Primary culture of intestinal epithelial cells as a potential model for Toxoplasma gondii enteric cycle studies
The primary culture of intestinal epithelial cells from domestic cats is an efficient cellular model to study the enteric cycle of Toxoplasma gondii in a definitive host. The parasite-host cell ratio can be pointed out as a decisive factor that determines the intracellular fate of bradyzoites forms....
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Fundação Oswaldo Cruz (FIOCRUZ)
2009-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000600007 |
| _version_ | 1797715763684966400 |
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| author | Marcos de Assis Moura Maria Regina Reis Amendoeira Helene Santos Barbosa |
| author_facet | Marcos de Assis Moura Maria Regina Reis Amendoeira Helene Santos Barbosa |
| author_sort | Marcos de Assis Moura |
| collection | DOAJ |
| description | The primary culture of intestinal epithelial cells from domestic cats is an efficient cellular model to study the enteric cycle of Toxoplasma gondii in a definitive host. The parasite-host cell ratio can be pointed out as a decisive factor that determines the intracellular fate of bradyzoites forms. The development of the syncytial-like forms of T. gondii was observed using the 1:20 bradyzoite-host cell ratio, resulting in similar forms described in in vivo systems. This alternative study potentially opens up the field for investigation into the molecular aspects of this interaction. This can contribute to the development of new strategies for intervention of a main route by which toxoplasmosis spreads. |
| first_indexed | 2024-03-12T08:11:33Z |
| format | Article |
| id | doaj.art-20bd985ea6024b1fb7205a66897fb5c9 |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T08:11:33Z |
| publishDate | 2009-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-20bd985ea6024b1fb7205a66897fb5c92023-09-02T19:06:55ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80602009-09-01104686286410.1590/S0074-02762009000600007Primary culture of intestinal epithelial cells as a potential model for Toxoplasma gondii enteric cycle studiesMarcos de Assis MouraMaria Regina Reis AmendoeiraHelene Santos BarbosaThe primary culture of intestinal epithelial cells from domestic cats is an efficient cellular model to study the enteric cycle of Toxoplasma gondii in a definitive host. The parasite-host cell ratio can be pointed out as a decisive factor that determines the intracellular fate of bradyzoites forms. The development of the syncytial-like forms of T. gondii was observed using the 1:20 bradyzoite-host cell ratio, resulting in similar forms described in in vivo systems. This alternative study potentially opens up the field for investigation into the molecular aspects of this interaction. This can contribute to the development of new strategies for intervention of a main route by which toxoplasmosis spreads.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000600007Toxoplasma gondiicat enteric cycle of T. gondiiintestinal epithelial cellsprimary culture of enterocytes |
| spellingShingle | Marcos de Assis Moura Maria Regina Reis Amendoeira Helene Santos Barbosa Primary culture of intestinal epithelial cells as a potential model for Toxoplasma gondii enteric cycle studies Memorias do Instituto Oswaldo Cruz Toxoplasma gondii cat enteric cycle of T. gondii intestinal epithelial cells primary culture of enterocytes |
| title | Primary culture of intestinal epithelial cells as a potential model for Toxoplasma gondii enteric cycle studies |
| title_full | Primary culture of intestinal epithelial cells as a potential model for Toxoplasma gondii enteric cycle studies |
| title_fullStr | Primary culture of intestinal epithelial cells as a potential model for Toxoplasma gondii enteric cycle studies |
| title_full_unstemmed | Primary culture of intestinal epithelial cells as a potential model for Toxoplasma gondii enteric cycle studies |
| title_short | Primary culture of intestinal epithelial cells as a potential model for Toxoplasma gondii enteric cycle studies |
| title_sort | primary culture of intestinal epithelial cells as a potential model for toxoplasma gondii enteric cycle studies |
| topic | Toxoplasma gondii cat enteric cycle of T. gondii intestinal epithelial cells primary culture of enterocytes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762009000600007 |
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