The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update
Organoids represent one of the most important advancements in the field of stem cells during the past decade. They are three-dimensional in vitro culturing models that originate from self-organizing stem cells and can mimic the in vivo structural and functional specificities of body organs. Organoid...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-07-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/22/14/7667 |
| _version_ | 1797526907771682816 |
|---|---|
| author | Joseph Azar Hisham F. Bahmad Darine Daher Maya M. Moubarak Ola Hadadeh Alissar Monzer Samar Al Bitar Mohamed Jamal Mohamed Al-Sayegh Wassim Abou-Kheir |
| author_facet | Joseph Azar Hisham F. Bahmad Darine Daher Maya M. Moubarak Ola Hadadeh Alissar Monzer Samar Al Bitar Mohamed Jamal Mohamed Al-Sayegh Wassim Abou-Kheir |
| author_sort | Joseph Azar |
| collection | DOAJ |
| description | Organoids represent one of the most important advancements in the field of stem cells during the past decade. They are three-dimensional in vitro culturing models that originate from self-organizing stem cells and can mimic the in vivo structural and functional specificities of body organs. Organoids have been established from multiple adult tissues as well as pluripotent stem cells and have recently become a powerful tool for studying development and diseases in vitro, drug screening, and host–microbe interaction. The use of stem cells—that have self-renewal capacity to proliferate and differentiate into specialized cell types—for organoids culturing represents a major advancement in biomedical research. Indeed, this new technology has a great potential to be used in a multitude of fields, including cancer research, hereditary and infectious diseases. Nevertheless, organoid culturing is still rife with many challenges, not limited to being costly and time consuming, having variable rates of efficiency in generation and maintenance, genetic stability, and clinical applications. In this review, we aim to provide a synopsis of pluripotent stem cell-derived organoids and their use for disease modeling and other clinical applications. |
| first_indexed | 2024-03-10T09:36:01Z |
| format | Article |
| id | doaj.art-209b2048ac3f4f7194f312add9c80acc |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T09:36:01Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-209b2048ac3f4f7194f312add9c80acc2023-11-22T04:02:17ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-07-012214766710.3390/ijms22147667The Use of Stem Cell-Derived Organoids in Disease Modeling: An UpdateJoseph Azar0Hisham F. Bahmad1Darine Daher2Maya M. Moubarak3Ola Hadadeh4Alissar Monzer5Samar Al Bitar6Mohamed Jamal7Mohamed Al-Sayegh8Wassim Abou-Kheir9Department of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2260, LebanonDepartment of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2260, LebanonDepartment of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2260, LebanonDepartment of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2260, LebanonDepartment of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2260, LebanonDepartment of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2260, LebanonDepartment of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2260, LebanonHamdan Bin Mohammed College of Dental Medicine, Mohammed Bin Rashid University of Medicine and Health Sciences, Dubai 66566, United Arab EmiratesBiology Division, New York University Abu Dhabi, Abu Dhabi 2460, United Arab EmiratesDepartment of Anatomy, Cell Biology and Physiological Sciences, Faculty of Medicine, American University of Beirut, Beirut 1107 2260, LebanonOrganoids represent one of the most important advancements in the field of stem cells during the past decade. They are three-dimensional in vitro culturing models that originate from self-organizing stem cells and can mimic the in vivo structural and functional specificities of body organs. Organoids have been established from multiple adult tissues as well as pluripotent stem cells and have recently become a powerful tool for studying development and diseases in vitro, drug screening, and host–microbe interaction. The use of stem cells—that have self-renewal capacity to proliferate and differentiate into specialized cell types—for organoids culturing represents a major advancement in biomedical research. Indeed, this new technology has a great potential to be used in a multitude of fields, including cancer research, hereditary and infectious diseases. Nevertheless, organoid culturing is still rife with many challenges, not limited to being costly and time consuming, having variable rates of efficiency in generation and maintenance, genetic stability, and clinical applications. In this review, we aim to provide a synopsis of pluripotent stem cell-derived organoids and their use for disease modeling and other clinical applications.https://www.mdpi.com/1422-0067/22/14/7667pluripotent stem cellsembryonic stem cellsorganoidsdisease modeling3D culturing |
| spellingShingle | Joseph Azar Hisham F. Bahmad Darine Daher Maya M. Moubarak Ola Hadadeh Alissar Monzer Samar Al Bitar Mohamed Jamal Mohamed Al-Sayegh Wassim Abou-Kheir The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update International Journal of Molecular Sciences pluripotent stem cells embryonic stem cells organoids disease modeling 3D culturing |
| title | The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update |
| title_full | The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update |
| title_fullStr | The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update |
| title_full_unstemmed | The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update |
| title_short | The Use of Stem Cell-Derived Organoids in Disease Modeling: An Update |
| title_sort | use of stem cell derived organoids in disease modeling an update |
| topic | pluripotent stem cells embryonic stem cells organoids disease modeling 3D culturing |
| url | https://www.mdpi.com/1422-0067/22/14/7667 |
| work_keys_str_mv | AT josephazar theuseofstemcellderivedorganoidsindiseasemodelinganupdate AT hishamfbahmad theuseofstemcellderivedorganoidsindiseasemodelinganupdate AT darinedaher theuseofstemcellderivedorganoidsindiseasemodelinganupdate AT mayammoubarak theuseofstemcellderivedorganoidsindiseasemodelinganupdate AT olahadadeh theuseofstemcellderivedorganoidsindiseasemodelinganupdate AT alissarmonzer theuseofstemcellderivedorganoidsindiseasemodelinganupdate AT samaralbitar theuseofstemcellderivedorganoidsindiseasemodelinganupdate AT mohamedjamal theuseofstemcellderivedorganoidsindiseasemodelinganupdate AT mohamedalsayegh theuseofstemcellderivedorganoidsindiseasemodelinganupdate AT wassimaboukheir theuseofstemcellderivedorganoidsindiseasemodelinganupdate AT josephazar useofstemcellderivedorganoidsindiseasemodelinganupdate AT hishamfbahmad useofstemcellderivedorganoidsindiseasemodelinganupdate AT darinedaher useofstemcellderivedorganoidsindiseasemodelinganupdate AT mayammoubarak useofstemcellderivedorganoidsindiseasemodelinganupdate AT olahadadeh useofstemcellderivedorganoidsindiseasemodelinganupdate AT alissarmonzer useofstemcellderivedorganoidsindiseasemodelinganupdate AT samaralbitar useofstemcellderivedorganoidsindiseasemodelinganupdate AT mohamedjamal useofstemcellderivedorganoidsindiseasemodelinganupdate AT mohamedalsayegh useofstemcellderivedorganoidsindiseasemodelinganupdate AT wassimaboukheir useofstemcellderivedorganoidsindiseasemodelinganupdate |