The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea
Pathogen stress often significantly decreases cucumber production. However, knowledge regarding the molecular mechanism and signals of cucumber disease resistance is far from complete. Here, we report two translationally controlled tumor protein genes, CsTCTP1 and CsTCTP2, that are both negative mod...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00544/full |
| _version_ | 1818906407677722624 |
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| author | Xiangnan Meng Xiangnan Meng Yang Yu Junyue Zhao Na Cui Tiefeng Song Yun Yang Haiyan Fan Haiyan Fan |
| author_facet | Xiangnan Meng Xiangnan Meng Yang Yu Junyue Zhao Na Cui Tiefeng Song Yun Yang Haiyan Fan Haiyan Fan |
| author_sort | Xiangnan Meng |
| collection | DOAJ |
| description | Pathogen stress often significantly decreases cucumber production. However, knowledge regarding the molecular mechanism and signals of cucumber disease resistance is far from complete. Here, we report two translationally controlled tumor protein genes, CsTCTP1 and CsTCTP2, that are both negative modulators in the Cucumis sativus defense response to Sphaerotheca fuliginea. Subcellular localization analysis showed that CsTCTP1 and CsTCTP2 were both localized in the cytoplasm. Expression analysis indicated that the transcript levels of CsTCTP1 and CsTCTP2 were linked to the degree of cucumber resistance to S. fuliginea. Transient overexpression of either CsTCTP1 or CsTCTP2 in cucumber cotyledons impaired resistance to S. fuliginea, whereas silencing of either CsTCTP1 or CsTCTP2 enhanced cucumber resistance to S. fuliginea. The relationship of several defense-related genes and ABA and target of rapamycin (TOR) signaling pathway-related genes to the overexpressing and silencing of CsTCTP1/CsTCTP2 in non-infested cucumber plants was investigated. The results indicated that CsTCTP1 participates in the defense response to S. fuliginea by regulating the expression of certain defense-associated genes and/or ABA signaling pathway-associated genes, and CsTCTP2 participates through regulating the expression of TOR signaling pathway-associated genes. Our findings will guide enhancing the resistance of cucumber to powdery mildew. |
| first_indexed | 2024-12-19T21:38:44Z |
| format | Article |
| id | doaj.art-2084ec11e42a4362afa2ac83d9f22821 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-19T21:38:44Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-2084ec11e42a4362afa2ac83d9f228212022-12-21T20:04:43ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-04-01910.3389/fpls.2018.00544354859The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuligineaXiangnan Meng0Xiangnan Meng1Yang Yu2Junyue Zhao3Na Cui4Tiefeng Song5Yun Yang6Haiyan Fan7Haiyan Fan8College of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaCollege of Horticulture, Shenyang Agricultural University, Shenyang, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaVegetable Research Institute, Liaoning Academy of Agricultural Sciences, Shenyang, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaCollege of Bioscience and Biotechnology, Shenyang Agricultural University, Shenyang, ChinaKey Laboratory of Protected Horticulture of Ministry of Education, Shenyang Agricultural University, Shenyang, ChinaPathogen stress often significantly decreases cucumber production. However, knowledge regarding the molecular mechanism and signals of cucumber disease resistance is far from complete. Here, we report two translationally controlled tumor protein genes, CsTCTP1 and CsTCTP2, that are both negative modulators in the Cucumis sativus defense response to Sphaerotheca fuliginea. Subcellular localization analysis showed that CsTCTP1 and CsTCTP2 were both localized in the cytoplasm. Expression analysis indicated that the transcript levels of CsTCTP1 and CsTCTP2 were linked to the degree of cucumber resistance to S. fuliginea. Transient overexpression of either CsTCTP1 or CsTCTP2 in cucumber cotyledons impaired resistance to S. fuliginea, whereas silencing of either CsTCTP1 or CsTCTP2 enhanced cucumber resistance to S. fuliginea. The relationship of several defense-related genes and ABA and target of rapamycin (TOR) signaling pathway-related genes to the overexpressing and silencing of CsTCTP1/CsTCTP2 in non-infested cucumber plants was investigated. The results indicated that CsTCTP1 participates in the defense response to S. fuliginea by regulating the expression of certain defense-associated genes and/or ABA signaling pathway-associated genes, and CsTCTP2 participates through regulating the expression of TOR signaling pathway-associated genes. Our findings will guide enhancing the resistance of cucumber to powdery mildew.http://journal.frontiersin.org/article/10.3389/fpls.2018.00544/fullABA signaling pathwayCucumis sativusSphaerotheca fuligineatranslationally controlled tumor proteintransient transformationTOR signaling pathway |
| spellingShingle | Xiangnan Meng Xiangnan Meng Yang Yu Junyue Zhao Na Cui Tiefeng Song Yun Yang Haiyan Fan Haiyan Fan The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea Frontiers in Plant Science ABA signaling pathway Cucumis sativus Sphaerotheca fuliginea translationally controlled tumor protein transient transformation TOR signaling pathway |
| title | The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea |
| title_full | The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea |
| title_fullStr | The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea |
| title_full_unstemmed | The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea |
| title_short | The Two Translationally Controlled Tumor Protein Genes, CsTCTP1 and CsTCTP2, Are Negative Modulators in the Cucumis sativus Defense Response to Sphaerotheca fuliginea |
| title_sort | two translationally controlled tumor protein genes cstctp1 and cstctp2 are negative modulators in the cucumis sativus defense response to sphaerotheca fuliginea |
| topic | ABA signaling pathway Cucumis sativus Sphaerotheca fuliginea translationally controlled tumor protein transient transformation TOR signaling pathway |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00544/full |
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