In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae.
Intracellular biochemical parameters, such as the expression level of gene products, are considered to be optimized so that a biological system, including the parameters, works effectively. Those parameters should have some permissible range so that the systems have robustness against perturbations,...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2006-07-01
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| Series: | PLoS Genetics |
| Online Access: | http://europepmc.org/articles/PMC1500812?pdf=render |
| _version_ | 1818155864665620480 |
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| author | Hisao Moriya Yuki Shimizu-Yoshida Hiroaki Kitano |
| author_facet | Hisao Moriya Yuki Shimizu-Yoshida Hiroaki Kitano |
| author_sort | Hisao Moriya |
| collection | DOAJ |
| description | Intracellular biochemical parameters, such as the expression level of gene products, are considered to be optimized so that a biological system, including the parameters, works effectively. Those parameters should have some permissible range so that the systems have robustness against perturbations, such as noise in gene expression. However, little is known about the permissible range in real cells because there has been no experimental technique to test it. In this study, we developed a genetic screening method, named "genetic tug-of-war" (gTOW) that evaluates upper limit copy numbers of genes in a model eukaryote Saccharomyces cerevisiae, and we applied it for 30 cell-cycle related genes (CDC genes). The experiment provided unique quantitative data that could be used to argue the system-level properties of the cell cycle such as robustness and fragility. The data were used to evaluate the current computational model, and refinements to the model were suggested. |
| first_indexed | 2024-12-11T14:49:11Z |
| format | Article |
| id | doaj.art-2090c0f21a08424baf9f788867526a53 |
| institution | Directory Open Access Journal |
| issn | 1553-7390 1553-7404 |
| language | English |
| last_indexed | 2024-12-11T14:49:11Z |
| publishDate | 2006-07-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Genetics |
| spelling | doaj.art-2090c0f21a08424baf9f788867526a532022-12-22T01:01:33ZengPublic Library of Science (PLoS)PLoS Genetics1553-73901553-74042006-07-0127e11110.1371/journal.pgen.0020111In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae.Hisao MoriyaYuki Shimizu-YoshidaHiroaki KitanoIntracellular biochemical parameters, such as the expression level of gene products, are considered to be optimized so that a biological system, including the parameters, works effectively. Those parameters should have some permissible range so that the systems have robustness against perturbations, such as noise in gene expression. However, little is known about the permissible range in real cells because there has been no experimental technique to test it. In this study, we developed a genetic screening method, named "genetic tug-of-war" (gTOW) that evaluates upper limit copy numbers of genes in a model eukaryote Saccharomyces cerevisiae, and we applied it for 30 cell-cycle related genes (CDC genes). The experiment provided unique quantitative data that could be used to argue the system-level properties of the cell cycle such as robustness and fragility. The data were used to evaluate the current computational model, and refinements to the model were suggested.http://europepmc.org/articles/PMC1500812?pdf=render |
| spellingShingle | Hisao Moriya Yuki Shimizu-Yoshida Hiroaki Kitano In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae. PLoS Genetics |
| title | In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae. |
| title_full | In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae. |
| title_fullStr | In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae. |
| title_full_unstemmed | In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae. |
| title_short | In vivo robustness analysis of cell division cycle genes in Saccharomyces cerevisiae. |
| title_sort | in vivo robustness analysis of cell division cycle genes in saccharomyces cerevisiae |
| url | http://europepmc.org/articles/PMC1500812?pdf=render |
| work_keys_str_mv | AT hisaomoriya invivorobustnessanalysisofcelldivisioncyclegenesinsaccharomycescerevisiae AT yukishimizuyoshida invivorobustnessanalysisofcelldivisioncyclegenesinsaccharomycescerevisiae AT hiroakikitano invivorobustnessanalysisofcelldivisioncyclegenesinsaccharomycescerevisiae |