Towards optimising the production of and expression from polycistronic vectors in embryonic stem cells.

Towards optimising the production of and expression from polycistronic vectors in embryonic stem cells.

Polycistronic vectors linked by self-processing 2A peptides have been successfully used in cellular reprogramming. The expression of these vectors has yet to be well documented in embryonic stem cells. In the present study, we generated expression cassettes containing combinatorial arrangements of 3...

Full description

Bibliographic Details
Main Authors:	Steven Y Gao, Michelle M Jack, Christopher O'Neill
Format:	Article
Language:	English
Published:	Public Library of Science (PLoS) 2012-01-01
Series:	PLoS ONE
Online Access:	http://europepmc.org/articles/PMC3490874?pdf=render

Similar Items

Production of lentiviral vector with polycistronic transcripts for reprogramming of mouse fibroblast cells
by: Al Abbar, Akram, et al.
Published: (2018)

Polycistronic gene expression in Aspergillus niger
by: Tabea Schuetze, et al.
Published: (2017-09-01)

Establishing Polycistronic Expression in the Model Microorganism Ustilago maydis
by: Kira Müntjes, et al.
Published: (2020-06-01)

Generation of induced pluripotent stem cells by a polycistronic lentiviral vector in feeder- and serum- free defined culture
by: Al Abbar, Akram Faisal Mahmaud, et al.
Published: (2018)

Generation of mouse induced pluripotent stem cells using polycistronic lentiviral vector in feeder - and serum-free culture
by: Mahmaud, Akram Faisal
Published: (2015)

Synthetic polycistronic sequences in eukaryotes
by: Xuekun Wang, et al.
Published: (2021-12-01)

Direct conversion of human fibroblast to hepatocytes using a single inducible polycistronic vector
by: Maria Ballester, et al.
Published: (2019-11-01)

Systematic comparison of 2A peptides for cloning multi-genes in a polycistronic vector
by: Ziqing Liu, et al.
Published: (2017-05-01)

A polycistronic system for multiplexed and precalibrated expression of multigene pathways in fungi
by: Qun Yue, et al.
Published: (2023-07-01)

Polycistronic Expression System for Pichia pastoris Composed of Chitino- and Chitosanolytic Enzymes
by: Michal B. Kaczmarek, et al.
Published: (2021-08-01)

Expression Regulation of Polycistronic lee3 Genes of Enterohaemorrhagic Escherichia coli.
by: Wei-Sheng W Sun, et al.
Published: (2016-01-01)

Golden Gate Cloning-Compatible DNA Replicon/2A-Mediated Polycistronic Vectors for Plants
by: Jae Hoon Lee, et al.
Published: (2020-10-01)

Efficient conversion of astrocytes to functional midbrain dopaminergic neurons using a single polycistronic vector.
by: Russell C Addis, et al.
Published: (2011-01-01)

Generation of hemophilia A mouse induced pluripotent stem cells using polycistronic lentiviral vector in serum- and feeder-free culture conditions
by: M. Sakri, Rozita, et al.
Published: (2016)

The epigenetic bivalency of core pancreatic β-cell transcription factor genes within mouse pluripotent embryonic stem cells is not affected by knockdown of the polycomb repressive complex 2, SUZ12.
by: Jennifer C Y Wong, et al.
Published: (2014-01-01)

Polycistronic Expression of Multi-Subunit Complexes in the Eukaryotic Environment: A Narrative Review
by: Afshin Abdi Ghavidel, et al.
Published: (2022-09-01)

Development of Polycistronic Baculovirus Surface Display Vectors to Simultaneously Express Viral Proteins of Porcine Reproductive and Respiratory Syndrome and Analysis of Their Immunogenicity in Swine
by: Chao-Yu Hsu, et al.
Published: (2023-10-01)

RANGE: Gene Transfer of Reversibly Controlled Polycistronic Genes
by: Yiwei Chen, et al.
Published: (2013-01-01)

ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger
by: Elena Geib, et al.
Published: (2017-12-01)

Construction of polycistronic baculovirus surface display vectors to express the PCV2 Cap(d41) protein and analysis of its immunogenicity in mice and swine
by: Ya-Yi Chen, et al.
Published: (2020-09-01)

Efficient generation of fully reprogrammed human iPS cells via polycistronic retroviral vector and a new cocktail of chemical compounds.
by: Zhonghui Zhang, et al.
Published: (2011-01-01)

Histone acetylations mark origins of polycistronic transcription in <it>Leishmania major</it>
by: Campbell David A, et al.
Published: (2009-04-01)

Expression of Cry1Ab and Cry2Ab by a polycistronic transgene with a self-cleavage peptide in rice.
by: Qichao Zhao, et al.
Published: (2014-01-01)

Efficient inhibition of HIV-1 replication by an artificial polycistronic miRNA construct
by: Zhang Tao, et al.
Published: (2012-06-01)

sRNA expedites polycistronic mRNA decay in Escherichia coli
by: Heung Jin Jeon, et al.
Published: (2023-03-01)

XRN2 Autoregulation and Control of Polycistronic Gene Expresssion in Caenorhabditis elegans.
by: Takashi S Miki, et al.
Published: (2016-09-01)

Polar mutagenesis of polycistronic bacterial transcriptional units using Cas12a
by: Antoine Graffeuil, et al.
Published: (2022-07-01)

Widespread Polycistronic Transcripts in Fungi Revealed by Single-Molecule mRNA Sequencing.
by: Sean P Gordon, et al.
Published: (2015-01-01)

Ribosome Shunting, Polycistronic Translation, and Evasion of Antiviral Defenses in Plant Pararetroviruses and Beyond
by: Mikhail M. Pooggin, et al.
Published: (2018-04-01)

Correction: Efficient Generation of Fully Reprogrammed Human iPS Cells via Polycistronic Retroviral Vector and a New Cocktail of Chemical Compounds
Published: (2012-01-01)

A component of the mir-17-92 polycistronic oncomir promotes oncogene-dependent apoptosis
by: Virginie Olive, et al.
Published: (2013-10-01)

A polycistronic retroviral vector encoding hB7-1 (CD80) and hIL-2 for ex vivo cancer gene therapy.
by: Kuiper, M, et al.
Published: (1996)

Evaluation of Gaussia luciferase and foot-and-mouth disease virus 2A translational interrupter chimeras as polycistronic reporters for transgene expression
by: Michael Puckette, et al.
Published: (2017-06-01)

Vectors/
by: O'Neill, P. J.
Published: (1975)

Phylogenetic analysis and molecular evolution patterns in the MIR482-MIR1448 polycistron of Populus L.
by: Jia-Ping Zhao, et al.
Published: (2012-01-01)

RedEfish: Generation of the Polycistronic mScarlet: GSG-T2A: Ttpa Zebrafish Line
by: Brian Head, et al.
Published: (2021-06-01)

Polycistronic Artificial microRNA-Mediated Resistance to Cucumber Green Mottle Mosaic Virus in Cucumber
by: Shuo Miao, et al.
Published: (2021-11-01)

A polycistronic transgene design for combinatorial genetic perturbations from a single transcript in Drosophila.
by: Alexander G Teague, et al.
Published: (2023-06-01)

Efficient Genetic Modification of Cynomolgus Monkey Embryonic Stem Cells with Lentiviral Vectors
by: Weiqiang Li, et al.
Published: (2010-09-01)

Production of embryonic and fetal-like red blood cells from human induced pluripotent stem cells.
by: Chan-Jung Chang, et al.
Published: (2011-01-01)