Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay
Escherichia coli O157:H7 is regarded as one of the most harmful pathogenic microorganisms related to foodborne diseases. This paper proposes a rapid-detection biosensor for the sensitive and quantitative analysis of E. coli O157:H7 in biological samples by surface-enhanced Raman scattering (SERS)-ba...
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Frontiers Media S.A.
2020-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2020.596005/full |
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| author | Luoluo Shi Luoluo Shi Luoluo Shi Ling Xu Ling Xu Rui Xiao Zihui Zhou Chongwen Wang Chongwen Wang Chongwen Wang Shengqi Wang Bing Gu Bing Gu |
| author_facet | Luoluo Shi Luoluo Shi Luoluo Shi Ling Xu Ling Xu Rui Xiao Zihui Zhou Chongwen Wang Chongwen Wang Chongwen Wang Shengqi Wang Bing Gu Bing Gu |
| author_sort | Luoluo Shi |
| collection | DOAJ |
| description | Escherichia coli O157:H7 is regarded as one of the most harmful pathogenic microorganisms related to foodborne diseases. This paper proposes a rapid-detection biosensor for the sensitive and quantitative analysis of E. coli O157:H7 in biological samples by surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFIA). A novel gold-shell silica-core (SiO2/Au) nanosphere (NP) with monodispersity, good stability, and excellent SERS activity was utilized to prepare high-performance tags for the SERS-based LFIA system. The SiO2/Au SERS tags, which were modified with two layers of Raman reporter molecules and monoclonal antibodies, effectively bind with E. coli O157:H7 and form sandwich immune complexes on the test lines. E. coli O157:H7 was quantitatively detected easily by detecting the Raman intensity of the test lines. Under optimal conditions, the limit of detection (LOD) of the SiO2/Au-based SERS-LIFA strips for the target bacteria was 50 cells/mL in PBS solution, indicating these strips are 2,000 times more sensitive than colloidal Au-based LFIA strips. Moreover, the proposed assay demonstrated high applicability in E. coli O157:H7 detection in biological samples, including tap water, milk, human urine, lettuce extract and beef, with a low LOD of 100 cells/mL. Results indicate that the proposed SERS-based LFIA strip is applicable for the sensitive and quantitative determination of E. coli O157:H7. |
| first_indexed | 2024-12-12T09:26:21Z |
| format | Article |
| id | doaj.art-207e97355f054b1fa93460d8ed248528 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-12T09:26:21Z |
| publishDate | 2020-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-207e97355f054b1fa93460d8ed2485282022-12-22T00:29:01ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-11-011110.3389/fmicb.2020.596005596005Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow ImmunoassayLuoluo Shi0Luoluo Shi1Luoluo Shi2Ling Xu3Ling Xu4Rui Xiao5Zihui Zhou6Chongwen Wang7Chongwen Wang8Chongwen Wang9Shengqi Wang10Bing Gu11Bing Gu12Medical Technology Institute of Xuzhou Medical University, Xuzhou, ChinaBeijing Institute of Radiation Medicine, Beijing, ChinaDepartment of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaMedical Technology Institute of Xuzhou Medical University, Xuzhou, ChinaDepartment of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaBeijing Institute of Radiation Medicine, Beijing, ChinaCollege of Life Sciences, Anhui Agricultural University, Hefei, ChinaMedical Technology Institute of Xuzhou Medical University, Xuzhou, ChinaBeijing Institute of Radiation Medicine, Beijing, ChinaCollege of Life Sciences, Anhui Agricultural University, Hefei, ChinaBeijing Institute of Radiation Medicine, Beijing, ChinaMedical Technology Institute of Xuzhou Medical University, Xuzhou, ChinaDepartment of Laboratory Medicine, Affiliated Hospital of Xuzhou Medical University, Xuzhou, ChinaEscherichia coli O157:H7 is regarded as one of the most harmful pathogenic microorganisms related to foodborne diseases. This paper proposes a rapid-detection biosensor for the sensitive and quantitative analysis of E. coli O157:H7 in biological samples by surface-enhanced Raman scattering (SERS)-based lateral flow immunoassay (LFIA). A novel gold-shell silica-core (SiO2/Au) nanosphere (NP) with monodispersity, good stability, and excellent SERS activity was utilized to prepare high-performance tags for the SERS-based LFIA system. The SiO2/Au SERS tags, which were modified with two layers of Raman reporter molecules and monoclonal antibodies, effectively bind with E. coli O157:H7 and form sandwich immune complexes on the test lines. E. coli O157:H7 was quantitatively detected easily by detecting the Raman intensity of the test lines. Under optimal conditions, the limit of detection (LOD) of the SiO2/Au-based SERS-LIFA strips for the target bacteria was 50 cells/mL in PBS solution, indicating these strips are 2,000 times more sensitive than colloidal Au-based LFIA strips. Moreover, the proposed assay demonstrated high applicability in E. coli O157:H7 detection in biological samples, including tap water, milk, human urine, lettuce extract and beef, with a low LOD of 100 cells/mL. Results indicate that the proposed SERS-based LFIA strip is applicable for the sensitive and quantitative determination of E. coli O157:H7.https://www.frontiersin.org/articles/10.3389/fmicb.2020.596005/fullSERSlateral flow immunoassayE. coli O157:H7SiO2/Auquantitative detection |
| spellingShingle | Luoluo Shi Luoluo Shi Luoluo Shi Ling Xu Ling Xu Rui Xiao Zihui Zhou Chongwen Wang Chongwen Wang Chongwen Wang Shengqi Wang Bing Gu Bing Gu Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay Frontiers in Microbiology SERS lateral flow immunoassay E. coli O157:H7 SiO2/Au quantitative detection |
| title | Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay |
| title_full | Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay |
| title_fullStr | Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay |
| title_full_unstemmed | Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay |
| title_short | Rapid, Quantitative, High-Sensitive Detection of Escherichia coli O157:H7 by Gold-Shell Silica-Core Nanospheres-Based Surface-Enhanced Raman Scattering Lateral Flow Immunoassay |
| title_sort | rapid quantitative high sensitive detection of escherichia coli o157 h7 by gold shell silica core nanospheres based surface enhanced raman scattering lateral flow immunoassay |
| topic | SERS lateral flow immunoassay E. coli O157:H7 SiO2/Au quantitative detection |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2020.596005/full |
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