Fast in vivo bioluminescence tomography using a novel pure optical imaging technique
Bioluminescence tomography (BLT) is a novel optical molecular imaging technique that advanced the conventional planar bioluminescence imaging (BLI) into a quantifiable three-dimensional (3D) approach in preclinical living animal studies in oncology. In order to solve the inverse problem and reconstr...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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World Scientific Publishing
2017-05-01
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| Series: | Journal of Innovative Optical Health Sciences |
| Subjects: | |
| Online Access: | http://www.worldscientific.com/doi/pdf/10.1142/S1793545817500031 |
| _version_ | 1819153928381530112 |
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| author | Shuang Zhang Chengcai Leng Hongbo Liu Kun Wang Jie Tian |
| author_facet | Shuang Zhang Chengcai Leng Hongbo Liu Kun Wang Jie Tian |
| author_sort | Shuang Zhang |
| collection | DOAJ |
| description | Bioluminescence tomography (BLT) is a novel optical molecular imaging technique that advanced the conventional planar bioluminescence imaging (BLI) into a quantifiable three-dimensional (3D) approach in preclinical living animal studies in oncology. In order to solve the inverse problem and reconstruct tumor lesions inside animal body accurately, the prior structural information is commonly obtained from X-ray computed tomography (CT). This strategy requires a complicated hybrid imaging system, extensive post imaging analysis and involvement of ionizing radiation. Moreover, the overall robustness highly depends on the fusion accuracy between the optical and structural information. Here, we present a pure optical bioluminescence tomographic (POBT) system and a novel BLT workflow based on multi-view projection acquisition and 3D surface reconstruction. This method can reconstruct the 3D surface of an imaging subject based on a sparse set of planar white-light and bioluminescent images, so that the prior structural information can be offered for 3D tumor lesion reconstruction without the involvement of CT. The performance of this novel technique was evaluated through the comparison with a conventional dual-modality tomographic (DMT) system and a commercialized optical imaging system (IVIS Spectrum) using three breast cancer xenografts. The results revealed that the new technique offered comparable in vivo tomographic accuracy with the DMT system (P>0.05) in much shorter data analysis time. It also offered significantly better accuracy comparing with the IVIS system (P<0.04) without sacrificing too much time. |
| first_indexed | 2024-12-22T15:12:59Z |
| format | Article |
| id | doaj.art-2086c0200f72407cb991f5755764a307 |
| institution | Directory Open Access Journal |
| issn | 1793-5458 1793-7205 |
| language | English |
| last_indexed | 2024-12-22T15:12:59Z |
| publishDate | 2017-05-01 |
| publisher | World Scientific Publishing |
| record_format | Article |
| series | Journal of Innovative Optical Health Sciences |
| spelling | doaj.art-2086c0200f72407cb991f5755764a3072022-12-21T18:21:49ZengWorld Scientific PublishingJournal of Innovative Optical Health Sciences1793-54581793-72052017-05-011031750003-11750003-1210.1142/S179354581750003110.1142/S1793545817500031Fast in vivo bioluminescence tomography using a novel pure optical imaging techniqueShuang Zhang0Chengcai Leng1Hongbo Liu2Kun Wang3Jie Tian4Sino-Dutch Biomedical and Information Engineering School, Northeastern University, Shenyang 110819, P. R. ChinaThe Key Laboratory of Molecular Imaging, Chinese Academy of Sciences, Zhongguancun East Road #95, Haidian Dist. Beijing 100190, P. R. ChinaThe Key Laboratory of Molecular Imaging, Chinese Academy of Sciences, Zhongguancun East Road #95, Haidian Dist. Beijing 100190, P. R. ChinaThe Key Laboratory of Molecular Imaging, Chinese Academy of Sciences, Zhongguancun East Road #95, Haidian Dist. Beijing 100190, P. R. ChinaThe Key Laboratory of Molecular Imaging, Chinese Academy of Sciences, Zhongguancun East Road #95, Haidian Dist. Beijing 100190, P. R. ChinaBioluminescence tomography (BLT) is a novel optical molecular imaging technique that advanced the conventional planar bioluminescence imaging (BLI) into a quantifiable three-dimensional (3D) approach in preclinical living animal studies in oncology. In order to solve the inverse problem and reconstruct tumor lesions inside animal body accurately, the prior structural information is commonly obtained from X-ray computed tomography (CT). This strategy requires a complicated hybrid imaging system, extensive post imaging analysis and involvement of ionizing radiation. Moreover, the overall robustness highly depends on the fusion accuracy between the optical and structural information. Here, we present a pure optical bioluminescence tomographic (POBT) system and a novel BLT workflow based on multi-view projection acquisition and 3D surface reconstruction. This method can reconstruct the 3D surface of an imaging subject based on a sparse set of planar white-light and bioluminescent images, so that the prior structural information can be offered for 3D tumor lesion reconstruction without the involvement of CT. The performance of this novel technique was evaluated through the comparison with a conventional dual-modality tomographic (DMT) system and a commercialized optical imaging system (IVIS Spectrum) using three breast cancer xenografts. The results revealed that the new technique offered comparable in vivo tomographic accuracy with the DMT system (P>0.05) in much shorter data analysis time. It also offered significantly better accuracy comparing with the IVIS system (P<0.04) without sacrificing too much time.http://www.worldscientific.com/doi/pdf/10.1142/S1793545817500031Optical surface reconstructionbioluminescence tomography reconstructionoptical molecular imaginglight flux reconstruction |
| spellingShingle | Shuang Zhang Chengcai Leng Hongbo Liu Kun Wang Jie Tian Fast in vivo bioluminescence tomography using a novel pure optical imaging technique Journal of Innovative Optical Health Sciences Optical surface reconstruction bioluminescence tomography reconstruction optical molecular imaging light flux reconstruction |
| title | Fast in vivo bioluminescence tomography using a novel pure optical imaging technique |
| title_full | Fast in vivo bioluminescence tomography using a novel pure optical imaging technique |
| title_fullStr | Fast in vivo bioluminescence tomography using a novel pure optical imaging technique |
| title_full_unstemmed | Fast in vivo bioluminescence tomography using a novel pure optical imaging technique |
| title_short | Fast in vivo bioluminescence tomography using a novel pure optical imaging technique |
| title_sort | fast in vivo bioluminescence tomography using a novel pure optical imaging technique |
| topic | Optical surface reconstruction bioluminescence tomography reconstruction optical molecular imaging light flux reconstruction |
| url | http://www.worldscientific.com/doi/pdf/10.1142/S1793545817500031 |
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