Protocol to isolate mature thymic T cell subsets using fluorescence-activated cell sorting for assessing gene expression by RNA-seq and transcription factor binding across the genome by CUT&RUN

Protocol to isolate mature thymic T cell subsets using fluorescence-activated cell sorting for assessing gene expression by RNA-seq and transcription factor binding across the genome by CUT&RUN

Summary: Here, we describe steps to isolate mature thymic T cell subsets, namely CD4 single positive (SP), CD8 SP, and invariant natural killer T (iNKT) cells starting from murine total thymocytes using fluorescence-activated cell sorting. We detail protocols to study gene expression by RNA-seq and...

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Bibliographic Details
Main Authors: Dimitris Theofilatos, Tarmo Äijö, Ageliki Tsagaratou
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Cell isolation
Flow Cytometry/Mass Cytometry
RNAseq
ChIPseq
Immunology
Gene Expression
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007195

Internet

http://www.sciencedirect.com/science/article/pii/S2666166722007195

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