Bioactivity and Metabolomic Profile of Extracts Derived from Mycelial Solid Cultures of <i>Hypsizygus marmoreus</i>
The beech mushroom (<i>Hypsizygus marmoreus</i>) is a highly nutritious, edible medicinal mushroom native to East Asia. The present research investigated the impact of different substrates on the metabolite compositions of <i>H. marmoreus</i> mycelia cultivated in vitro. The...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-10-01
|
| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/11/10/2552 |
| _version_ | 1797572820032552960 |
|---|---|
| author | Paola Angelini Giancarlo Angeles Flores Gaia Cusumano Roberto Venanzoni Roberto Maria Pellegrino Gokhan Zengin Simonetta Cristina Di Simone Luigi Menghini Claudio Ferrante |
| author_facet | Paola Angelini Giancarlo Angeles Flores Gaia Cusumano Roberto Venanzoni Roberto Maria Pellegrino Gokhan Zengin Simonetta Cristina Di Simone Luigi Menghini Claudio Ferrante |
| author_sort | Paola Angelini |
| collection | DOAJ |
| description | The beech mushroom (<i>Hypsizygus marmoreus</i>) is a highly nutritious, edible medicinal mushroom native to East Asia. The present research investigated the impact of different substrates on the metabolite compositions of <i>H. marmoreus</i> mycelia cultivated in vitro. The substrates tested included malt extract agar, malt extract agar enriched with barley malt, and malt extract agar enriched with grape pomace. The study also assessed antimicrobial and antiradical activities of the extracts against gram-positive bacteria (<i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>), gram-negative bacteria (<i>Escherichia coli</i>, <i>Salmonella typhi</i>, and <i>Pseudomonas aeruginosa</i>), yeasts (<i>Candida albicans</i>, <i>C. tropicalis</i>, and <i>C. parapsilosis</i>), and dermatophytes (<i>Trichophyton mentagrophytes</i>, <i>T. tonsurans</i>, <i>T. rubrum</i>, <i>Arthroderma quadrifidum</i>, <i>A. gypseum</i>, <i>A. curreyi</i>, and <i>A. insingulare</i>). The results revealed that the <i>H. marmoreus</i> mycelia extracts demonstrated antibacterial and antifungal activities against the tested microorganisms. Extracts obtained from the cultivation in substrates enriched with either barley malt or grape pomace exhibited the highest antibacterial activity among all the tested bacterial strains except for <i>P. aeruginosa</i>. The same extracts showed the highest inhibitory effect against <i>C. albicans</i> and <i>C. parapsilosis</i>. Noteworthy, the extract from the mushroom cultivated in the substrate enriched with grape pomace also exhibited remarkable efficacy against <i>T. mentagrophytes</i> and <i>T. tonsurans.</i> Terpenoid and carbapenem compounds could be related to the antimicrobial properties of the extracts from mushrooms cultivated in substrates enriched with grape pomace. In comparison, the higher antiradical properties could be related to the content of indole compounds. In conclusion, growth substrate selection affects the nutritional and medicinal properties of <i>H. marmoreus</i>, making it a valuable contribution to the understanding of the cultivation of this mushroom. |
| first_indexed | 2024-03-10T21:01:20Z |
| format | Article |
| id | doaj.art-20aae846be6d4945aa4dd298d6e2b0cc |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T21:01:20Z |
| publishDate | 2023-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-20aae846be6d4945aa4dd298d6e2b0cc2023-11-19T17:28:30ZengMDPI AGMicroorganisms2076-26072023-10-011110255210.3390/microorganisms11102552Bioactivity and Metabolomic Profile of Extracts Derived from Mycelial Solid Cultures of <i>Hypsizygus marmoreus</i>Paola Angelini0Giancarlo Angeles Flores1Gaia Cusumano2Roberto Venanzoni3Roberto Maria Pellegrino4Gokhan Zengin5Simonetta Cristina Di Simone6Luigi Menghini7Claudio Ferrante8Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, ItalyDepartment of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, ItalyPhysiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, 42130 Konya, TurkeyBotanic Garden “Giardino dei Semplici”, Department of Pharmacy, “Gabriele d’Annunzio” University, Via dei Vestini 31, 66100 Chieti, ItalyBotanic Garden “Giardino dei Semplici”, Department of Pharmacy, “Gabriele d’Annunzio” University, Via dei Vestini 31, 66100 Chieti, ItalyBotanic Garden “Giardino dei Semplici”, Department of Pharmacy, “Gabriele d’Annunzio” University, Via dei Vestini 31, 66100 Chieti, ItalyThe beech mushroom (<i>Hypsizygus marmoreus</i>) is a highly nutritious, edible medicinal mushroom native to East Asia. The present research investigated the impact of different substrates on the metabolite compositions of <i>H. marmoreus</i> mycelia cultivated in vitro. The substrates tested included malt extract agar, malt extract agar enriched with barley malt, and malt extract agar enriched with grape pomace. The study also assessed antimicrobial and antiradical activities of the extracts against gram-positive bacteria (<i>Bacillus subtilis</i> and <i>Staphylococcus aureus</i>), gram-negative bacteria (<i>Escherichia coli</i>, <i>Salmonella typhi</i>, and <i>Pseudomonas aeruginosa</i>), yeasts (<i>Candida albicans</i>, <i>C. tropicalis</i>, and <i>C. parapsilosis</i>), and dermatophytes (<i>Trichophyton mentagrophytes</i>, <i>T. tonsurans</i>, <i>T. rubrum</i>, <i>Arthroderma quadrifidum</i>, <i>A. gypseum</i>, <i>A. curreyi</i>, and <i>A. insingulare</i>). The results revealed that the <i>H. marmoreus</i> mycelia extracts demonstrated antibacterial and antifungal activities against the tested microorganisms. Extracts obtained from the cultivation in substrates enriched with either barley malt or grape pomace exhibited the highest antibacterial activity among all the tested bacterial strains except for <i>P. aeruginosa</i>. The same extracts showed the highest inhibitory effect against <i>C. albicans</i> and <i>C. parapsilosis</i>. Noteworthy, the extract from the mushroom cultivated in the substrate enriched with grape pomace also exhibited remarkable efficacy against <i>T. mentagrophytes</i> and <i>T. tonsurans.</i> Terpenoid and carbapenem compounds could be related to the antimicrobial properties of the extracts from mushrooms cultivated in substrates enriched with grape pomace. In comparison, the higher antiradical properties could be related to the content of indole compounds. In conclusion, growth substrate selection affects the nutritional and medicinal properties of <i>H. marmoreus</i>, making it a valuable contribution to the understanding of the cultivation of this mushroom.https://www.mdpi.com/2076-2607/11/10/2552<i>Hypsizygus marmoreus</i>medicinal mushroomantimicrobial activityantioxidant activitymetabolomics |
| spellingShingle | Paola Angelini Giancarlo Angeles Flores Gaia Cusumano Roberto Venanzoni Roberto Maria Pellegrino Gokhan Zengin Simonetta Cristina Di Simone Luigi Menghini Claudio Ferrante Bioactivity and Metabolomic Profile of Extracts Derived from Mycelial Solid Cultures of <i>Hypsizygus marmoreus</i> Microorganisms <i>Hypsizygus marmoreus</i> medicinal mushroom antimicrobial activity antioxidant activity metabolomics |
| title | Bioactivity and Metabolomic Profile of Extracts Derived from Mycelial Solid Cultures of <i>Hypsizygus marmoreus</i> |
| title_full | Bioactivity and Metabolomic Profile of Extracts Derived from Mycelial Solid Cultures of <i>Hypsizygus marmoreus</i> |
| title_fullStr | Bioactivity and Metabolomic Profile of Extracts Derived from Mycelial Solid Cultures of <i>Hypsizygus marmoreus</i> |
| title_full_unstemmed | Bioactivity and Metabolomic Profile of Extracts Derived from Mycelial Solid Cultures of <i>Hypsizygus marmoreus</i> |
| title_short | Bioactivity and Metabolomic Profile of Extracts Derived from Mycelial Solid Cultures of <i>Hypsizygus marmoreus</i> |
| title_sort | bioactivity and metabolomic profile of extracts derived from mycelial solid cultures of i hypsizygus marmoreus i |
| topic | <i>Hypsizygus marmoreus</i> medicinal mushroom antimicrobial activity antioxidant activity metabolomics |
| url | https://www.mdpi.com/2076-2607/11/10/2552 |
| work_keys_str_mv | AT paolaangelini bioactivityandmetabolomicprofileofextractsderivedfrommycelialsolidculturesofihypsizygusmarmoreusi AT giancarloangelesflores bioactivityandmetabolomicprofileofextractsderivedfrommycelialsolidculturesofihypsizygusmarmoreusi AT gaiacusumano bioactivityandmetabolomicprofileofextractsderivedfrommycelialsolidculturesofihypsizygusmarmoreusi AT robertovenanzoni bioactivityandmetabolomicprofileofextractsderivedfrommycelialsolidculturesofihypsizygusmarmoreusi AT robertomariapellegrino bioactivityandmetabolomicprofileofextractsderivedfrommycelialsolidculturesofihypsizygusmarmoreusi AT gokhanzengin bioactivityandmetabolomicprofileofextractsderivedfrommycelialsolidculturesofihypsizygusmarmoreusi AT simonettacristinadisimone bioactivityandmetabolomicprofileofextractsderivedfrommycelialsolidculturesofihypsizygusmarmoreusi AT luigimenghini bioactivityandmetabolomicprofileofextractsderivedfrommycelialsolidculturesofihypsizygusmarmoreusi AT claudioferrante bioactivityandmetabolomicprofileofextractsderivedfrommycelialsolidculturesofihypsizygusmarmoreusi |