Protocol for the isolation and culture of microglia, astrocytes, and neurons from the same mouse brain

Protocol for the isolation and culture of microglia, astrocytes, and neurons from the same mouse brain

Show other versions (1)

Bibliographic Details
Main Authors:	Elvira P. Leites, Vanessa A. Morais
Format:	Article
Language:	English
Published:	Elsevier 2024-03-01
Series:	STAR Protocols
Online Access:	http://www.sciencedirect.com/science/article/pii/S2666166724000340

Similar Items

Protocol for the isolation and culture of microglia, astrocytes, and neurons from the same mouse brain
by: Elvira P. Leites, et al.
Published: (2024-03-01)

Protocol for Tri-culture of hiPSC-Derived Neurons, Astrocytes, and Microglia
by: Sean K. Ryan, et al.
Published: (2020-12-01)

Isolation and culture of pure adult mouse microglia and astrocytes for in vitro characterization and analyses
by: Mark T. Milner, et al.
Published: (2022-06-01)

Sequential Isolation of Microglia and Astrocytes from Young and Aged Adult Mouse Brains for Downstream Transcriptomic Analysis
by: Ruchelle G. Buenaventura, et al.
Published: (2022-09-01)

NLRP3 Inflammasome Is Expressed and Functional in Mouse Brain Microglia but Not in Astrocytes.
by: Audrey Gustin, et al.
Published: (2015-01-01)

Apoptosis and necroptosis of mouse hippocampal and parenchymal astrocytes, microglia and neurons caused by Angiostrongylus cantonensis infection
by: Zhang Mengying, et al.
Published: (2017-12-01)

Differential usage of DNA modifications in neurons, astrocytes, and microglia
by: Kyla B. Tooley, et al.
Published: (2023-11-01)

Microglia Don’t Treat All Neurons the Same: The Importance of Neuronal Subtype in Microglia-Neuron Interactions in the Developing Hypothalamus
by: Zuri Ngozi, et al.
Published: (2022-04-01)

Co-cultures of Glioma Stem Cells and Primary Neurons, Astrocytes, Microglia, and Endothelial Cells for Investigation of Intercellular Communication in the Brain
by: Zhiyun Wei, et al.
Published: (2019-04-01)

A Neuron, Microglia, and Astrocyte Triple Co-culture Model to Study Alzheimer’s Disease
by: Celia Luchena, et al.
Published: (2022-04-01)

The neuron-astrocyte-microglia triad in normal brain ageing and in a model of neuroinflammation in the rat hippocampus.
by: Francesca Cerbai, et al.
Published: (2012-01-01)

A primary neural cell culture model to study neuron, astrocyte, and microglia interactions in neuroinflammation
by: Noah Goshi, et al.
Published: (2020-05-01)

Improved modeling of human AD with an automated culturing platform for iPSC neurons, astrocytes and microglia
by: Reina Bassil, et al.
Published: (2021-09-01)

Effects of Cerebrospinal Fluids from Alzheimer and Non-Alzheimer Patients on Neurons–Astrocytes–Microglia Co-Culture
by: Matilda Iemmolo, et al.
Published: (2024-02-01)

Isolation of endothelial cells, pericytes and astrocytes from mouse brain.
by: Florian Bernard-Patrzynski, et al.
Published: (2019-01-01)

LIPOTYPE OF NEURONS, ASTROCYTES, OLIGODENDROCYTES, MICROGLIA AND EPENDYMAL CELLS IN THE RAT CNS
by: Jonatan Martínez-Gardeazabal, et al.
Published: (2023-10-01)

LPS-Induced Systemic Inflammation Affects the Dynamic Interactions of Astrocytes and Microglia with the Vasculature of the Mouse Brain Cortex
by: Evangelia Xingi, et al.
Published: (2023-05-01)

BIN1 protein isoforms are differentially expressed in astrocytes, neurons, and microglia: neuronal and astrocyte BIN1 are implicated in tau pathology
by: Mariko Taga, et al.
Published: (2020-07-01)

BrainPhys Neuronal Media Support Physiological Function of Mitochondria in Mouse Primary Neuronal Cultures
by: Andreia Faria-Pereira, et al.
Published: (2022-06-01)

Microglia Negatively Regulate the Proliferation and Neuronal Differentiation of Neural Stem/Progenitor Cells Isolated from Poststroke Mouse Brains
by: Yoshinobu Hirano, et al.
Published: (2023-08-01)

Evidence that erythropoietin modulates neuroinflammation through differential action on neurons, astrocytes, and microglia
by: Wesley S Bond, et al.
Published: (2014-10-01)

The Emerging Role of the Interplay Among Astrocytes, Microglia, and Neurons in the Hippocampus in Health and Disease
by: Daniele Lana, et al.
Published: (2021-04-01)

Tropism of Newcastle disease virus strains for chicken neurons, astrocytes, oligodendrocytes, and microglia
by: Salman L. Butt, et al.
Published: (2019-09-01)

Liposomal clodronate selectively eliminates microglia from primary astrocyte cultures
by: Kumamaru Hiromi, et al.
Published: (2012-05-01)

Efficient isolation of live microglia with preserved phenotypes from adult mouse brain
by: Nikodemova Maria, et al.
Published: (2012-06-01)

MATCHING ASTROCYTIC AND NEURONAL METABOLISM FOR BRAIN FUNCTION
by: Juan P. Bolanos
Published: (2023-10-01)

Isolation and culturing of primary mouse astrocytes for the analysis of focal adhesion dynamics
by: Baran E. Güler, et al.
Published: (2021-12-01)

Protocol for the isolation and culture of mouse dorsal root ganglion neurons for imaging applications
by: Patrick R. Smith, et al.
Published: (2023-12-01)

Interaction of Microglia and Astrocytes in the Neurovascular Unit
by: Li-rong Liu, et al.
Published: (2020-07-01)

Microglia and astrocyte dysfunction in parkinson's disease
by: Tae-In Kam, et al.
Published: (2020-10-01)

Astrocyte–Oligodendrocyte–Microglia Crosstalk in Astrocytopathies
by: Dieuwke Maria de Waard, et al.
Published: (2020-11-01)

Crosstalk Between Astrocytes and Microglia: An Overview
by: Agata Matejuk, et al.
Published: (2020-07-01)

Isolation of Microglia from Mouse or Human Tissue
by: Evan A. Bordt, et al.
Published: (2020-06-01)

Microglia replacement by microglia transplantation (Mr MT) in the adult mouse brain
by: Zhen Xu, et al.
Published: (2021-09-01)

Bacteria reduce flagellin synthesis to evade microglia-astrocyte-driven immunity in the brain
by: Hao Sun, et al.
Published: (2022-07-01)

Buprenorphine alters microglia and astrocytes acutely following diffuse traumatic brain injury
by: Jane Ryu, et al.
Published: (2021-04-01)

Age-associated changes in microglia and astrocytes ameliorate blood-brain barrier dysfunction
by: Jie Pan, et al.
Published: (2021-12-01)

Pathogenic LRRK2 control of primary cilia and Hedgehog signaling in neurons and astrocytes of mouse brain
by: Shahzad S Khan, et al.
Published: (2021-10-01)

Proposed practical protocol for flow cytometry analysis of microglia from the healthy adult mouse brain: Systematic review and isolation methods’ evaluation
by: Sanja Srakočić, et al.
Published: (2022-10-01)

Neuronal and Astrocytic Extracellular Vesicle Biomarkers in Blood Reflect Brain Pathology in Mouse Models of Alzheimer’s Disease
by: Francheska Delgado-Peraza, et al.
Published: (2021-04-01)