An “on-off-on” type photoelectrochemical aptasensor for ultrasensitive detecting prostate-specific antigen based on resonance energy transfer
An “on-off-on” type photoelectrochemical (PEC) aptamer sensor for prostate-specific antigen (PSA) detection is constructed based on nanocomposite materials obtained from CdS functionalized titanium dioxide (CdS/TiO2 NPs). Firstly, due to high photocurrent signal of the CdS/TiO2 NPs, it can achieve t...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Biosensors and Bioelectronics: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590137022000255 |
| _version_ | 1811244618880122880 |
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| author | Jiuying Cui Qingfu Guo Jingjing Wang Ya Tian Guangming Nie |
| author_facet | Jiuying Cui Qingfu Guo Jingjing Wang Ya Tian Guangming Nie |
| author_sort | Jiuying Cui |
| collection | DOAJ |
| description | An “on-off-on” type photoelectrochemical (PEC) aptamer sensor for prostate-specific antigen (PSA) detection is constructed based on nanocomposite materials obtained from CdS functionalized titanium dioxide (CdS/TiO2 NPs). Firstly, due to high photocurrent signal of the CdS/TiO2 NPs, it can achieve the initial signal “on” state to improve the stability of the sensing platform. Then, AuNPs, as the energy acceptor of CdS, are used to label PSA aptamers to obtain signal “off” state through energy resonance transfer (RET). Finally, in the process of detecting PSA, part of the AuNPs-modified aptamer detaches from the electrode surface, causing the sensor signal to “on” state again. This stepped energy level structure formed by the coupling of CdS and TiO2 NPs can broaden the light absorption range, enhance electron-hole separation, and lay a good foundation for the signal “on-off-on” strategy. The method on RET to realize signal quenching and opening significantly improves the sensitivity of the PEC sensor. Based on this innovative “on-off-on” design, this PEC aptamer sensor has high sensitivity to PSA, and its linear detection range (LDR) is 0.0005–50 ng mL−1. The limit of detection (LOD) is 0.00017 ng mL−1. This constructed PEC sensor also has good detection performances in actual human serum sample detection. And this study based on RET can also provide a new strategy for the detection of other biomarkers. |
| first_indexed | 2024-04-12T14:27:14Z |
| format | Article |
| id | doaj.art-208bae529604446894e5e8512f44cabe |
| institution | Directory Open Access Journal |
| issn | 2590-1370 |
| language | English |
| last_indexed | 2024-04-12T14:27:14Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biosensors and Bioelectronics: X |
| spelling | doaj.art-208bae529604446894e5e8512f44cabe2022-12-22T03:29:23ZengElsevierBiosensors and Bioelectronics: X2590-13702022-05-0110100130An “on-off-on” type photoelectrochemical aptasensor for ultrasensitive detecting prostate-specific antigen based on resonance energy transferJiuying Cui0Qingfu Guo1Jingjing Wang2Ya Tian3Guangming Nie4Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, ChinaKey Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, ChinaKey Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, ChinaKey Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, ChinaCorresponding author.; Key Laboratory of Optic-Electric Sensing and Analytical Chemistry for Life Science, MOE, State Key Laboratory Base of Eco-chemical Engineering, College of Chemistry and Molecular Engineering, Qingdao University of Science and Technology, Qingdao, 266042, ChinaAn “on-off-on” type photoelectrochemical (PEC) aptamer sensor for prostate-specific antigen (PSA) detection is constructed based on nanocomposite materials obtained from CdS functionalized titanium dioxide (CdS/TiO2 NPs). Firstly, due to high photocurrent signal of the CdS/TiO2 NPs, it can achieve the initial signal “on” state to improve the stability of the sensing platform. Then, AuNPs, as the energy acceptor of CdS, are used to label PSA aptamers to obtain signal “off” state through energy resonance transfer (RET). Finally, in the process of detecting PSA, part of the AuNPs-modified aptamer detaches from the electrode surface, causing the sensor signal to “on” state again. This stepped energy level structure formed by the coupling of CdS and TiO2 NPs can broaden the light absorption range, enhance electron-hole separation, and lay a good foundation for the signal “on-off-on” strategy. The method on RET to realize signal quenching and opening significantly improves the sensitivity of the PEC sensor. Based on this innovative “on-off-on” design, this PEC aptamer sensor has high sensitivity to PSA, and its linear detection range (LDR) is 0.0005–50 ng mL−1. The limit of detection (LOD) is 0.00017 ng mL−1. This constructed PEC sensor also has good detection performances in actual human serum sample detection. And this study based on RET can also provide a new strategy for the detection of other biomarkers.http://www.sciencedirect.com/science/article/pii/S2590137022000255PhotoelectrochemistryEnergy resonance transferAptamer sensorProstate-specific antigenTitanium dioxideCadmium sulfide |
| spellingShingle | Jiuying Cui Qingfu Guo Jingjing Wang Ya Tian Guangming Nie An “on-off-on” type photoelectrochemical aptasensor for ultrasensitive detecting prostate-specific antigen based on resonance energy transfer Biosensors and Bioelectronics: X Photoelectrochemistry Energy resonance transfer Aptamer sensor Prostate-specific antigen Titanium dioxide Cadmium sulfide |
| title | An “on-off-on” type photoelectrochemical aptasensor for ultrasensitive detecting prostate-specific antigen based on resonance energy transfer |
| title_full | An “on-off-on” type photoelectrochemical aptasensor for ultrasensitive detecting prostate-specific antigen based on resonance energy transfer |
| title_fullStr | An “on-off-on” type photoelectrochemical aptasensor for ultrasensitive detecting prostate-specific antigen based on resonance energy transfer |
| title_full_unstemmed | An “on-off-on” type photoelectrochemical aptasensor for ultrasensitive detecting prostate-specific antigen based on resonance energy transfer |
| title_short | An “on-off-on” type photoelectrochemical aptasensor for ultrasensitive detecting prostate-specific antigen based on resonance energy transfer |
| title_sort | on off on type photoelectrochemical aptasensor for ultrasensitive detecting prostate specific antigen based on resonance energy transfer |
| topic | Photoelectrochemistry Energy resonance transfer Aptamer sensor Prostate-specific antigen Titanium dioxide Cadmium sulfide |
| url | http://www.sciencedirect.com/science/article/pii/S2590137022000255 |
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