Effects of knockout of MT3R/Nqo2 gene on sleep/wake behaviors and EEG power spectrum in rats
Objective To observe the sleep/wake behaviors and EEG power spectrum in melatonin type 3 receptor (MT3R/Nqo2) knockout rats with CRISPR/Cas9 gene editing tool in order to explore the receptor mechanism of melatonin in regulation of sleep/wake behaviors. Methods SD rats with MT3R/Nqo2 knockout were c...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | zho |
| Published: |
Editorial Office of Journal of Army Medical University
2023-05-01
|
| Series: | 陆军军医大学学报 |
| Subjects: | |
| Online Access: | http://aammt.tmmu.edu.cn/html/202212125.htm |
| _version_ | 1797826130514804736 |
|---|---|
| author | LI Xin LI Shiyin LUO Fenlan REN Shuancheng REN Shuancheng |
| author_facet | LI Xin LI Shiyin LUO Fenlan REN Shuancheng REN Shuancheng |
| author_sort | LI Xin |
| collection | DOAJ |
| description | Objective To observe the sleep/wake behaviors and EEG power spectrum in melatonin type 3 receptor (MT3R/Nqo2) knockout rats with CRISPR/Cas9 gene editing tool in order to explore the receptor mechanism of melatonin in regulation of sleep/wake behaviors. Methods SD rats with MT3R/Nqo2 knockout were created by introducing a mutation in the MT3R/Nqo2 gene frame shift by nonhomologous recombination repair causing the gene dysfunction, and the knockout was confirmed at the genetically, transcriptionally, and morphologically by PCR, RT-PCR, and FISH, respectively.Then the changes in sleep/wake behaviors and in EEG power spectrum were observed in the knockout rats and control rats. Results After MT3R/Nqo2 knockout, the knockout rats showed decreased arousal time and increased NREM and REM sleep time during the first 3 h (ZT12~15) after the onset of the dark phase (active phase) when compared with the control rats (P < 0.05).In addition, a significant increase in the number of sleep/wake transitions, a reduction in the duration of wakefulness, and appearance of wake fragmentation were also observed after MT3R/Nqo2 knockout during this period (P < 0.05).EEG power spectrum analysis revealed that NREM sleep θ band energy was elevated in the knockout rats than the control rats (P < 0.05). Conclusion MT3R/Nqo2 knockout results in abnormal power spectrum of EEG especially high-frequency energy during NREM sleep, disrupts sleep homeostasis, leads to decreased arousal, increased sleep, and fragmented arousals in the first 3 h (ZT12~15) after the onset of the dark phase (active phase) in rats.
|
| first_indexed | 2024-03-13T11:04:09Z |
| format | Article |
| id | doaj.art-20b9838775574b5387ffe14fa2c32be2 |
| institution | Directory Open Access Journal |
| issn | 2097-0927 |
| language | zho |
| last_indexed | 2024-03-13T11:04:09Z |
| publishDate | 2023-05-01 |
| publisher | Editorial Office of Journal of Army Medical University |
| record_format | Article |
| series | 陆军军医大学学报 |
| spelling | doaj.art-20b9838775574b5387ffe14fa2c32be22023-05-16T09:14:00ZzhoEditorial Office of Journal of Army Medical University陆军军医大学学报2097-09272023-05-0145993694610.16016/j.2097-0927.202212125Effects of knockout of MT3R/Nqo2 gene on sleep/wake behaviors and EEG power spectrum in ratsLI Xin0 LI Shiyin1LUO Fenlan2REN Shuancheng3REN Shuancheng4Department of Physiology, Collaborative Innovation Center for Brain Science, College of Basic Medical Sciences, Army Medical University (Third Military Medical University), Chongqing, 400038Department of Physiology, Collaborative Innovation Center for Brain Science, College of Basic Medical Sciences, Army Medical University (Third Military Medical University), Chongqing, 400038Department of Physiology, Collaborative Innovation Center for Brain Science, College of Basic Medical Sciences, Army Medical University (Third Military Medical University), Chongqing, 400038Department of Physiology, Collaborative Innovation Center for Brain Science, College of Basic Medical Sciences, Army Medical University (Third Military Medical University), Chongqing, 400038Department of Emergency, No.953 Hospital of PLA Army, Shigatse, Tibet Autonomous Region, 857000, ChinaObjective To observe the sleep/wake behaviors and EEG power spectrum in melatonin type 3 receptor (MT3R/Nqo2) knockout rats with CRISPR/Cas9 gene editing tool in order to explore the receptor mechanism of melatonin in regulation of sleep/wake behaviors. Methods SD rats with MT3R/Nqo2 knockout were created by introducing a mutation in the MT3R/Nqo2 gene frame shift by nonhomologous recombination repair causing the gene dysfunction, and the knockout was confirmed at the genetically, transcriptionally, and morphologically by PCR, RT-PCR, and FISH, respectively.Then the changes in sleep/wake behaviors and in EEG power spectrum were observed in the knockout rats and control rats. Results After MT3R/Nqo2 knockout, the knockout rats showed decreased arousal time and increased NREM and REM sleep time during the first 3 h (ZT12~15) after the onset of the dark phase (active phase) when compared with the control rats (P < 0.05).In addition, a significant increase in the number of sleep/wake transitions, a reduction in the duration of wakefulness, and appearance of wake fragmentation were also observed after MT3R/Nqo2 knockout during this period (P < 0.05).EEG power spectrum analysis revealed that NREM sleep θ band energy was elevated in the knockout rats than the control rats (P < 0.05). Conclusion MT3R/Nqo2 knockout results in abnormal power spectrum of EEG especially high-frequency energy during NREM sleep, disrupts sleep homeostasis, leads to decreased arousal, increased sleep, and fragmented arousals in the first 3 h (ZT12~15) after the onset of the dark phase (active phase) in rats. http://aammt.tmmu.edu.cn/html/202212125.htmmelatoninmt3r/nqo2sleepcrispr/cas9ratssprague-dawleygene knockout |
| spellingShingle | LI Xin LI Shiyin LUO Fenlan REN Shuancheng REN Shuancheng Effects of knockout of MT3R/Nqo2 gene on sleep/wake behaviors and EEG power spectrum in rats 陆军军医大学学报 melatonin mt3r/nqo2 sleep crispr/cas9 rats sprague-dawley gene knockout |
| title | Effects of knockout of MT3R/Nqo2 gene on sleep/wake behaviors and EEG power spectrum in rats |
| title_full | Effects of knockout of MT3R/Nqo2 gene on sleep/wake behaviors and EEG power spectrum in rats |
| title_fullStr | Effects of knockout of MT3R/Nqo2 gene on sleep/wake behaviors and EEG power spectrum in rats |
| title_full_unstemmed | Effects of knockout of MT3R/Nqo2 gene on sleep/wake behaviors and EEG power spectrum in rats |
| title_short | Effects of knockout of MT3R/Nqo2 gene on sleep/wake behaviors and EEG power spectrum in rats |
| title_sort | effects of knockout of mt3r nqo2 gene on sleep wake behaviors and eeg power spectrum in rats |
| topic | melatonin mt3r/nqo2 sleep crispr/cas9 rats sprague-dawley gene knockout |
| url | http://aammt.tmmu.edu.cn/html/202212125.htm |
| work_keys_str_mv | AT lixin effectsofknockoutofmt3rnqo2geneonsleepwakebehaviorsandeegpowerspectruminrats AT lishiyin effectsofknockoutofmt3rnqo2geneonsleepwakebehaviorsandeegpowerspectruminrats AT luofenlan effectsofknockoutofmt3rnqo2geneonsleepwakebehaviorsandeegpowerspectruminrats AT renshuancheng effectsofknockoutofmt3rnqo2geneonsleepwakebehaviorsandeegpowerspectruminrats AT renshuancheng effectsofknockoutofmt3rnqo2geneonsleepwakebehaviorsandeegpowerspectruminrats |