MLL rearrangements in pediatric acute lymphoblastic and myeloblastic leukemias: MLL specific and lineage specific signatures
<p>Abstract</p> <p>Background</p> <p>The presence of <it>MLL </it>rearrangements in acute leukemia results in a complex number of biological modifications that still remain largely unexplained. Armstrong et al. proposed <it>MLL </it>rearrangement...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2009-06-01
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| Series: | BMC Medical Genomics |
| Online Access: | http://www.biomedcentral.com/1755-8794/2/36 |
| _version_ | 1811189368088428544 |
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| author | te Kronnie Geertruy Dell'Orto Marta Zangrando Andrea Basso Giuseppe |
| author_facet | te Kronnie Geertruy Dell'Orto Marta Zangrando Andrea Basso Giuseppe |
| author_sort | te Kronnie Geertruy |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The presence of <it>MLL </it>rearrangements in acute leukemia results in a complex number of biological modifications that still remain largely unexplained. Armstrong et al. proposed <it>MLL </it>rearrangement positive ALL as a distinct subgroup, separated from acute lymphoblastic (ALL) and myeloblastic leukemia (AML), with a specific gene expression profile. Here we show that MLL, from both ALL and AML origin, share a signature identified by a small set of genes suggesting a common genetic disregulation that could be at the basis of mixed lineage leukemia in both phenotypes.</p> <p>Methods</p> <p>Using Affymetrix<sup>® </sup>HG-U133 Plus 2.0 platform, gene expression data from 140 (training set) + 78 (test set) ALL and AML patients with (24+13) and without (116+65) <it>MLL </it>rearrangements have been investigated performing class comparison (SAM) and class prediction (PAM) analyses.</p> <p>Results</p> <p>We identified a <it>MLL </it>translocation-specific (379 probes) signature and a phenotype-specific (622 probes) signature which have been tested using unsupervised methods. A final subset of 14 genes grants the characterization of acute leukemia patients with and without <it>MLL </it>rearrangements.</p> <p>Conclusion</p> <p>Our study demonstrated that a small subset of genes identifies <it>MLL</it>-specific rearrangements and clearly separates acute leukemia samples according to lineage origin. The subset included well-known genes and newly discovered markers that identified ALL and AML subgroups, with and without <it>MLL </it>rearrangements.</p> |
| first_indexed | 2024-04-11T14:33:50Z |
| format | Article |
| id | doaj.art-20926b4a8d874777a4d3eff08b4f9931 |
| institution | Directory Open Access Journal |
| issn | 1755-8794 |
| language | English |
| last_indexed | 2024-04-11T14:33:50Z |
| publishDate | 2009-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Medical Genomics |
| spelling | doaj.art-20926b4a8d874777a4d3eff08b4f99312022-12-22T04:18:25ZengBMCBMC Medical Genomics1755-87942009-06-01213610.1186/1755-8794-2-36MLL rearrangements in pediatric acute lymphoblastic and myeloblastic leukemias: MLL specific and lineage specific signatureste Kronnie GeertruyDell'Orto MartaZangrando AndreaBasso Giuseppe<p>Abstract</p> <p>Background</p> <p>The presence of <it>MLL </it>rearrangements in acute leukemia results in a complex number of biological modifications that still remain largely unexplained. Armstrong et al. proposed <it>MLL </it>rearrangement positive ALL as a distinct subgroup, separated from acute lymphoblastic (ALL) and myeloblastic leukemia (AML), with a specific gene expression profile. Here we show that MLL, from both ALL and AML origin, share a signature identified by a small set of genes suggesting a common genetic disregulation that could be at the basis of mixed lineage leukemia in both phenotypes.</p> <p>Methods</p> <p>Using Affymetrix<sup>® </sup>HG-U133 Plus 2.0 platform, gene expression data from 140 (training set) + 78 (test set) ALL and AML patients with (24+13) and without (116+65) <it>MLL </it>rearrangements have been investigated performing class comparison (SAM) and class prediction (PAM) analyses.</p> <p>Results</p> <p>We identified a <it>MLL </it>translocation-specific (379 probes) signature and a phenotype-specific (622 probes) signature which have been tested using unsupervised methods. A final subset of 14 genes grants the characterization of acute leukemia patients with and without <it>MLL </it>rearrangements.</p> <p>Conclusion</p> <p>Our study demonstrated that a small subset of genes identifies <it>MLL</it>-specific rearrangements and clearly separates acute leukemia samples according to lineage origin. The subset included well-known genes and newly discovered markers that identified ALL and AML subgroups, with and without <it>MLL </it>rearrangements.</p>http://www.biomedcentral.com/1755-8794/2/36 |
| spellingShingle | te Kronnie Geertruy Dell'Orto Marta Zangrando Andrea Basso Giuseppe MLL rearrangements in pediatric acute lymphoblastic and myeloblastic leukemias: MLL specific and lineage specific signatures BMC Medical Genomics |
| title | MLL rearrangements in pediatric acute lymphoblastic and myeloblastic leukemias: MLL specific and lineage specific signatures |
| title_full | MLL rearrangements in pediatric acute lymphoblastic and myeloblastic leukemias: MLL specific and lineage specific signatures |
| title_fullStr | MLL rearrangements in pediatric acute lymphoblastic and myeloblastic leukemias: MLL specific and lineage specific signatures |
| title_full_unstemmed | MLL rearrangements in pediatric acute lymphoblastic and myeloblastic leukemias: MLL specific and lineage specific signatures |
| title_short | MLL rearrangements in pediatric acute lymphoblastic and myeloblastic leukemias: MLL specific and lineage specific signatures |
| title_sort | mll rearrangements in pediatric acute lymphoblastic and myeloblastic leukemias mll specific and lineage specific signatures |
| url | http://www.biomedcentral.com/1755-8794/2/36 |
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