Image analysis for the quantitative comparison of stress fibers and focal adhesions.
Actin stress fibers (SFs) detect and transmit forces to the extracellular matrix through focal adhesions (FAs), and molecules in this pathway determine cellular behavior. Here, we designed two different computational tools to quantify actin SFs and the distribution of actin cytoskeletal proteins wit...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0107393 |
| _version_ | 1819100937063497728 |
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| author | Alberto Elosegui-Artola Alvaro Jorge-Peñas Oihana Moreno-Arotzena Amaia Oregi Marta Lasa José Manuel García-Aznar Elena M De Juan-Pardo Rafael Aldabe |
| author_facet | Alberto Elosegui-Artola Alvaro Jorge-Peñas Oihana Moreno-Arotzena Amaia Oregi Marta Lasa José Manuel García-Aznar Elena M De Juan-Pardo Rafael Aldabe |
| author_sort | Alberto Elosegui-Artola |
| collection | DOAJ |
| description | Actin stress fibers (SFs) detect and transmit forces to the extracellular matrix through focal adhesions (FAs), and molecules in this pathway determine cellular behavior. Here, we designed two different computational tools to quantify actin SFs and the distribution of actin cytoskeletal proteins within a normalized cellular morphology. Moreover, a systematic cell response comparison between the control cells and those with impaired actin cytoskeleton polymerization was performed to demonstrate the reliability of the tools. Indeed, a variety of proteins that were present within the string beginning at the focal adhesions (vinculin) up to the actin SFs contraction (non-muscle myosin II (NMMII)) were analyzed. Finally, the software used allows for the quantification of the SFs based on the relative positions of FAs. Therefore, it provides a better insight into the cell mechanics and broadens the knowledge of the nature of SFs. |
| first_indexed | 2024-12-22T01:10:42Z |
| format | Article |
| id | doaj.art-2085c717cb6a40b087b86edeb07f17c5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T01:10:42Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-2085c717cb6a40b087b86edeb07f17c52022-12-21T18:43:58ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10739310.1371/journal.pone.0107393Image analysis for the quantitative comparison of stress fibers and focal adhesions.Alberto Elosegui-ArtolaAlvaro Jorge-PeñasOihana Moreno-ArotzenaAmaia OregiMarta LasaJosé Manuel García-AznarElena M De Juan-PardoRafael AldabeActin stress fibers (SFs) detect and transmit forces to the extracellular matrix through focal adhesions (FAs), and molecules in this pathway determine cellular behavior. Here, we designed two different computational tools to quantify actin SFs and the distribution of actin cytoskeletal proteins within a normalized cellular morphology. Moreover, a systematic cell response comparison between the control cells and those with impaired actin cytoskeleton polymerization was performed to demonstrate the reliability of the tools. Indeed, a variety of proteins that were present within the string beginning at the focal adhesions (vinculin) up to the actin SFs contraction (non-muscle myosin II (NMMII)) were analyzed. Finally, the software used allows for the quantification of the SFs based on the relative positions of FAs. Therefore, it provides a better insight into the cell mechanics and broadens the knowledge of the nature of SFs.https://doi.org/10.1371/journal.pone.0107393 |
| spellingShingle | Alberto Elosegui-Artola Alvaro Jorge-Peñas Oihana Moreno-Arotzena Amaia Oregi Marta Lasa José Manuel García-Aznar Elena M De Juan-Pardo Rafael Aldabe Image analysis for the quantitative comparison of stress fibers and focal adhesions. PLoS ONE |
| title | Image analysis for the quantitative comparison of stress fibers and focal adhesions. |
| title_full | Image analysis for the quantitative comparison of stress fibers and focal adhesions. |
| title_fullStr | Image analysis for the quantitative comparison of stress fibers and focal adhesions. |
| title_full_unstemmed | Image analysis for the quantitative comparison of stress fibers and focal adhesions. |
| title_short | Image analysis for the quantitative comparison of stress fibers and focal adhesions. |
| title_sort | image analysis for the quantitative comparison of stress fibers and focal adhesions |
| url | https://doi.org/10.1371/journal.pone.0107393 |
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