrysum</i> Species: Chemical Profile, Phytochemical Properties, and Antifungal Efficacy against <i>Botrytis cinerea</i> |
| title_sort | bioprospecting of i helichrysum i species chemical profile phytochemical properties and antifungal efficacy against i botrytis cinerea i |
| topic | antioxidant capacity crude extracts extraction solvents ‘imphepho’ secondary metabolites sesquiterpenoids |
| url | https://www.mdpi.com/2223-7747/12/1/58 |
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