The Origins of Salivary Vitamin A, Vitamin B<sub>12</sub> and Vitamin D-Binding Proteins
Vitamin A- (retinol), vitamin B<sub>12</sub>- (haptocorrin) and vitamin D-binding proteins are the major circulatory transporters of their respective ligands; they are also constituents of the salivary proteome, the origins of which, remain unclear. The aim of this study was to explore h...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-12-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/12/12/3838 |
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| author | Matthew Blakeley Agata Sobczyńska-Malefora Guy Carpenter |
| author_facet | Matthew Blakeley Agata Sobczyńska-Malefora Guy Carpenter |
| author_sort | Matthew Blakeley |
| collection | DOAJ |
| description | Vitamin A- (retinol), vitamin B<sub>12</sub>- (haptocorrin) and vitamin D-binding proteins are the major circulatory transporters of their respective ligands; they are also constituents of the salivary proteome, the origins of which, remain unclear. The aim of this study was to explore how these proteins enter saliva and their relationship (if any) with vitamin status. Firstly, the three vitamin-binding proteins were quantified in resting whole mouth saliva and chewing-stimulated saliva from healthy donors (<i>n</i> = 10) to determine if they enter the mouth by salivary secretion or from the circulation. Secondly paired whole mouth saliva and serum samples were analysed from healthy donors (<i>n</i> = 14) to determine the relationships between the vitamin-binding proteins and vitamin status. Salivary output of all three vitamin-binding proteins studied increased when secretion was stimulated, suggesting they are secreted by the salivary glands. Whilst retinol-binding protein and haptocorrin were secreted by all major salivary glands, vitamin D-binding protein was restricted to the mucus glands. Salivary vitamin-binding protein concentrations were not found to be indicative of systemic vitamin status. |
| first_indexed | 2024-03-10T14:01:46Z |
| format | Article |
| id | doaj.art-209a0ca028c0469e9c298efbde5d4de6 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T14:01:46Z |
| publishDate | 2020-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-209a0ca028c0469e9c298efbde5d4de62023-11-21T00:59:37ZengMDPI AGNutrients2072-66432020-12-011212383810.3390/nu12123838The Origins of Salivary Vitamin A, Vitamin B<sub>12</sub> and Vitamin D-Binding ProteinsMatthew Blakeley0Agata Sobczyńska-Malefora1Guy Carpenter2Salivary Research, Centre for Host-Microbiome Interactions, Faculty of Dental, Oral and Craniofacial Sciences (FoDOCS), King’s College London, London SE1 1UL, UKThe Nutristasis Unit, Thomas’ Hospital, Viapath, St., London SE1 7EH, UKSalivary Research, Centre for Host-Microbiome Interactions, Faculty of Dental, Oral and Craniofacial Sciences (FoDOCS), King’s College London, London SE1 1UL, UKVitamin A- (retinol), vitamin B<sub>12</sub>- (haptocorrin) and vitamin D-binding proteins are the major circulatory transporters of their respective ligands; they are also constituents of the salivary proteome, the origins of which, remain unclear. The aim of this study was to explore how these proteins enter saliva and their relationship (if any) with vitamin status. Firstly, the three vitamin-binding proteins were quantified in resting whole mouth saliva and chewing-stimulated saliva from healthy donors (<i>n</i> = 10) to determine if they enter the mouth by salivary secretion or from the circulation. Secondly paired whole mouth saliva and serum samples were analysed from healthy donors (<i>n</i> = 14) to determine the relationships between the vitamin-binding proteins and vitamin status. Salivary output of all three vitamin-binding proteins studied increased when secretion was stimulated, suggesting they are secreted by the salivary glands. Whilst retinol-binding protein and haptocorrin were secreted by all major salivary glands, vitamin D-binding protein was restricted to the mucus glands. Salivary vitamin-binding protein concentrations were not found to be indicative of systemic vitamin status.https://www.mdpi.com/2072-6643/12/12/3838vitaminssalivahaptocorrinvitamin D-binding proteinretinol-binding protein |
| spellingShingle | Matthew Blakeley Agata Sobczyńska-Malefora Guy Carpenter The Origins of Salivary Vitamin A, Vitamin B<sub>12</sub> and Vitamin D-Binding Proteins Nutrients vitamins saliva haptocorrin vitamin D-binding protein retinol-binding protein |
| title | The Origins of Salivary Vitamin A, Vitamin B<sub>12</sub> and Vitamin D-Binding Proteins |
| title_full | The Origins of Salivary Vitamin A, Vitamin B<sub>12</sub> and Vitamin D-Binding Proteins |
| title_fullStr | The Origins of Salivary Vitamin A, Vitamin B<sub>12</sub> and Vitamin D-Binding Proteins |
| title_full_unstemmed | The Origins of Salivary Vitamin A, Vitamin B<sub>12</sub> and Vitamin D-Binding Proteins |
| title_short | The Origins of Salivary Vitamin A, Vitamin B<sub>12</sub> and Vitamin D-Binding Proteins |
| title_sort | origins of salivary vitamin a vitamin b sub 12 sub and vitamin d binding proteins |
| topic | vitamins saliva haptocorrin vitamin D-binding protein retinol-binding protein |
| url | https://www.mdpi.com/2072-6643/12/12/3838 |
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