Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems
ABSTRACT: Cryptosporidiosis is considered an infection with impact on animal health. It has been associated with high morbidity and mortality rates, leading to significant economic losses to the poultry industry. This study investigated the presence of Cryptosporidium spp. in domestic ducks of famil...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Santa Maria
2021-10-01
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| Series: | Ciência Rural |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000500501&tlng=en |
| _version_ | 1818988506183106560 |
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| author | Lidiane Tavares Duarte Berriel Adriana Pittella Sudré Helena Lúcia Carneiro Santos Teresa Cristina Bergamo do Bomfim |
| author_facet | Lidiane Tavares Duarte Berriel Adriana Pittella Sudré Helena Lúcia Carneiro Santos Teresa Cristina Bergamo do Bomfim |
| author_sort | Lidiane Tavares Duarte Berriel |
| collection | DOAJ |
| description | ABSTRACT: Cryptosporidiosis is considered an infection with impact on animal health. It has been associated with high morbidity and mortality rates, leading to significant economic losses to the poultry industry. This study investigated the presence of Cryptosporidium spp. in domestic ducks of family Anatidae (Cairina moschata) from two rustic commercial aviaries located in the city of Rio de Janeiro, Brazil. A total of 315 fecal samples were collected from domestic ducks in two different areas (N=186 in area A and N=129 in area B). The microscopic analysis was conducted using a sugar centrifugal flotation technique for the identification of Cryptosporidium spp. oocysts, followed by PCR/sequencing analyses of the partial sequence of the 18S rDNA gene to determine the Cryptosporidium species. Of the 315 samples collected, only 10 (186/5.38%) from area A were positive for Cryptosporidium. The nucleotide sequence and phylogenetic analyses identified that all samples were identical (100%) and belonged to Cryptosporidium baileyi species, which is closely related to gastric species and of importance in animal health. |
| first_indexed | 2024-12-20T19:23:40Z |
| format | Article |
| id | doaj.art-20ba8ff42153463991ce697f9659f394 |
| institution | Directory Open Access Journal |
| issn | 1678-4596 |
| language | English |
| last_indexed | 2024-12-20T19:23:40Z |
| publishDate | 2021-10-01 |
| publisher | Universidade Federal de Santa Maria |
| record_format | Article |
| series | Ciência Rural |
| spelling | doaj.art-20ba8ff42153463991ce697f9659f3942022-12-21T19:28:55ZengUniversidade Federal de Santa MariaCiência Rural1678-45962021-10-0152510.1590/0103-8478cr20210001Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systemsLidiane Tavares Duarte BerrielAdriana Pittella Sudréhttps://orcid.org/0000-0002-0386-6278Helena Lúcia Carneiro Santoshttps://orcid.org/0000-0002-5240-5662Teresa Cristina Bergamo do Bomfimhttps://orcid.org/0000-0002-6741-0202ABSTRACT: Cryptosporidiosis is considered an infection with impact on animal health. It has been associated with high morbidity and mortality rates, leading to significant economic losses to the poultry industry. This study investigated the presence of Cryptosporidium spp. in domestic ducks of family Anatidae (Cairina moschata) from two rustic commercial aviaries located in the city of Rio de Janeiro, Brazil. A total of 315 fecal samples were collected from domestic ducks in two different areas (N=186 in area A and N=129 in area B). The microscopic analysis was conducted using a sugar centrifugal flotation technique for the identification of Cryptosporidium spp. oocysts, followed by PCR/sequencing analyses of the partial sequence of the 18S rDNA gene to determine the Cryptosporidium species. Of the 315 samples collected, only 10 (186/5.38%) from area A were positive for Cryptosporidium. The nucleotide sequence and phylogenetic analyses identified that all samples were identical (100%) and belonged to Cryptosporidium baileyi species, which is closely related to gastric species and of importance in animal health.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000500501&tlng=enmuscovy ducksprotozoanmolecular analysisCryptosporidium baileyi |
| spellingShingle | Lidiane Tavares Duarte Berriel Adriana Pittella Sudré Helena Lúcia Carneiro Santos Teresa Cristina Bergamo do Bomfim Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems Ciência Rural muscovy ducks protozoan molecular analysis Cryptosporidium baileyi |
| title | Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems |
| title_full | Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems |
| title_fullStr | Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems |
| title_full_unstemmed | Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems |
| title_short | Molecular identification of Cryptosporidium baileyi in Muscovy ducks (Cairina moschata domesticus) in free-range production systems |
| title_sort | molecular identification of cryptosporidium baileyi in muscovy ducks cairina moschata domesticus in free range production systems |
| topic | muscovy ducks protozoan molecular analysis Cryptosporidium baileyi |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782022000500501&tlng=en |
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