Effect of Seed Priming with Chitosan Hydrolysate on Lettuce (<i>Lactuca sativa</i>) Growth Parameters
Seed priming increases germination, yield, and resistance to abiotic factors and phytopathogens. Chitosan is considered an ecofriendly growth stimulant and crop protection agent. Chitosan hydrolysate (CH) is an unfractionated product of hydrolysis of high-molecular-weight crab shell chitosan with a...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/28/4/1915 |
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| author | Tatiana Lyalina Balzhima Shagdarova Yuliya Zhuikova Alla Il’ina Alexey Lunkov Valery Varlamov |
| author_facet | Tatiana Lyalina Balzhima Shagdarova Yuliya Zhuikova Alla Il’ina Alexey Lunkov Valery Varlamov |
| author_sort | Tatiana Lyalina |
| collection | DOAJ |
| description | Seed priming increases germination, yield, and resistance to abiotic factors and phytopathogens. Chitosan is considered an ecofriendly growth stimulant and crop protection agent. Chitosan hydrolysate (CH) is an unfractionated product of hydrolysis of high-molecular-weight crab shell chitosan with a molecular weight of 1040 kDa and a degree of deacetylation of 85% with nitric acid. The average molecular weight of the main fraction in CH was 39 kDa. Lettuce seeds were soaked in 0.01–1 mg/mL CH for 6 h before sowing. The effects of CH on seed germination, plant morphology, and biochemical indicators at different growth stages were evaluated. Under the 0.1 mg/mL CH treatment, earlier seed germination was detected compared to the control. Increased root branching was observed, along with 100% and 67% increases in fresh weight (FW) at the 24th and 38th days after sowing (DAS), respectively. An increase in the shoot FW was found in CH-treated plants (33% and 4% at the 24th and 38th DAS, respectively). Significant increases in chlorophyll and carotenoid content compared to the control were observed at the 10th DAS. There were no significant differences in the activity of phenylalanine ammonia-lyase, polyphenol oxidase, β-1,3-glucanase, and chitinase at the 24th and 38th DAS. Seed priming with CH could increase the yield and uniformity of plants within the group. This effect is important for commercial vegetable production. |
| first_indexed | 2024-03-11T08:20:46Z |
| format | Article |
| id | doaj.art-20b2da85f25148c38e9d7de3dc5ac165 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-11T08:20:46Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-20b2da85f25148c38e9d7de3dc5ac1652023-11-16T22:24:58ZengMDPI AGMolecules1420-30492023-02-01284191510.3390/molecules28041915Effect of Seed Priming with Chitosan Hydrolysate on Lettuce (<i>Lactuca sativa</i>) Growth ParametersTatiana Lyalina0Balzhima Shagdarova1Yuliya Zhuikova2Alla Il’ina3Alexey Lunkov4Valery Varlamov5Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., 119071 Moscow, RussiaInstitute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, 33, bld. 2 Leninsky Ave., 119071 Moscow, RussiaSeed priming increases germination, yield, and resistance to abiotic factors and phytopathogens. Chitosan is considered an ecofriendly growth stimulant and crop protection agent. Chitosan hydrolysate (CH) is an unfractionated product of hydrolysis of high-molecular-weight crab shell chitosan with a molecular weight of 1040 kDa and a degree of deacetylation of 85% with nitric acid. The average molecular weight of the main fraction in CH was 39 kDa. Lettuce seeds were soaked in 0.01–1 mg/mL CH for 6 h before sowing. The effects of CH on seed germination, plant morphology, and biochemical indicators at different growth stages were evaluated. Under the 0.1 mg/mL CH treatment, earlier seed germination was detected compared to the control. Increased root branching was observed, along with 100% and 67% increases in fresh weight (FW) at the 24th and 38th days after sowing (DAS), respectively. An increase in the shoot FW was found in CH-treated plants (33% and 4% at the 24th and 38th DAS, respectively). Significant increases in chlorophyll and carotenoid content compared to the control were observed at the 10th DAS. There were no significant differences in the activity of phenylalanine ammonia-lyase, polyphenol oxidase, β-1,3-glucanase, and chitinase at the 24th and 38th DAS. Seed priming with CH could increase the yield and uniformity of plants within the group. This effect is important for commercial vegetable production.https://www.mdpi.com/1420-3049/28/4/1915chitosanchitosan hydrolysatepolysaccharidegrowth stimulantseed priminggermination |
| spellingShingle | Tatiana Lyalina Balzhima Shagdarova Yuliya Zhuikova Alla Il’ina Alexey Lunkov Valery Varlamov Effect of Seed Priming with Chitosan Hydrolysate on Lettuce (<i>Lactuca sativa</i>) Growth Parameters Molecules chitosan chitosan hydrolysate polysaccharide growth stimulant seed priming germination |
| title | Effect of Seed Priming with Chitosan Hydrolysate on Lettuce (<i>Lactuca sativa</i>) Growth Parameters |
| title_full | Effect of Seed Priming with Chitosan Hydrolysate on Lettuce (<i>Lactuca sativa</i>) Growth Parameters |
| title_fullStr | Effect of Seed Priming with Chitosan Hydrolysate on Lettuce (<i>Lactuca sativa</i>) Growth Parameters |
| title_full_unstemmed | Effect of Seed Priming with Chitosan Hydrolysate on Lettuce (<i>Lactuca sativa</i>) Growth Parameters |
| title_short | Effect of Seed Priming with Chitosan Hydrolysate on Lettuce (<i>Lactuca sativa</i>) Growth Parameters |
| title_sort | effect of seed priming with chitosan hydrolysate on lettuce i lactuca sativa i growth parameters |
| topic | chitosan chitosan hydrolysate polysaccharide growth stimulant seed priming germination |
| url | https://www.mdpi.com/1420-3049/28/4/1915 |
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