Magnesium Oxide Nanoparticles: An Influential Element in Cowpea (<i>Vigna unguiculata</i> L. Walp.) Tissue Culture
Nanotechnology is a rapidly growing field of science and technology that deals with the development of new solutions by understanding and controlling matter at the nanoscale. Since the last decade, magnesium oxide nanoparticles (MgO-NPs) have gained tremendous attention because of their unique chara...
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MDPI AG
2023-06-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/13/6/1646 |
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| author | Rabia Koçak Melih Okcu Kamil Haliloğlu Aras Türkoğlu Alireza Pour-Aboughadareh Bita Jamshidi Tibor Janda Azize Alaylı Hayrunnisa Nadaroğlu |
| author_facet | Rabia Koçak Melih Okcu Kamil Haliloğlu Aras Türkoğlu Alireza Pour-Aboughadareh Bita Jamshidi Tibor Janda Azize Alaylı Hayrunnisa Nadaroğlu |
| author_sort | Rabia Koçak |
| collection | DOAJ |
| description | Nanotechnology is a rapidly growing field of science and technology that deals with the development of new solutions by understanding and controlling matter at the nanoscale. Since the last decade, magnesium oxide nanoparticles (MgO-NPs) have gained tremendous attention because of their unique characteristics and diverse applications in materials sciences and because they are non-toxic and relatively cheaply available materials. MgO-NPs can improve plant growth and contribute to plant tolerance of heavy metal toxicity. The effects of MgO-NPs on cowpea (<i>Vigna unguiculata</i> L. Walp.) plants were surveyed under in vitro conditions to find the optimum combination for cowpea tissue culture. The MgO-NPs used in the study were synthesized using walnut shell extract by the green synthesis method. MgO nanoparticles with 35–40 nm size was used in this research. When the size distribution of the MgO-NPs’ structure was examined, two peaks with 37.8 nm and 78.8 nm dimensions were obtained. The zeta potential of MgO-NPs dispersed in water was measured around −13.3 mV on average. The results showed that different doses of MgO-NPs applied to cowpea plant on all in vitro parameters significantly affected all measured parameters of cowpea plantlets under in vitro condition in a positive way. The best results in morphogenesis were MS medium supplemented with high MgO-NP applications (555 mg/L), resulting in a 25% increase in callus formation. The addition of Mg-NPs in the induction medium at concentrations at 370 mg/L increased shoot multiplication. The highest root length with 1.575 cm was obtained in MS medium containing 370 mg/L MgO. This study found that MgO-NPs greatly influenced the plantlets’ growth parameters and other measured traits; in addition, our results indicate that the efficiency of tissue culture of cowpea could be improved by increased application of MgO in the form of nanoparticles. In conclusion, the present work highlights the possibility of using MgO-NPs in cowpea tissue culture. |
| first_indexed | 2024-03-11T02:51:29Z |
| format | Article |
| id | doaj.art-20a3091caeb44d0ea2b6793d506619d1 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-11T02:51:29Z |
| publishDate | 2023-06-01 |
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| series | Agronomy |
| spelling | doaj.art-20a3091caeb44d0ea2b6793d506619d12023-11-18T08:56:06ZengMDPI AGAgronomy2073-43952023-06-01136164610.3390/agronomy13061646Magnesium Oxide Nanoparticles: An Influential Element in Cowpea (<i>Vigna unguiculata</i> L. Walp.) Tissue CultureRabia Koçak0Melih Okcu1Kamil Haliloğlu2Aras Türkoğlu3Alireza Pour-Aboughadareh4Bita Jamshidi5Tibor Janda6Azize Alaylı7Hayrunnisa Nadaroğlu8Department of Field Crops, Faculty of Agriculture, Ataturk University, 25240 Erzurum, TürkiyeDepartment of Field Crops, Faculty of Agriculture, Ataturk University, 25240 Erzurum, TürkiyeDepartment of Field Crops, Faculty of Agriculture, Ataturk University, 25240 Erzurum, TürkiyeDepartment of Field Crops, Faculty of Agriculture, Necmettin Erbakan University, 42310 Konya, TürkiyeSeed and Plant Improvement Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj P.O. Box 3158854119, IranDepartment of Food Security and Public Health, Khabat Technical Institute, Erbil Polytechnic University, Erbil 44001, IraqDepartment of Plant Physiology and Metabolomics, Agricultural Institute, Centre for Agricultural Research, 2462 Martonvásár, HungaryDepartment of Nursing, Faculty of Health Sciences, Sakarya University of Applied Sciences, 54187 Sakarya, TürkiyeDepartment of Food Technology, Vocational College of Technical Sciences, Ataturk University, 25240 Erzurum, TürkiyeNanotechnology is a rapidly growing field of science and technology that deals with the development of new solutions by understanding and controlling matter at the nanoscale. Since the last decade, magnesium oxide nanoparticles (MgO-NPs) have gained tremendous attention because of their unique characteristics and diverse applications in materials sciences and because they are non-toxic and relatively cheaply available materials. MgO-NPs can improve plant growth and contribute to plant tolerance of heavy metal toxicity. The effects of MgO-NPs on cowpea (<i>Vigna unguiculata</i> L. Walp.) plants were surveyed under in vitro conditions to find the optimum combination for cowpea tissue culture. The MgO-NPs used in the study were synthesized using walnut shell extract by the green synthesis method. MgO nanoparticles with 35–40 nm size was used in this research. When the size distribution of the MgO-NPs’ structure was examined, two peaks with 37.8 nm and 78.8 nm dimensions were obtained. The zeta potential of MgO-NPs dispersed in water was measured around −13.3 mV on average. The results showed that different doses of MgO-NPs applied to cowpea plant on all in vitro parameters significantly affected all measured parameters of cowpea plantlets under in vitro condition in a positive way. The best results in morphogenesis were MS medium supplemented with high MgO-NP applications (555 mg/L), resulting in a 25% increase in callus formation. The addition of Mg-NPs in the induction medium at concentrations at 370 mg/L increased shoot multiplication. The highest root length with 1.575 cm was obtained in MS medium containing 370 mg/L MgO. This study found that MgO-NPs greatly influenced the plantlets’ growth parameters and other measured traits; in addition, our results indicate that the efficiency of tissue culture of cowpea could be improved by increased application of MgO in the form of nanoparticles. In conclusion, the present work highlights the possibility of using MgO-NPs in cowpea tissue culture.https://www.mdpi.com/2073-4395/13/6/1646MgO-NPsnano fertilizercowpea feedregeneration |
| spellingShingle | Rabia Koçak Melih Okcu Kamil Haliloğlu Aras Türkoğlu Alireza Pour-Aboughadareh Bita Jamshidi Tibor Janda Azize Alaylı Hayrunnisa Nadaroğlu Magnesium Oxide Nanoparticles: An Influential Element in Cowpea (<i>Vigna unguiculata</i> L. Walp.) Tissue Culture Agronomy MgO-NPs nano fertilizer cowpea feed regeneration |
| title | Magnesium Oxide Nanoparticles: An Influential Element in Cowpea (<i>Vigna unguiculata</i> L. Walp.) Tissue Culture |
| title_full | Magnesium Oxide Nanoparticles: An Influential Element in Cowpea (<i>Vigna unguiculata</i> L. Walp.) Tissue Culture |
| title_fullStr | Magnesium Oxide Nanoparticles: An Influential Element in Cowpea (<i>Vigna unguiculata</i> L. Walp.) Tissue Culture |
| title_full_unstemmed | Magnesium Oxide Nanoparticles: An Influential Element in Cowpea (<i>Vigna unguiculata</i> L. Walp.) Tissue Culture |
| title_short | Magnesium Oxide Nanoparticles: An Influential Element in Cowpea (<i>Vigna unguiculata</i> L. Walp.) Tissue Culture |
| title_sort | magnesium oxide nanoparticles an influential element in cowpea i vigna unguiculata i l walp tissue culture |
| topic | MgO-NPs nano fertilizer cowpea feed regeneration |
| url | https://www.mdpi.com/2073-4395/13/6/1646 |
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