Effect of <i>Achillea fragrantissima</i> Extract on Excision Wound Biofilms of MRSA and <i>Pseudomonas aeruginosa</i> in Diabetic Mice
<i>Achillea fragrantissima</i>, a desert plant commonly known as yarrow, is traditionally used as an antimicrobial agent in folklore medicine in Saudi Arabia. The current study was undertaken to determine its antibiofilm activity against methicillin-resistant <i>Staphylococcus aure...
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MDPI AG
2023-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/11/9774 |
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| author | Yasir Almuhanna Mohammed Hussein Alqasmi Hamood AlSudais Mohammed Alrouji Fahd A. Kuriri Mohammed Alissa Meshari A. Alsuwat Mohammed Asad Babu Joseph |
| author_facet | Yasir Almuhanna Mohammed Hussein Alqasmi Hamood AlSudais Mohammed Alrouji Fahd A. Kuriri Mohammed Alissa Meshari A. Alsuwat Mohammed Asad Babu Joseph |
| author_sort | Yasir Almuhanna |
| collection | DOAJ |
| description | <i>Achillea fragrantissima</i>, a desert plant commonly known as yarrow, is traditionally used as an antimicrobial agent in folklore medicine in Saudi Arabia. The current study was undertaken to determine its antibiofilm activity against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and multi-drug-resistant <i>Pseudomonas aeruginosa</i> (MDR-<i>P. aeruginosa</i>) using in vitro and in vivo studies. A biofilm model induced through an excision wound in diabetic mice was used to evaluate its effect in vivo. The skin irritation and cytotoxic effects of the extract were determined using mice and HaCaT cell lines, respectively. The <i>Achillea fragrantissima</i> methanolic extract was analyzed with LC-MS to detect different phytoconstituents, which revealed the presence of 47 different phytoconstituents. The extract inhibited the growth of both tested pathogens in vitro. It also increased the healing of biofilm-formed excision wounds, demonstrating its antibiofilm, antimicrobial, and wound-healing action in vivo. The effect of the extract was concentration-dependent, and its activity was stronger against MRSA than MDR-<i>P. aeruginosa</i>. The extract formulation was devoid of a skin irritation effect in vivo and cytotoxic effect on HaCaT cell lines in vitro. |
| first_indexed | 2024-03-11T03:04:22Z |
| format | Article |
| id | doaj.art-20ac73a771044efeaa0b2f04cdec6a3d |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T03:04:22Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-20ac73a771044efeaa0b2f04cdec6a3d2023-11-18T08:03:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-06-012411977410.3390/ijms24119774Effect of <i>Achillea fragrantissima</i> Extract on Excision Wound Biofilms of MRSA and <i>Pseudomonas aeruginosa</i> in Diabetic MiceYasir Almuhanna0Mohammed Hussein Alqasmi1Hamood AlSudais2Mohammed Alrouji3Fahd A. Kuriri4Mohammed Alissa5Meshari A. Alsuwat6Mohammed Asad7Babu Joseph8Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi ArabiaChair of Medical and Molecular Genetics Research, Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, Riyadh 12372, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi ArabiaDepartment of Medical Laboratory Sciences, College of Applied Medical Sciences, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi ArabiaClinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, Al-Taif 21974, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi ArabiaDepartment of Clinical Laboratory Sciences, College of Applied Medical Sciences, Shaqra University, Shaqra 11961, Saudi Arabia<i>Achillea fragrantissima</i>, a desert plant commonly known as yarrow, is traditionally used as an antimicrobial agent in folklore medicine in Saudi Arabia. The current study was undertaken to determine its antibiofilm activity against methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) and multi-drug-resistant <i>Pseudomonas aeruginosa</i> (MDR-<i>P. aeruginosa</i>) using in vitro and in vivo studies. A biofilm model induced through an excision wound in diabetic mice was used to evaluate its effect in vivo. The skin irritation and cytotoxic effects of the extract were determined using mice and HaCaT cell lines, respectively. The <i>Achillea fragrantissima</i> methanolic extract was analyzed with LC-MS to detect different phytoconstituents, which revealed the presence of 47 different phytoconstituents. The extract inhibited the growth of both tested pathogens in vitro. It also increased the healing of biofilm-formed excision wounds, demonstrating its antibiofilm, antimicrobial, and wound-healing action in vivo. The effect of the extract was concentration-dependent, and its activity was stronger against MRSA than MDR-<i>P. aeruginosa</i>. The extract formulation was devoid of a skin irritation effect in vivo and cytotoxic effect on HaCaT cell lines in vitro.https://www.mdpi.com/1422-0067/24/11/9774HaCaT cell linesdiabeticcytotoxicityskin irritationLC-MS |
| spellingShingle | Yasir Almuhanna Mohammed Hussein Alqasmi Hamood AlSudais Mohammed Alrouji Fahd A. Kuriri Mohammed Alissa Meshari A. Alsuwat Mohammed Asad Babu Joseph Effect of <i>Achillea fragrantissima</i> Extract on Excision Wound Biofilms of MRSA and <i>Pseudomonas aeruginosa</i> in Diabetic Mice International Journal of Molecular Sciences HaCaT cell lines diabetic cytotoxicity skin irritation LC-MS |
| title | Effect of <i>Achillea fragrantissima</i> Extract on Excision Wound Biofilms of MRSA and <i>Pseudomonas aeruginosa</i> in Diabetic Mice |
| title_full | Effect of <i>Achillea fragrantissima</i> Extract on Excision Wound Biofilms of MRSA and <i>Pseudomonas aeruginosa</i> in Diabetic Mice |
| title_fullStr | Effect of <i>Achillea fragrantissima</i> Extract on Excision Wound Biofilms of MRSA and <i>Pseudomonas aeruginosa</i> in Diabetic Mice |
| title_full_unstemmed | Effect of <i>Achillea fragrantissima</i> Extract on Excision Wound Biofilms of MRSA and <i>Pseudomonas aeruginosa</i> in Diabetic Mice |
| title_short | Effect of <i>Achillea fragrantissima</i> Extract on Excision Wound Biofilms of MRSA and <i>Pseudomonas aeruginosa</i> in Diabetic Mice |
| title_sort | effect of i achillea fragrantissima i extract on excision wound biofilms of mrsa and i pseudomonas aeruginosa i in diabetic mice |
| topic | HaCaT cell lines diabetic cytotoxicity skin irritation LC-MS |
| url | https://www.mdpi.com/1422-0067/24/11/9774 |
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