Diagnosis of Canine Visceral Leishmaniasis by ELISA Using K39sub Recombinant Antigen
Background: Surveillance of the canine reservoir is highly important to help control of visceral leishmaniasis in human. It is therefore imperative to improve and develop new tools reliable, easy to use, and cheap for the diagnosis of canine leishmani¬asis. K39 sub recombinant antigen of Leishmania...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2007-06-01
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| Series: | Iranian Journal of Public Health |
| Subjects: | |
| Online Access: | https://ijph.tums.ac.ir/index.php/ijph/article/view/2111 |
| _version_ | 1819239544639193088 |
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| author | M Taran M Mohebali MH Modaresi S Mamishi M Mahmoudi M Mojarad |
| author_facet | M Taran M Mohebali MH Modaresi S Mamishi M Mahmoudi M Mojarad |
| author_sort | M Taran |
| collection | DOAJ |
| description | Background: Surveillance of the canine reservoir is highly important to help control of visceral leishmaniasis in human. It is therefore imperative to improve and develop new tools reliable, easy to use, and cheap for the diagnosis of canine leishmani¬asis. K39 sub recombinant antigen of Leishmania infantum was expressed in prokaryotic system and evaluated for sero-diagnosis of canine visceral leishmaniasis (CVL).
Methods: The gene fragment encoding a single 39-amino acid subunit of the kinesin-related protein k39 (k39sub) was ampli¬fied from DNA of Iranian strain of L. infantum (MCAN/IR/96/LON49) and cloned into a pMAL-p2 expres¬sion vector in frame with maltose-binding protein (MBP) fusion. The antigenic properties of L. infantum recombinant K39 sub¬unit (39 amino acids) have been tested for the serological diagnosis of CVL by ELISA. K39sub ELISA for CVL was com¬pared with a standard direct agglutination test (DAT) on 55 clinically infected dogs and 71 healthy controls from en¬demic areas of Ardabil and East Azerbaijan provinces, north-west of Iran.
Results: A sensitivity of 72.7% and specificity of 87.3% were found at a 1:320 cut off titer when DAT confirmed cases were compared with healthy control. A good concordance was found between k39sub ELISA and DAT (k= 81.0).
Conclusion: Given the antigenic properties shown by the k39sub, we think this protein carry immunodominant epitopes and are valuable for the sero- diagnosis of L. infantum infection in dogs. |
| first_indexed | 2024-12-23T13:53:49Z |
| format | Article |
| id | doaj.art-207eb3120f8648828b4244c70d0e982c |
| institution | Directory Open Access Journal |
| issn | 2251-6085 2251-6093 |
| language | English |
| last_indexed | 2024-12-23T13:53:49Z |
| publishDate | 2007-06-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Public Health |
| spelling | doaj.art-207eb3120f8648828b4244c70d0e982c2022-12-21T17:44:32ZengTehran University of Medical SciencesIranian Journal of Public Health2251-60852251-60932007-06-01362Diagnosis of Canine Visceral Leishmaniasis by ELISA Using K39sub Recombinant Antigen M Taran0 M Mohebali1 MH Modaresi2 S Mamishi3 M Mahmoudi4 M Mojarad5 Background: Surveillance of the canine reservoir is highly important to help control of visceral leishmaniasis in human. It is therefore imperative to improve and develop new tools reliable, easy to use, and cheap for the diagnosis of canine leishmani¬asis. K39 sub recombinant antigen of Leishmania infantum was expressed in prokaryotic system and evaluated for sero-diagnosis of canine visceral leishmaniasis (CVL). Methods: The gene fragment encoding a single 39-amino acid subunit of the kinesin-related protein k39 (k39sub) was ampli¬fied from DNA of Iranian strain of L. infantum (MCAN/IR/96/LON49) and cloned into a pMAL-p2 expres¬sion vector in frame with maltose-binding protein (MBP) fusion. The antigenic properties of L. infantum recombinant K39 sub¬unit (39 amino acids) have been tested for the serological diagnosis of CVL by ELISA. K39sub ELISA for CVL was com¬pared with a standard direct agglutination test (DAT) on 55 clinically infected dogs and 71 healthy controls from en¬demic areas of Ardabil and East Azerbaijan provinces, north-west of Iran. Results: A sensitivity of 72.7% and specificity of 87.3% were found at a 1:320 cut off titer when DAT confirmed cases were compared with healthy control. A good concordance was found between k39sub ELISA and DAT (k= 81.0). Conclusion: Given the antigenic properties shown by the k39sub, we think this protein carry immunodominant epitopes and are valuable for the sero- diagnosis of L. infantum infection in dogs.https://ijph.tums.ac.ir/index.php/ijph/article/view/2111K39antigenLeishmania infantumDogs |
| spellingShingle | M Taran M Mohebali MH Modaresi S Mamishi M Mahmoudi M Mojarad Diagnosis of Canine Visceral Leishmaniasis by ELISA Using K39sub Recombinant Antigen Iranian Journal of Public Health K39antigen Leishmania infantum Dogs |
| title | Diagnosis of Canine Visceral Leishmaniasis by ELISA Using K39sub Recombinant Antigen |
| title_full | Diagnosis of Canine Visceral Leishmaniasis by ELISA Using K39sub Recombinant Antigen |
| title_fullStr | Diagnosis of Canine Visceral Leishmaniasis by ELISA Using K39sub Recombinant Antigen |
| title_full_unstemmed | Diagnosis of Canine Visceral Leishmaniasis by ELISA Using K39sub Recombinant Antigen |
| title_short | Diagnosis of Canine Visceral Leishmaniasis by ELISA Using K39sub Recombinant Antigen |
| title_sort | diagnosis of canine visceral leishmaniasis by elisa using k39sub recombinant antigen |
| topic | K39antigen Leishmania infantum Dogs |
| url | https://ijph.tums.ac.ir/index.php/ijph/article/view/2111 |
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