Different expression levels of interleukin-36 in asthma phenotypes
Abstract Interleukin (IL)-36 family is closely associated with inflammation and consists of IL-36α, IL-36β, IL-36γ, and IL-36Ra. The role of IL-36 in the context of asthma and asthmatic phenotypes is not well characterized. We examined the sputum IL-36 levels in patients with different asthma phenot...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | Allergy, Asthma & Clinical Immunology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13223-023-00868-2 |
| _version_ | 1797355698184519680 |
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| author | Jinyan Li Zhengda Wang Hongna Dong Yuqiu Hao Peng Gao Wei Li |
| author_facet | Jinyan Li Zhengda Wang Hongna Dong Yuqiu Hao Peng Gao Wei Li |
| author_sort | Jinyan Li |
| collection | DOAJ |
| description | Abstract Interleukin (IL)-36 family is closely associated with inflammation and consists of IL-36α, IL-36β, IL-36γ, and IL-36Ra. The role of IL-36 in the context of asthma and asthmatic phenotypes is not well characterized. We examined the sputum IL-36 levels in patients with different asthma phenotypes in order to unravel the mechanism of IL-36 in different asthma phenotypes. Our objective was to investigate the induced sputum IL-36α, IL-36β, IL-36γ, and IL-36Ra concentrations in patients with mild asthma, and to analyze the relationship of these markers with lung function and other cytokines in patients with different asthma phenotypes. Induced sputum samples were collected from patients with mild controlled asthma (n = 62, 27 males, age 54.77 ± 15.49) and healthy non-asthmatic controls (n = 16, 10 males, age 54.25 ± 14.60). Inflammatory cell counts in sputum were determined. The concentrations of IL-36 and other cytokines in the sputum supernatant were measured by ELISA and Cytometric Bead Array. This is the first study to report the differential expression of different isoforms of IL-36 in different asthma phenotypes. IL-36α and IL-36β concentrations were significantly higher in the asthma group (P = 0.003 and 0.031), while IL-36Ra concentrations were significantly lower (P < 0.001) compared to healthy non-asthmatic controls. Sputum IL-36α and IL-36β concentrations in the neutrophilic asthma group were significantly higher than those in paucigranulocytic asthma (n = 24) and eosinophilic asthma groups (n = 23). IL-36α and IL-36β showed positive correlation with sputum neutrophils and total cell count (R = 0.689, P < 0.01; R = 0.304, P = 0.008; R = 0.689, P < 0.042; R = 0.253, P = 0.026). In conclusion, IL-36α and IL-36β may contribute to asthma airway inflammation by promoting neutrophil recruitment in airways. Our study provides insights into the inflammatory pathways of neutrophilic asthma and identifies potential therapeutic target. |
| first_indexed | 2024-03-08T14:14:51Z |
| format | Article |
| id | doaj.art-2085d73825cb4e89a0f15727d5062334 |
| institution | Directory Open Access Journal |
| issn | 1710-1492 |
| language | English |
| last_indexed | 2024-03-08T14:14:51Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Allergy, Asthma & Clinical Immunology |
| spelling | doaj.art-2085d73825cb4e89a0f15727d50623342024-01-14T12:26:51ZengBMCAllergy, Asthma & Clinical Immunology1710-14922024-01-0120111210.1186/s13223-023-00868-2Different expression levels of interleukin-36 in asthma phenotypesJinyan Li0Zhengda Wang1Hongna Dong2Yuqiu Hao3Peng Gao4Wei Li5Department of Respiratory and Critical Care Medicine, The Second Hospital of Jilin UniversityDepartment of Respiratory and Critical Care Medicine, The Second Hospital of Jilin UniversityDepartment of Respiratory and Critical Care Medicine, The Second Hospital of Jilin UniversityDepartment of Respiratory and Critical Care Medicine, The Second Hospital of Jilin UniversityDepartment of Respiratory and Critical Care Medicine, The Second Hospital of Jilin UniversityDepartment of Respiratory and Critical Care Medicine, The Second Hospital of Jilin UniversityAbstract Interleukin (IL)-36 family is closely associated with inflammation and consists of IL-36α, IL-36β, IL-36γ, and IL-36Ra. The role of IL-36 in the context of asthma and asthmatic phenotypes is not well characterized. We examined the sputum IL-36 levels in patients with different asthma phenotypes in order to unravel the mechanism of IL-36 in different asthma phenotypes. Our objective was to investigate the induced sputum IL-36α, IL-36β, IL-36γ, and IL-36Ra concentrations in patients with mild asthma, and to analyze the relationship of these markers with lung function and other cytokines in patients with different asthma phenotypes. Induced sputum samples were collected from patients with mild controlled asthma (n = 62, 27 males, age 54.77 ± 15.49) and healthy non-asthmatic controls (n = 16, 10 males, age 54.25 ± 14.60). Inflammatory cell counts in sputum were determined. The concentrations of IL-36 and other cytokines in the sputum supernatant were measured by ELISA and Cytometric Bead Array. This is the first study to report the differential expression of different isoforms of IL-36 in different asthma phenotypes. IL-36α and IL-36β concentrations were significantly higher in the asthma group (P = 0.003 and 0.031), while IL-36Ra concentrations were significantly lower (P < 0.001) compared to healthy non-asthmatic controls. Sputum IL-36α and IL-36β concentrations in the neutrophilic asthma group were significantly higher than those in paucigranulocytic asthma (n = 24) and eosinophilic asthma groups (n = 23). IL-36α and IL-36β showed positive correlation with sputum neutrophils and total cell count (R = 0.689, P < 0.01; R = 0.304, P = 0.008; R = 0.689, P < 0.042; R = 0.253, P = 0.026). In conclusion, IL-36α and IL-36β may contribute to asthma airway inflammation by promoting neutrophil recruitment in airways. Our study provides insights into the inflammatory pathways of neutrophilic asthma and identifies potential therapeutic target.https://doi.org/10.1186/s13223-023-00868-2AsthmaIL-36Induced sputumAsthma phenotypesAsthma patients |
| spellingShingle | Jinyan Li Zhengda Wang Hongna Dong Yuqiu Hao Peng Gao Wei Li Different expression levels of interleukin-36 in asthma phenotypes Allergy, Asthma & Clinical Immunology Asthma IL-36 Induced sputum Asthma phenotypes Asthma patients |
| title | Different expression levels of interleukin-36 in asthma phenotypes |
| title_full | Different expression levels of interleukin-36 in asthma phenotypes |
| title_fullStr | Different expression levels of interleukin-36 in asthma phenotypes |
| title_full_unstemmed | Different expression levels of interleukin-36 in asthma phenotypes |
| title_short | Different expression levels of interleukin-36 in asthma phenotypes |
| title_sort | different expression levels of interleukin 36 in asthma phenotypes |
| topic | Asthma IL-36 Induced sputum Asthma phenotypes Asthma patients |
| url | https://doi.org/10.1186/s13223-023-00868-2 |
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