Identification of novel spp. of rice and wheat endophytic diazotrophs by 16S rDNA gene and FTIR analysis
In this research, six isolates, including three from three rice roots (PxR1, PxR2 and StR1) and three from three wheat roots (PxW1, PxW2 and PxW3) were isolated as endophytic bacteria and except for StR1, all the isolates were identified as Pseudoxanthomonas based on phenotypic analysis including FT...
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| Format: | Article |
| Language: | fas |
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University of Isfahan
2012-06-01
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| Series: | تاکسونومی و بیوسیستماتیک |
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| Online Access: | http://uijs.ui.ac.ir/tbj/browse.php?a_code=A-10-66-2&slc_lang=en&sid=1 |
| _version_ | 1797758896253698048 |
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| author | Mohammad Javad Mehdipour Moghaddam Giti Emtiazi Majid Bouzari Zivar Salehi |
| author_facet | Mohammad Javad Mehdipour Moghaddam Giti Emtiazi Majid Bouzari Zivar Salehi |
| author_sort | Mohammad Javad Mehdipour Moghaddam |
| collection | DOAJ |
| description | In this research, six isolates, including three from three rice roots (PxR1, PxR2 and StR1) and three from three wheat roots (PxW1, PxW2 and PxW3) were isolated as endophytic bacteria and except for StR1, all the isolates were identified as Pseudoxanthomonas based on phenotypic analysis including FTIR and PCR amplification of 16S rDNA. The results showed that PxR1, PxR2, PxW1 and PxW2 were all similar and belonged to a novel species of Pseudoxanthomonas, but PxW3 was from different species. StR1 belonged to a novel species of Stenotrophomonas. Two strains including Azospirillum brasiliense Sp7 (S1) and Azospirillum lipoferum (S2) were selected as standard strains and compared with those isolates however, phenotypic and genotypic analysis verified that those isolates were not Azospirillum. For the first time, it was indicated that Pseudoxanthomonas existed as an endophytic bacterium in rice root. |
| first_indexed | 2024-03-12T18:36:37Z |
| format | Article |
| id | doaj.art-20b573146794484db8bfcf981702d4da |
| institution | Directory Open Access Journal |
| issn | 2008-8906 2322-2190 |
| language | fas |
| last_indexed | 2024-03-12T18:36:37Z |
| publishDate | 2012-06-01 |
| publisher | University of Isfahan |
| record_format | Article |
| series | تاکسونومی و بیوسیستماتیک |
| spelling | doaj.art-20b573146794484db8bfcf981702d4da2023-08-02T07:58:38ZfasUniversity of Isfahanتاکسونومی و بیوسیستماتیک2008-89062322-21902012-06-01410110Identification of novel spp. of rice and wheat endophytic diazotrophs by 16S rDNA gene and FTIR analysisMohammad Javad Mehdipour Moghaddam0Giti Emtiazi1Majid Bouzari2Zivar Salehi3 In this research, six isolates, including three from three rice roots (PxR1, PxR2 and StR1) and three from three wheat roots (PxW1, PxW2 and PxW3) were isolated as endophytic bacteria and except for StR1, all the isolates were identified as Pseudoxanthomonas based on phenotypic analysis including FTIR and PCR amplification of 16S rDNA. The results showed that PxR1, PxR2, PxW1 and PxW2 were all similar and belonged to a novel species of Pseudoxanthomonas, but PxW3 was from different species. StR1 belonged to a novel species of Stenotrophomonas. Two strains including Azospirillum brasiliense Sp7 (S1) and Azospirillum lipoferum (S2) were selected as standard strains and compared with those isolates however, phenotypic and genotypic analysis verified that those isolates were not Azospirillum. For the first time, it was indicated that Pseudoxanthomonas existed as an endophytic bacterium in rice root.http://uijs.ui.ac.ir/tbj/browse.php?a_code=A-10-66-2&slc_lang=en&sid=1 Endophyte Rice Wheat Azospirillum Pseudoxanthomonas Stenotrophomonas |
| spellingShingle | Mohammad Javad Mehdipour Moghaddam Giti Emtiazi Majid Bouzari Zivar Salehi Identification of novel spp. of rice and wheat endophytic diazotrophs by 16S rDNA gene and FTIR analysis تاکسونومی و بیوسیستماتیک Endophyte Rice Wheat Azospirillum Pseudoxanthomonas Stenotrophomonas |
| title | Identification of novel spp. of rice and wheat endophytic diazotrophs by 16S rDNA gene and FTIR analysis |
| title_full | Identification of novel spp. of rice and wheat endophytic diazotrophs by 16S rDNA gene and FTIR analysis |
| title_fullStr | Identification of novel spp. of rice and wheat endophytic diazotrophs by 16S rDNA gene and FTIR analysis |
| title_full_unstemmed | Identification of novel spp. of rice and wheat endophytic diazotrophs by 16S rDNA gene and FTIR analysis |
| title_short | Identification of novel spp. of rice and wheat endophytic diazotrophs by 16S rDNA gene and FTIR analysis |
| title_sort | identification of novel spp of rice and wheat endophytic diazotrophs by 16s rdna gene and ftir analysis |
| topic | Endophyte Rice Wheat Azospirillum Pseudoxanthomonas Stenotrophomonas |
| url | http://uijs.ui.ac.ir/tbj/browse.php?a_code=A-10-66-2&slc_lang=en&sid=1 |
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