Matrix metalloproteinase-1 expression in oral submucous fibrosis: An immunohistochemical study
<b>Context:</b> Oral submucous fibrosis (OSF) is a form of pathological fibrosis affecting the oral mucosa. There is compelling evidence to implicate the habitual chewing of areca nut with the development of OSF. Because collagens are the major structural components of connective tissue...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2010-01-01
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| Series: | Indian Journal of Dental Research |
| Subjects: | |
| Online Access: | http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=3;spage=320;epage=325;aulast=Mishra |
| _version_ | 1818021026445918208 |
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| author | Mishra Gauri Ranganathan K |
| author_facet | Mishra Gauri Ranganathan K |
| author_sort | Mishra Gauri |
| collection | DOAJ |
| description | <b>Context:</b> Oral submucous fibrosis (OSF) is a form of pathological fibrosis affecting the oral mucosa. There is compelling evidence to implicate the habitual chewing of areca nut with the development of OSF. Because collagens are the major structural components of connective tissues, including oral submucosa, the composition of collagen within each tissue needs to be precisely regulated to maintain tissue integrity. Arecoline stimulates fibroblasts to increase the production of collagen by 150%. <b>Aim:</b> As the role of collagenase is implicated in cleaving the collagen under physical conditions, this study was carried out to evaluate the role of collagenase-1 (matrix metalloproteinase [MMP]-1) in a pathologic condition like OSF. <b>Settings and Design:</b> A total of 40 patients were included in the study, comprising of 30 OSF as Group 1 and 10 normal buccal mucosa tissue as Group 2. <b>Materials and Methods:</b> Both the groups were stained for MMP-1 by the immunohistochemical method using the streptavidin HRP-biotin labeling technique. MMP-1 expression intensity in the epithelium and connective tissue was decreased in Group 1 when compared to Group 2. <b>Statistical Analysis Used:</b> Chi-square test of association was used to determine the difference in the expression of MMP-1 between OSF and normal buccal mucosa and among different histological gradings of OSF. <b>Results:</b> The results were statistically significant. However, there was no statistically significant difference between the expression of MMP-1 among different histological grades of OSF in Group 1. |
| first_indexed | 2024-04-14T08:13:30Z |
| format | Article |
| id | doaj.art-2082405352974039b67d4be4bf69e71b |
| institution | Directory Open Access Journal |
| issn | 0970-9290 1998-3603 |
| language | English |
| last_indexed | 2024-04-14T08:13:30Z |
| publishDate | 2010-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Indian Journal of Dental Research |
| spelling | doaj.art-2082405352974039b67d4be4bf69e71b2022-12-22T02:04:28ZengWolters Kluwer Medknow PublicationsIndian Journal of Dental Research0970-92901998-36032010-01-01213320325Matrix metalloproteinase-1 expression in oral submucous fibrosis: An immunohistochemical studyMishra GauriRanganathan K<b>Context:</b> Oral submucous fibrosis (OSF) is a form of pathological fibrosis affecting the oral mucosa. There is compelling evidence to implicate the habitual chewing of areca nut with the development of OSF. Because collagens are the major structural components of connective tissues, including oral submucosa, the composition of collagen within each tissue needs to be precisely regulated to maintain tissue integrity. Arecoline stimulates fibroblasts to increase the production of collagen by 150%. <b>Aim:</b> As the role of collagenase is implicated in cleaving the collagen under physical conditions, this study was carried out to evaluate the role of collagenase-1 (matrix metalloproteinase [MMP]-1) in a pathologic condition like OSF. <b>Settings and Design:</b> A total of 40 patients were included in the study, comprising of 30 OSF as Group 1 and 10 normal buccal mucosa tissue as Group 2. <b>Materials and Methods:</b> Both the groups were stained for MMP-1 by the immunohistochemical method using the streptavidin HRP-biotin labeling technique. MMP-1 expression intensity in the epithelium and connective tissue was decreased in Group 1 when compared to Group 2. <b>Statistical Analysis Used:</b> Chi-square test of association was used to determine the difference in the expression of MMP-1 between OSF and normal buccal mucosa and among different histological gradings of OSF. <b>Results:</b> The results were statistically significant. However, there was no statistically significant difference between the expression of MMP-1 among different histological grades of OSF in Group 1.http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=3;spage=320;epage=325;aulast=MishraArecanutmatrix metalloproteinaseoral submucous fibrosis |
| spellingShingle | Mishra Gauri Ranganathan K Matrix metalloproteinase-1 expression in oral submucous fibrosis: An immunohistochemical study Indian Journal of Dental Research Arecanut matrix metalloproteinase oral submucous fibrosis |
| title | Matrix metalloproteinase-1 expression in oral submucous fibrosis: An immunohistochemical study |
| title_full | Matrix metalloproteinase-1 expression in oral submucous fibrosis: An immunohistochemical study |
| title_fullStr | Matrix metalloproteinase-1 expression in oral submucous fibrosis: An immunohistochemical study |
| title_full_unstemmed | Matrix metalloproteinase-1 expression in oral submucous fibrosis: An immunohistochemical study |
| title_short | Matrix metalloproteinase-1 expression in oral submucous fibrosis: An immunohistochemical study |
| title_sort | matrix metalloproteinase 1 expression in oral submucous fibrosis an immunohistochemical study |
| topic | Arecanut matrix metalloproteinase oral submucous fibrosis |
| url | http://www.ijdr.in/article.asp?issn=0970-9290;year=2010;volume=21;issue=3;spage=320;epage=325;aulast=Mishra |
| work_keys_str_mv | AT mishragauri matrixmetalloproteinase1expressioninoralsubmucousfibrosisanimmunohistochemicalstudy AT ranganathank matrixmetalloproteinase1expressioninoralsubmucousfibrosisanimmunohistochemicalstudy |