Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infection
Abstract Background Puccinia arachidis fungus causes rust disease in the peanut plants (Arachis hypogaea L.), which leads to high yield loss. Metabolomic profiling of Arachis hypogaea was performed to identify the pathogen-induced production of metabolites involved in the defense mechanism of peanut...
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| Format: | Article |
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BMC
2023-10-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-023-09725-3 |
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| author | Visha Rathod Khyati Rathod Rukam S. Tomar Ritisha Tatamiya Rasmieh Hamid Feba Jacob Nasreen Shakil Munshi |
| author_facet | Visha Rathod Khyati Rathod Rukam S. Tomar Ritisha Tatamiya Rasmieh Hamid Feba Jacob Nasreen Shakil Munshi |
| author_sort | Visha Rathod |
| collection | DOAJ |
| description | Abstract Background Puccinia arachidis fungus causes rust disease in the peanut plants (Arachis hypogaea L.), which leads to high yield loss. Metabolomic profiling of Arachis hypogaea was performed to identify the pathogen-induced production of metabolites involved in the defense mechanism of peanut plants. In this study, two peanut genotypes, one susceptible (JL-24) and one resistant (GPBD-4) were inoculated with Puccinia arachidis fungal pathogen. The metabolic response was assessed at the control stage (0 day without inoculation), 2 DAI (Day after inoculation), 4 DAI and 6 DAI by Gas Chromatography-Mass Spectrometry (GC-MS). Results About 61 metabolites were identified by NIST library, comprising sugars, phenols, fatty acids, carboxylic acids and sugar alcohols. Sugars and fatty acids were predominant in leaf extracts compared to other metabolites. Concentration of different metabolites such as salicylic acid, mannitol, flavonoid, 9,12-octadecadienoic acid, linolenic acid and glucopyranoside were higher in resistant genotype than in susceptible genotype during infection. Systemic acquired resistance (SAR) and hypersensitive reaction (HR) components such as oxalic acid was elevated in resistant genotype during pathogen infection. Partial least square-discriminant analysis (PLS-DA) was applied to GC-MS data for revealing metabolites profile between resistant and susceptible genotype during infection. Conclusion The phenol content and oxidative enzyme activity i.e. catalase, peroxidase and polyphenol oxidase were found to be very high at 4 DAI in resistant genotype (p-value < 0.01). This metabolic approach provides information about bioactive plant metabolites and their application in crop protection and marker-assisted plant breeding. |
| first_indexed | 2024-03-10T22:15:03Z |
| format | Article |
| id | doaj.art-2081cc4574a64fd6bc35a969f9ac2f7a |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-03-10T22:15:03Z |
| publishDate | 2023-10-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-2081cc4574a64fd6bc35a969f9ac2f7a2023-11-19T12:28:06ZengBMCBMC Genomics1471-21642023-10-0124111510.1186/s12864-023-09725-3Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infectionVisha Rathod0Khyati Rathod1Rukam S. Tomar2Ritisha Tatamiya3Rasmieh Hamid4Feba Jacob5Nasreen Shakil Munshi6Institute of Science, Nirma UniversityDepartment of Biotechnology and Biochemistry, Junagadh Agricultural UniversityDepartment of Biotechnology and Biochemistry, Junagadh Agricultural UniversityDepartment of Bioscience, Saurashtra UniversityDepartment of Plant Breeding, Cotton Research Institute of Iran (CRII), Agricultural Research, Education and Extension Organization (AREEO)Centre for Plant Biotechnology and Molecular Biology, Kerala Agricultural UniversityInstitute of Science, Nirma UniversityAbstract Background Puccinia arachidis fungus causes rust disease in the peanut plants (Arachis hypogaea L.), which leads to high yield loss. Metabolomic profiling of Arachis hypogaea was performed to identify the pathogen-induced production of metabolites involved in the defense mechanism of peanut plants. In this study, two peanut genotypes, one susceptible (JL-24) and one resistant (GPBD-4) were inoculated with Puccinia arachidis fungal pathogen. The metabolic response was assessed at the control stage (0 day without inoculation), 2 DAI (Day after inoculation), 4 DAI and 6 DAI by Gas Chromatography-Mass Spectrometry (GC-MS). Results About 61 metabolites were identified by NIST library, comprising sugars, phenols, fatty acids, carboxylic acids and sugar alcohols. Sugars and fatty acids were predominant in leaf extracts compared to other metabolites. Concentration of different metabolites such as salicylic acid, mannitol, flavonoid, 9,12-octadecadienoic acid, linolenic acid and glucopyranoside were higher in resistant genotype than in susceptible genotype during infection. Systemic acquired resistance (SAR) and hypersensitive reaction (HR) components such as oxalic acid was elevated in resistant genotype during pathogen infection. Partial least square-discriminant analysis (PLS-DA) was applied to GC-MS data for revealing metabolites profile between resistant and susceptible genotype during infection. Conclusion The phenol content and oxidative enzyme activity i.e. catalase, peroxidase and polyphenol oxidase were found to be very high at 4 DAI in resistant genotype (p-value < 0.01). This metabolic approach provides information about bioactive plant metabolites and their application in crop protection and marker-assisted plant breeding.https://doi.org/10.1186/s12864-023-09725-3GC-MSArachis hypogaeaPuccinia ArachidisMetabolomics, pathogen InfectionPhenolics |
| spellingShingle | Visha Rathod Khyati Rathod Rukam S. Tomar Ritisha Tatamiya Rasmieh Hamid Feba Jacob Nasreen Shakil Munshi Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infection BMC Genomics GC-MS Arachis hypogaea Puccinia Arachidis Metabolomics, pathogen Infection Phenolics |
| title | Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infection |
| title_full | Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infection |
| title_fullStr | Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infection |
| title_full_unstemmed | Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infection |
| title_short | Metabolic profiles of peanut (Arachis hypogaea L.) in response to Puccinia arachidis fungal infection |
| title_sort | metabolic profiles of peanut arachis hypogaea l in response to puccinia arachidis fungal infection |
| topic | GC-MS Arachis hypogaea Puccinia Arachidis Metabolomics, pathogen Infection Phenolics |
| url | https://doi.org/10.1186/s12864-023-09725-3 |
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