Utility of P16/INK4a and Ki-67 in Preneoplasticand Neoplastic Lesions of Cervix
Background and Objective: The currentstudy aimed at investigating the histomorphological spectrum of cervical intraepithelial and invasive lesions assessing the diagnostic significance of P16/INK4a and Ki-67 in such lesions, andcorrelatingP16/INK4a and Ki-67 immunoexpression with histologic type and...
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| Format: | Article |
| Language: | English |
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Iranian Society of Pathology
2018-07-01
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| Series: | Iranian Journal of Pathology |
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| Online Access: | https://ijp.iranpath.org/article_31844_a200e760e4f16ac5aa5419d1e9a92364.pdf |
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| author | Ashok Sangwaiya Meenu Gill Shilpa Bairwa Manish Chaudhry Rajeev Sen Sant Prakash Kataria |
| author_facet | Ashok Sangwaiya Meenu Gill Shilpa Bairwa Manish Chaudhry Rajeev Sen Sant Prakash Kataria |
| author_sort | Ashok Sangwaiya |
| collection | DOAJ |
| description | Background and Objective: The currentstudy aimed at investigating the histomorphological spectrum of cervical intraepithelial and invasive lesions assessing the diagnostic significance of P16/INK4a and Ki-67 in such lesions, andcorrelatingP16/INK4a and Ki-67 immunoexpression with histologic type and grade.Methods: A total of 60 cases were selectedcomprising 10 cases withchronic cervicitis, 29 cases withcervical intraepithelial neoplasia(CIN), and 21 cases withsquamous cell carcinoma. These cases were evaluated morphologically and immunohistochemically with P16 and Ki-67.Results: There was no expression of P16 and Ki-67 in 10 (100%) cases withchronic cervicitis while in CIN, it was expressed in 25 (86.20%) cases and in carcinoma it was expressed in 20 (95.23%) cases. Ki-67 was expressed in 28 (96.55%) cases withCIN and in 100% of cases withcarcinoma.Conclusion: Cervical carcinoma is a significant contributor to cancer-related morbidity and mortality worldwide. Identification of bio-markers in cervical neoplasia is necessary to distinguish CIN from other non-neoplastic cervical lesions to prevent under treatmentor overtreatment as the histomorphological features alone are not sufficient.Significant upregulation of P16, cyclin dependent kinase inhibitor, and Ki-67, a nuclear non-histone protein, was observed in carcinoma cervix and with the increasing severity of CINs. Correlation between grades of P16 and Ki-67 among cervical pre-neoplasia and neoplasia showed an increasing P16 expression with consistently increasing Ki-67 labelling index in the groups with theincreasing severity. |
| first_indexed | 2024-12-19T12:55:30Z |
| format | Article |
| id | doaj.art-20ae7ebcdda8476d8b0aca2a8fafb734 |
| institution | Directory Open Access Journal |
| issn | 1735-5303 2345-3656 |
| language | English |
| last_indexed | 2024-12-19T12:55:30Z |
| publishDate | 2018-07-01 |
| publisher | Iranian Society of Pathology |
| record_format | Article |
| series | Iranian Journal of Pathology |
| spelling | doaj.art-20ae7ebcdda8476d8b0aca2a8fafb7342022-12-21T20:20:23ZengIranian Society of PathologyIranian Journal of Pathology1735-53032345-36562018-07-0113330831631844Utility of P16/INK4a and Ki-67 in Preneoplasticand Neoplastic Lesions of CervixAshok Sangwaiya0Meenu Gill1Shilpa Bairwa2Manish Chaudhry3Rajeev Sen4Sant Prakash Kataria5Dept. of Pathology, SHKM, GMC, Nalhar, Mewat, Haryana, IndiaDept. of Pathology, Pt. B D Sharma, PGIMS, Rohtak, Haryana, IndiaDept. of Pathology, SHKM, GMC, Nalhar, Mewat, Haryana, IndiaDept. of Pathology, MM Institute of Medical Sciences and Research, Mullana, Ambala, Haryana, IndiaDept. of Pathology, Pt. B D Sharma, PGIMS, Rohtak, Haryana, IndiaDept. of Pathology, Pt. B D Sharma, PGIMS, Rohtak, Haryana, IndiaBackground and Objective: The currentstudy aimed at investigating the histomorphological spectrum of cervical intraepithelial and invasive lesions assessing the diagnostic significance of P16/INK4a and Ki-67 in such lesions, andcorrelatingP16/INK4a and Ki-67 immunoexpression with histologic type and grade.Methods: A total of 60 cases were selectedcomprising 10 cases withchronic cervicitis, 29 cases withcervical intraepithelial neoplasia(CIN), and 21 cases withsquamous cell carcinoma. These cases were evaluated morphologically and immunohistochemically with P16 and Ki-67.Results: There was no expression of P16 and Ki-67 in 10 (100%) cases withchronic cervicitis while in CIN, it was expressed in 25 (86.20%) cases and in carcinoma it was expressed in 20 (95.23%) cases. Ki-67 was expressed in 28 (96.55%) cases withCIN and in 100% of cases withcarcinoma.Conclusion: Cervical carcinoma is a significant contributor to cancer-related morbidity and mortality worldwide. Identification of bio-markers in cervical neoplasia is necessary to distinguish CIN from other non-neoplastic cervical lesions to prevent under treatmentor overtreatment as the histomorphological features alone are not sufficient.Significant upregulation of P16, cyclin dependent kinase inhibitor, and Ki-67, a nuclear non-histone protein, was observed in carcinoma cervix and with the increasing severity of CINs. Correlation between grades of P16 and Ki-67 among cervical pre-neoplasia and neoplasia showed an increasing P16 expression with consistently increasing Ki-67 labelling index in the groups with theincreasing severity.https://ijp.iranpath.org/article_31844_a200e760e4f16ac5aa5419d1e9a92364.pdfcervical carcinomacervical intraepithelial neoplasiadysplasiahuman papillomaviruski-67p16 |
| spellingShingle | Ashok Sangwaiya Meenu Gill Shilpa Bairwa Manish Chaudhry Rajeev Sen Sant Prakash Kataria Utility of P16/INK4a and Ki-67 in Preneoplasticand Neoplastic Lesions of Cervix Iranian Journal of Pathology cervical carcinoma cervical intraepithelial neoplasia dysplasia human papillomavirus ki-67 p16 |
| title | Utility of P16/INK4a and Ki-67 in Preneoplasticand Neoplastic Lesions of Cervix |
| title_full | Utility of P16/INK4a and Ki-67 in Preneoplasticand Neoplastic Lesions of Cervix |
| title_fullStr | Utility of P16/INK4a and Ki-67 in Preneoplasticand Neoplastic Lesions of Cervix |
| title_full_unstemmed | Utility of P16/INK4a and Ki-67 in Preneoplasticand Neoplastic Lesions of Cervix |
| title_short | Utility of P16/INK4a and Ki-67 in Preneoplasticand Neoplastic Lesions of Cervix |
| title_sort | utility of p16 ink4a and ki 67 in preneoplasticand neoplastic lesions of cervix |
| topic | cervical carcinoma cervical intraepithelial neoplasia dysplasia human papillomavirus ki-67 p16 |
| url | https://ijp.iranpath.org/article_31844_a200e760e4f16ac5aa5419d1e9a92364.pdf |
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