Differential microRNA expression profile of Portunus trituberculatus under florfenicol stress
Florfenicol (FLR), a broad-spectrum antibacterial agent, is commonly used in the breeding and cultivation of aquatic animals. microRNAs (miRNAs) control gene expression by mediating post-transcriptional mRNA repression. However, not much has been reported regarding their functions when crustaceans e...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-04-01
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| Series: | Aquaculture Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423000145 |
| _version_ | 1827981796114956288 |
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| author | Yao Xu Xianyun Ren Yuefeng Cai Jianjian Lv Xianliang Meng Baoquan Gao Ping Liu Jian Li |
| author_facet | Yao Xu Xianyun Ren Yuefeng Cai Jianjian Lv Xianliang Meng Baoquan Gao Ping Liu Jian Li |
| author_sort | Yao Xu |
| collection | DOAJ |
| description | Florfenicol (FLR), a broad-spectrum antibacterial agent, is commonly used in the breeding and cultivation of aquatic animals. microRNAs (miRNAs) control gene expression by mediating post-transcriptional mRNA repression. However, not much has been reported regarding their functions when crustaceans encounter FLR stress. Herein we observed histological alterations and performed small RNA sequencing to characterize differentially expressed miRNAs in the hepatopancreas of Portunus trituberculatus exposed to FLR (80 mg/kg) stress for 3 h and 12 h. Our results indicated irregularities in the tubular structure of hepatopancreas, separation between epithelium and myoepithelial layer, ruptured epithelial cells, and tubule degeneration. Thirty-five (19 up- and 16 downregulated) and 31 (17 up- and 14 downregulated) differentially expressed miRNAs were detected between Group-C and Group-FLR-3 h and between Group-C and Group-FLR-12 h, respectively. Dual luciferase reporter assay was performed for predicting target genes of miR-34 and miR-263b. We found that mir-263b directly inhibited cytochrome C expression at the cell level in vitro. Functional analyses of predicted target genes of differentially expressed miRNAs revealed that FLR stress induced xenobiotic metabolism, immune toxicity, and cell apoptosis, as well as influenced diverse metabolic processes. To our knowledge, this is the first report on the response of P. trituberculatus to FLR stress. Our findings provide evidence that miRNAs participate in cell apoptosis and immune regulation in crabs. |
| first_indexed | 2024-04-09T22:14:45Z |
| format | Article |
| id | doaj.art-20ad045e45824e0bb5b810b2846b568f |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-04-09T22:14:45Z |
| publishDate | 2023-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-20ad045e45824e0bb5b810b2846b568f2023-03-23T04:36:15ZengElsevierAquaculture Reports2352-51342023-04-0129101475Differential microRNA expression profile of Portunus trituberculatus under florfenicol stressYao Xu0Xianyun Ren1Yuefeng Cai2Jianjian Lv3Xianliang Meng4Baoquan Gao5Ping Liu6Jian Li7Key Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China; Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, PR ChinaKey Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR China; Correspondence to: Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, 106 Nanjing Road, Qingdao 266003, PR China.Jiangsu Key Laboratory of Marine Bioresources and Environment/Jiangsu Key Laboratory of Marine Biotechnology, Huaihai Institute of Technology, Lianyungang, PR ChinaKey Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR ChinaKey Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR ChinaKey Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR ChinaKey Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR ChinaKey Laboratory for Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, PR China; Function Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, PR ChinaFlorfenicol (FLR), a broad-spectrum antibacterial agent, is commonly used in the breeding and cultivation of aquatic animals. microRNAs (miRNAs) control gene expression by mediating post-transcriptional mRNA repression. However, not much has been reported regarding their functions when crustaceans encounter FLR stress. Herein we observed histological alterations and performed small RNA sequencing to characterize differentially expressed miRNAs in the hepatopancreas of Portunus trituberculatus exposed to FLR (80 mg/kg) stress for 3 h and 12 h. Our results indicated irregularities in the tubular structure of hepatopancreas, separation between epithelium and myoepithelial layer, ruptured epithelial cells, and tubule degeneration. Thirty-five (19 up- and 16 downregulated) and 31 (17 up- and 14 downregulated) differentially expressed miRNAs were detected between Group-C and Group-FLR-3 h and between Group-C and Group-FLR-12 h, respectively. Dual luciferase reporter assay was performed for predicting target genes of miR-34 and miR-263b. We found that mir-263b directly inhibited cytochrome C expression at the cell level in vitro. Functional analyses of predicted target genes of differentially expressed miRNAs revealed that FLR stress induced xenobiotic metabolism, immune toxicity, and cell apoptosis, as well as influenced diverse metabolic processes. To our knowledge, this is the first report on the response of P. trituberculatus to FLR stress. Our findings provide evidence that miRNAs participate in cell apoptosis and immune regulation in crabs.http://www.sciencedirect.com/science/article/pii/S2352513423000145Portunus trituberculatusFlorfenicolMiRNA-seqDual luciferase reporter assayImmunityApoptosis |
| spellingShingle | Yao Xu Xianyun Ren Yuefeng Cai Jianjian Lv Xianliang Meng Baoquan Gao Ping Liu Jian Li Differential microRNA expression profile of Portunus trituberculatus under florfenicol stress Aquaculture Reports Portunus trituberculatus Florfenicol MiRNA-seq Dual luciferase reporter assay Immunity Apoptosis |
| title | Differential microRNA expression profile of Portunus trituberculatus under florfenicol stress |
| title_full | Differential microRNA expression profile of Portunus trituberculatus under florfenicol stress |
| title_fullStr | Differential microRNA expression profile of Portunus trituberculatus under florfenicol stress |
| title_full_unstemmed | Differential microRNA expression profile of Portunus trituberculatus under florfenicol stress |
| title_short | Differential microRNA expression profile of Portunus trituberculatus under florfenicol stress |
| title_sort | differential microrna expression profile of portunus trituberculatus under florfenicol stress |
| topic | Portunus trituberculatus Florfenicol MiRNA-seq Dual luciferase reporter assay Immunity Apoptosis |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423000145 |
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