Expression profile of sox5 and sox6 in sertoli and spermatogonial cells in growing mice testis
SRY box genes are peculiar to animal kingdom. They are involved in many processes particularly sex determination and testes development in male embryo. Although Sox family genes have been identified in various cells, their expression pattern and role is not entirely recognized in Sertoli cells. In t...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Kafkas University, Faculty of Veterinary Medicine
2019-10-01
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| Series: | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| Subjects: | |
| Online Access: | https://vetdergikafkas.org/pdf.php?id=2653 |
| _version_ | 1827922158955790336 |
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| author | Zia Ur REHMAN Qing LIU Dinesh BHATTARAI Aixin LIANG Farman ULLAH Rahim DAD Sher Bahadur KHAN Hira Sajjad TALPUR Sher Ali KHAN Liguo YANG |
| author_facet | Zia Ur REHMAN Qing LIU Dinesh BHATTARAI Aixin LIANG Farman ULLAH Rahim DAD Sher Bahadur KHAN Hira Sajjad TALPUR Sher Ali KHAN Liguo YANG |
| author_sort | Zia Ur REHMAN |
| collection | DOAJ |
| description | SRY box genes are peculiar to animal kingdom. They are involved in many processes particularly sex determination and testes development
in male embryo. Although Sox family genes have been identified in various cells, their expression pattern and role is not entirely recognized
in Sertoli cells. In this research, we focused on the expression of SoxD group Sox5 and Sox6 genes in Sertoli cells of mice during pre- and postpubertal
testicular development ranging from one-week-old to eight-week-old mice. The expression was studied by immunohistochemistry on
whole testes, and qPCR to determine the mRNA level of all age groups, while immunocyto-chemistry was performed for localization in specific
age groups. qPCR results of Sertoli cells from first week to eight week showed different levels of expression. The mRNA level of Sox 5, during prepubertal
age, was significantly high (P<0.001), but as the age progressed, the expression became low. Conversely, Sox6 was initially expressed
faintly, but at the pubertal age, the expression rose significantly (P<0.001). Furthermore, the expression signals of both genes on spermatogonial
cells were also found strong. The study shows that Sox5 and Sox6 are expressed during postnatal and pubertal periods and may play a vital role
in the maturation of spermatozoa. In addition, they overlap to regulate multiple functions like spermatogenesis and steroidogenesis in testes. |
| first_indexed | 2024-03-13T04:38:01Z |
| format | Article |
| id | doaj.art-20836c38c1f2407695a492af5b8285a0 |
| institution | Directory Open Access Journal |
| issn | 1309-2251 |
| language | English |
| last_indexed | 2024-03-13T04:38:01Z |
| publishDate | 2019-10-01 |
| publisher | Kafkas University, Faculty of Veterinary Medicine |
| record_format | Article |
| series | Kafkas Universitesi Veteriner Fakültesi Dergisi |
| spelling | doaj.art-20836c38c1f2407695a492af5b8285a02023-06-19T06:33:12ZengKafkas University, Faculty of Veterinary MedicineKafkas Universitesi Veteriner Fakültesi Dergisi1309-22512019-10-0126110310910.9775/kvfd.2019.224132653Expression profile of sox5 and sox6 in sertoli and spermatogonial cells in growing mice testisZia Ur REHMAN0Qing LIU1Dinesh BHATTARAI2Aixin LIANG3Farman ULLAH4Rahim DAD5Sher Bahadur KHAN6Hira Sajjad TALPUR7Sher Ali KHANLiguo YANG8Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR CHINAKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR CHINAKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR CHINAKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR CHINAFaculty of Animal Husbandry and Veterinary Science, Lasbela University of Agriculture, Water and Marine Sciences (LUAWMS), 90150, PAKISTANFaculty of Animal Husbandry and Veterinary Science, Sindh Agriculture University, Tandojam, 70060, PAKISTANDepartment of Animal Health, Faculty of Animal Husbandry and Veterinary Science, The University of Agriculture, Peshawar, 2500, PAKISTANFaculty of Animal Husbandry and Veterinary Science, Sindh Agriculture University, Tandojam, 70060, PAKISTANKey Laboratory of Agricultural Animal Genetics, Breeding and Reproduction, Education Ministry of China, College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 430070, PR CHINASRY box genes are peculiar to animal kingdom. They are involved in many processes particularly sex determination and testes development in male embryo. Although Sox family genes have been identified in various cells, their expression pattern and role is not entirely recognized in Sertoli cells. In this research, we focused on the expression of SoxD group Sox5 and Sox6 genes in Sertoli cells of mice during pre- and postpubertal testicular development ranging from one-week-old to eight-week-old mice. The expression was studied by immunohistochemistry on whole testes, and qPCR to determine the mRNA level of all age groups, while immunocyto-chemistry was performed for localization in specific age groups. qPCR results of Sertoli cells from first week to eight week showed different levels of expression. The mRNA level of Sox 5, during prepubertal age, was significantly high (P<0.001), but as the age progressed, the expression became low. Conversely, Sox6 was initially expressed faintly, but at the pubertal age, the expression rose significantly (P<0.001). Furthermore, the expression signals of both genes on spermatogonial cells were also found strong. The study shows that Sox5 and Sox6 are expressed during postnatal and pubertal periods and may play a vital role in the maturation of spermatozoa. In addition, they overlap to regulate multiple functions like spermatogenesis and steroidogenesis in testes.https://vetdergikafkas.org/pdf.php?id=2653sertoli cellsspermatogenesissox5sox6testis |
| spellingShingle | Zia Ur REHMAN Qing LIU Dinesh BHATTARAI Aixin LIANG Farman ULLAH Rahim DAD Sher Bahadur KHAN Hira Sajjad TALPUR Sher Ali KHAN Liguo YANG Expression profile of sox5 and sox6 in sertoli and spermatogonial cells in growing mice testis Kafkas Universitesi Veteriner Fakültesi Dergisi sertoli cells spermatogenesis sox5 sox6 testis |
| title | Expression profile of sox5 and sox6 in sertoli and spermatogonial cells in growing mice testis |
| title_full | Expression profile of sox5 and sox6 in sertoli and spermatogonial cells in growing mice testis |
| title_fullStr | Expression profile of sox5 and sox6 in sertoli and spermatogonial cells in growing mice testis |
| title_full_unstemmed | Expression profile of sox5 and sox6 in sertoli and spermatogonial cells in growing mice testis |
| title_short | Expression profile of sox5 and sox6 in sertoli and spermatogonial cells in growing mice testis |
| title_sort | expression profile of sox5 and sox6 in sertoli and spermatogonial cells in growing mice testis |
| topic | sertoli cells spermatogenesis sox5 sox6 testis |
| url | https://vetdergikafkas.org/pdf.php?id=2653 |
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