T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures
Aptamers are oligonucleotides that have the characteristic of recognizing a target with high affinity and specificity. Based on our previous studies, the aptamer probe Sgc8-c-Alexa647 is a promising tool for molecular imaging of PTK7, which is an interesting biomarker in cancer. In order to improve...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Pharmaceuticals |
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| Online Access: | https://www.mdpi.com/1424-8247/15/1/15 |
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| author | Romina Castelli Manuel Ibarra Ricardo Faccio Iris Miraballes Marcelo Fernández Albertina Moglioni Pablo Cabral Hugo Cerecetto Romina J. Glisoni Victoria Calzada |
| author_facet | Romina Castelli Manuel Ibarra Ricardo Faccio Iris Miraballes Marcelo Fernández Albertina Moglioni Pablo Cabral Hugo Cerecetto Romina J. Glisoni Victoria Calzada |
| author_sort | Romina Castelli |
| collection | DOAJ |
| description | Aptamers are oligonucleotides that have the characteristic of recognizing a target with high affinity and specificity. Based on our previous studies, the aptamer probe Sgc8-c-Alexa647 is a promising tool for molecular imaging of PTK7, which is an interesting biomarker in cancer. In order to improve the delivery of this probe as well as create a novel drug delivery nanosystem targeted to the PTK7 receptor, we evaluate the co-association between the probe and preformed nanostructures. In this work, preformed pegylated liposomes (PPL) and linear and branched pristine polymeric micelles (PMs), based on PEO–PPO–PEO triblock copolymers were used: poloxamer F127<sup>®</sup> and poloxamines T1307<sup>®</sup> and T908<sup>®</sup>. For it, Sgc8-c-Alexa647 and its co-association with the different nanostructures was exhaustively analyzed. DLS analysis showed nanometric sizes, and TEM and AFM showed notable differences between free- and co-associated probe. Likewise, all nanosystems were evaluated on A20 lymphoma cell line overexpressing PTK7, and the confocal microscopy images showed distinctness in cellular uptake. Finally, the biodistribution in BALB/c mice bearing lymphoma-tumor and pharmacokinetic study revealed an encouraging profile for T908-probe. All data obtained from this work suggested that PMs and, more specifically T908 ones, are good candidates to improve the pharmacokinetics and the tumor uptake of aptamer-based probes. |
| first_indexed | 2024-03-10T00:44:50Z |
| format | Article |
| id | doaj.art-20b69059d7ee4a2cb2257f9852c12d02 |
| institution | Directory Open Access Journal |
| issn | 1424-8247 |
| language | English |
| last_indexed | 2024-03-10T00:44:50Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj.art-20b69059d7ee4a2cb2257f9852c12d022023-11-23T15:00:46ZengMDPI AGPharmaceuticals1424-82472021-12-011511510.3390/ph15010015T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed NanostructuresRomina Castelli0Manuel Ibarra1Ricardo Faccio2Iris Miraballes3Marcelo Fernández4Albertina Moglioni5Pablo Cabral6Hugo Cerecetto7Romina J. Glisoni8Victoria Calzada9Área de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo 11400, UruguayDepartment of Pharmaceutical Sciences, Faculty of Chemistry, Universidad de la República, Montevideo 11800, UruguayÁrea Física, DETEMA, Facultad de Química, Universidad de la República, Montevideo 11800, UruguayInmunología Clínica, (BIOCLIN) y Biotecnología, Polo Tecnológico de Pando-Facultad de Química, Universidad de la República, Montevideo 11800, UruguayLaboratorio de Experimentación Animal, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo 11400, UruguayInstituto IQUIMEFA UBA-CONICET, Departamento de Farmacología, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, CABA, Buenos Aires C1113AAD, ArgentinaÁrea de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo 11400, UruguayÁrea de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo 11400, UruguayInstituto NANOBIOTEC UBA-CONICET, Departamento de Tecnología Farmacéutica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, CABA, Buenos Aires C1113AAD, ArgentinaÁrea de Radiofarmacia, Centro de Investigaciones Nucleares, Facultad de Ciencias, Universidad de la República, Montevideo 11400, UruguayAptamers are oligonucleotides that have the characteristic of recognizing a target with high affinity and specificity. Based on our previous studies, the aptamer probe Sgc8-c-Alexa647 is a promising tool for molecular imaging of PTK7, which is an interesting biomarker in cancer. In order to improve the delivery of this probe as well as create a novel drug delivery nanosystem targeted to the PTK7 receptor, we evaluate the co-association between the probe and preformed nanostructures. In this work, preformed pegylated liposomes (PPL) and linear and branched pristine polymeric micelles (PMs), based on PEO–PPO–PEO triblock copolymers were used: poloxamer F127<sup>®</sup> and poloxamines T1307<sup>®</sup> and T908<sup>®</sup>. For it, Sgc8-c-Alexa647 and its co-association with the different nanostructures was exhaustively analyzed. DLS analysis showed nanometric sizes, and TEM and AFM showed notable differences between free- and co-associated probe. Likewise, all nanosystems were evaluated on A20 lymphoma cell line overexpressing PTK7, and the confocal microscopy images showed distinctness in cellular uptake. Finally, the biodistribution in BALB/c mice bearing lymphoma-tumor and pharmacokinetic study revealed an encouraging profile for T908-probe. All data obtained from this work suggested that PMs and, more specifically T908 ones, are good candidates to improve the pharmacokinetics and the tumor uptake of aptamer-based probes.https://www.mdpi.com/1424-8247/15/1/15Sgc8-c aptamerprobepolymeric micellesliposomesactive targeting |
| spellingShingle | Romina Castelli Manuel Ibarra Ricardo Faccio Iris Miraballes Marcelo Fernández Albertina Moglioni Pablo Cabral Hugo Cerecetto Romina J. Glisoni Victoria Calzada T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures Pharmaceuticals Sgc8-c aptamer probe polymeric micelles liposomes active targeting |
| title | T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures |
| title_full | T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures |
| title_fullStr | T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures |
| title_full_unstemmed | T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures |
| title_short | T908 Polymeric Micelles Improved the Uptake of Sgc8-c Aptamer Probe in Tumor-Bearing Mice: A Co-Association Study between the Probe and Preformed Nanostructures |
| title_sort | t908 polymeric micelles improved the uptake of sgc8 c aptamer probe in tumor bearing mice a co association study between the probe and preformed nanostructures |
| topic | Sgc8-c aptamer probe polymeric micelles liposomes active targeting |
| url | https://www.mdpi.com/1424-8247/15/1/15 |
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