Starvation Promotes Autophagy-Associated Maturation of the Ovary in the Giant Freshwater Prawn, Macrobrachium rosenbergii
Limitation of food availability (starvation) is known to influence the reproductive ability of animals. Autophagy is a lysosomal driven degradation process that protects the cell under metabolic stress conditions, such as during nutrient shortage. Whether, and how starvation-induced autophagy impact...
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Frontiers Media S.A.
2017-05-01
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| Series: | Frontiers in Physiology |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fphys.2017.00300/full |
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| author | Wilairat Kankuan Wilairat Kankuan Chaitip Wanichanon Rossella Titone Attakorn Engsusophon Chanudporn Sumpownon Worawit Suphamungmee Federica Morani Matilde Masini Michela Novelli Ciro Isidoro Prasert Sobhon Prasert Sobhon |
| author_facet | Wilairat Kankuan Wilairat Kankuan Chaitip Wanichanon Rossella Titone Attakorn Engsusophon Chanudporn Sumpownon Worawit Suphamungmee Federica Morani Matilde Masini Michela Novelli Ciro Isidoro Prasert Sobhon Prasert Sobhon |
| author_sort | Wilairat Kankuan |
| collection | DOAJ |
| description | Limitation of food availability (starvation) is known to influence the reproductive ability of animals. Autophagy is a lysosomal driven degradation process that protects the cell under metabolic stress conditions, such as during nutrient shortage. Whether, and how starvation-induced autophagy impacts on the maturation and function of reproductive organs in animals are still open questions. In this study, we have investigated the effects of starvation on histological and cellular changes that may be associated with autophagy in the ovary of the giant freshwater prawn, Macrobachium rosenbergii. To this end, the female prawns were daily fed (controls) or unfed (starvation condition) for up to 12 days, and the ovary tissue was analyzed at different time-points. Starvation triggered ovarian maturation, and concomitantly increased the expression of autophagy markers in vitellogenic oocytes. The immunoreactivities for autophagy markers, including Beclin1, LC3-II, and Lamp1, were enhanced in the late oocytes within the mature ovaries, especially at the vitellogenic stages. These markers co-localized with vitellin in the yolk granules within the oocytes, suggesting that autophagy induced by starvation could drive vitellin utilization, thus promoting ovarian maturation. |
| first_indexed | 2024-12-12T02:55:25Z |
| format | Article |
| id | doaj.art-20b8fb3fd73d47c6919846c635ec08b0 |
| institution | Directory Open Access Journal |
| issn | 1664-042X |
| language | English |
| last_indexed | 2024-12-12T02:55:25Z |
| publishDate | 2017-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Physiology |
| spelling | doaj.art-20b8fb3fd73d47c6919846c635ec08b02022-12-22T00:40:47ZengFrontiers Media S.A.Frontiers in Physiology1664-042X2017-05-01810.3389/fphys.2017.00300264328Starvation Promotes Autophagy-Associated Maturation of the Ovary in the Giant Freshwater Prawn, Macrobrachium rosenbergiiWilairat Kankuan0Wilairat Kankuan1Chaitip Wanichanon2Rossella Titone3Attakorn Engsusophon4Chanudporn Sumpownon5Worawit Suphamungmee6Federica Morani7Matilde Masini8Michela Novelli9Ciro Isidoro10Prasert Sobhon11Prasert Sobhon12Department of Anatomy, Faculty of Science, Mahidol UniversityBangkok, ThailandLaboratory of Molecular Pathology, Department of Health Sciences, Università del Piemonte Orientale “Amedeo Avogadro”Novara, ItalyDepartment of Anatomy, Faculty of Science, Mahidol UniversityBangkok, ThailandLaboratory of Molecular Pathology, Department of Health Sciences, Università del Piemonte Orientale “Amedeo Avogadro”Novara, ItalyDepartment of Anatomy, Faculty of Science, Mahidol UniversityBangkok, ThailandDepartment of Anatomy, Faculty of Science, Mahidol UniversityBangkok, ThailandDepartment of Anatomy, Faculty of Science, Mahidol UniversityBangkok, ThailandLaboratory of Molecular Pathology, Department of Health Sciences, Università del Piemonte Orientale “Amedeo Avogadro”Novara, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of PisaPisa, ItalyDepartment of Translational Research and New Technologies in Medicine and Surgery, University of PisaPisa, ItalyLaboratory of Molecular Pathology, Department of Health Sciences, Università del Piemonte Orientale “Amedeo Avogadro”Novara, ItalyDepartment of Anatomy, Faculty of Science, Mahidol UniversityBangkok, ThailandFaculty of Allied Health Sciences, Burapha UniversityChonburi, ThailandLimitation of food availability (starvation) is known to influence the reproductive ability of animals. Autophagy is a lysosomal driven degradation process that protects the cell under metabolic stress conditions, such as during nutrient shortage. Whether, and how starvation-induced autophagy impacts on the maturation and function of reproductive organs in animals are still open questions. In this study, we have investigated the effects of starvation on histological and cellular changes that may be associated with autophagy in the ovary of the giant freshwater prawn, Macrobachium rosenbergii. To this end, the female prawns were daily fed (controls) or unfed (starvation condition) for up to 12 days, and the ovary tissue was analyzed at different time-points. Starvation triggered ovarian maturation, and concomitantly increased the expression of autophagy markers in vitellogenic oocytes. The immunoreactivities for autophagy markers, including Beclin1, LC3-II, and Lamp1, were enhanced in the late oocytes within the mature ovaries, especially at the vitellogenic stages. These markers co-localized with vitellin in the yolk granules within the oocytes, suggesting that autophagy induced by starvation could drive vitellin utilization, thus promoting ovarian maturation.http://journal.frontiersin.org/article/10.3389/fphys.2017.00300/fullMacrobrachium rosenbergiistarvationautophagyreproductionovarian maturation |
| spellingShingle | Wilairat Kankuan Wilairat Kankuan Chaitip Wanichanon Rossella Titone Attakorn Engsusophon Chanudporn Sumpownon Worawit Suphamungmee Federica Morani Matilde Masini Michela Novelli Ciro Isidoro Prasert Sobhon Prasert Sobhon Starvation Promotes Autophagy-Associated Maturation of the Ovary in the Giant Freshwater Prawn, Macrobrachium rosenbergii Frontiers in Physiology Macrobrachium rosenbergii starvation autophagy reproduction ovarian maturation |
| title | Starvation Promotes Autophagy-Associated Maturation of the Ovary in the Giant Freshwater Prawn, Macrobrachium rosenbergii |
| title_full | Starvation Promotes Autophagy-Associated Maturation of the Ovary in the Giant Freshwater Prawn, Macrobrachium rosenbergii |
| title_fullStr | Starvation Promotes Autophagy-Associated Maturation of the Ovary in the Giant Freshwater Prawn, Macrobrachium rosenbergii |
| title_full_unstemmed | Starvation Promotes Autophagy-Associated Maturation of the Ovary in the Giant Freshwater Prawn, Macrobrachium rosenbergii |
| title_short | Starvation Promotes Autophagy-Associated Maturation of the Ovary in the Giant Freshwater Prawn, Macrobrachium rosenbergii |
| title_sort | starvation promotes autophagy associated maturation of the ovary in the giant freshwater prawn macrobrachium rosenbergii |
| topic | Macrobrachium rosenbergii starvation autophagy reproduction ovarian maturation |
| url | http://journal.frontiersin.org/article/10.3389/fphys.2017.00300/full |
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