Pterin determination in cerebrospinal fluid: state of the art
The analysis of pterins in the cerebrospinal fluid (CSF) is mandatory for the etiologic diagnosis of inborn errors of dopamine and serotonin metabolism. The success of the available therapeutic strategies for preventing the ongoing brain dysfunction is tightly dependent of the early diagnosis of the...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
De Gruyter
2017-08-01
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| Series: | Pteridines |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/pterid-2017-0001 |
| _version_ | 1818737988535844864 |
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| author | Guibal Pierre Lo Aurélien Maitre Philippe Moussa Fathi |
| author_facet | Guibal Pierre Lo Aurélien Maitre Philippe Moussa Fathi |
| author_sort | Guibal Pierre |
| collection | DOAJ |
| description | The analysis of pterins in the cerebrospinal fluid (CSF) is mandatory for the etiologic diagnosis of inborn errors of dopamine and serotonin metabolism. The success of the available therapeutic strategies for preventing the ongoing brain dysfunction is tightly dependent of the early diagnosis of these neurotransmitter disorders. Previous methods of pterins determination in the CSF have in common at least one reversed phase HPLC step coupled to electrochemical or fluorescence detection (FD). They differ in the oxidation procedure of the reduced forms of pterins into their oxidized fluorescent counterparts. Most of the methods using the FD include at least one offline chemical oxidation procedure and cannot allow the direct quantification of tetrahydrobiopterin (BH4). A recent method proposed a single step simultaneous quantification of all forms of pterins including BH4 by HPLC coupled to FD after post-column coulometric oxidation. Nowadays, recent advances in mass spectrometry (MS), notably in term of sensitivity, allow the direct unambiguous determination of all forms of pterins in the CSF by LC-MS/MS. |
| first_indexed | 2024-12-18T01:01:47Z |
| format | Article |
| id | doaj.art-209b353a56ec4a838378facfe3fed740 |
| institution | Directory Open Access Journal |
| issn | 0933-4807 2195-4720 |
| language | English |
| last_indexed | 2024-12-18T01:01:47Z |
| publishDate | 2017-08-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Pteridines |
| spelling | doaj.art-209b353a56ec4a838378facfe3fed7402022-12-21T21:26:21ZengDe GruyterPteridines0933-48072195-47202017-08-01282838910.1515/pterid-2017-0001Pterin determination in cerebrospinal fluid: state of the artGuibal Pierre0Lo Aurélien1Maitre Philippe2Moussa Fathi3Letiam, Lip(Sys)2, Université Paris-Sud, IUT d’Orsay, Plateau de Moulon, 91405 Orsay, FranceLetiam, Lip(Sys)2, Université Paris-Sud, IUT d’Orsay, Plateau de Moulon, 91405 Orsay, FranceLaboratoire de Chimie Physique, UMR 8000 CNRS, Faculté des Sciences d’Orsay, Université Paris-Sud, 15, Rue G. Clemenceau, 91400 Orsay, FranceLetiam, Lip(Sys)2, Université Paris-Sud, IUT d’Orsay, Plateau de Moulon, 91405 Orsay, FranceThe analysis of pterins in the cerebrospinal fluid (CSF) is mandatory for the etiologic diagnosis of inborn errors of dopamine and serotonin metabolism. The success of the available therapeutic strategies for preventing the ongoing brain dysfunction is tightly dependent of the early diagnosis of these neurotransmitter disorders. Previous methods of pterins determination in the CSF have in common at least one reversed phase HPLC step coupled to electrochemical or fluorescence detection (FD). They differ in the oxidation procedure of the reduced forms of pterins into their oxidized fluorescent counterparts. Most of the methods using the FD include at least one offline chemical oxidation procedure and cannot allow the direct quantification of tetrahydrobiopterin (BH4). A recent method proposed a single step simultaneous quantification of all forms of pterins including BH4 by HPLC coupled to FD after post-column coulometric oxidation. Nowadays, recent advances in mass spectrometry (MS), notably in term of sensitivity, allow the direct unambiguous determination of all forms of pterins in the CSF by LC-MS/MS.https://doi.org/10.1515/pterid-2017-0001biopterincerebrospinal fluidneopterinneurological disordersneurotransmitters |
| spellingShingle | Guibal Pierre Lo Aurélien Maitre Philippe Moussa Fathi Pterin determination in cerebrospinal fluid: state of the art Pteridines biopterin cerebrospinal fluid neopterin neurological disorders neurotransmitters |
| title | Pterin determination in cerebrospinal fluid: state of the art |
| title_full | Pterin determination in cerebrospinal fluid: state of the art |
| title_fullStr | Pterin determination in cerebrospinal fluid: state of the art |
| title_full_unstemmed | Pterin determination in cerebrospinal fluid: state of the art |
| title_short | Pterin determination in cerebrospinal fluid: state of the art |
| title_sort | pterin determination in cerebrospinal fluid state of the art |
| topic | biopterin cerebrospinal fluid neopterin neurological disorders neurotransmitters |
| url | https://doi.org/10.1515/pterid-2017-0001 |
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