Altered Gut Microbiota Composition in Subjects Infected With Clonorchis sinensis
Clonorchiasis is an infectious disease caused by helminths of Clonorchis sinensis (C. sinensis). The adult parasite mainly inhabits the bile duct and gall bladder, and results in various complications to the hepatobiliary system. The amount of bile secreted into the intestine is reduced in cases of...
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02292/full |
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| author | Meng Xu Meng Xu Meng Xu Meng Xu Meng Xu Zhihua Jiang Wen Huang Jianhai Yin Jianhai Yin Jianhai Yin Jianhai Yin Jianhai Yin Shen Ou Yanyan Jiang Yanyan Jiang Yanyan Jiang Yanyan Jiang Yanyan Jiang Liyu Meng Shengkui Cao Shengkui Cao Shengkui Cao Shengkui Cao Shengkui Cao Aiping Yu Aiping Yu Aiping Yu Aiping Yu Aiping Yu Jianping Cao Jianping Cao Jianping Cao Jianping Cao Jianping Cao Yujuan Shen Yujuan Shen Yujuan Shen Yujuan Shen Yujuan Shen |
| author_facet | Meng Xu Meng Xu Meng Xu Meng Xu Meng Xu Zhihua Jiang Wen Huang Jianhai Yin Jianhai Yin Jianhai Yin Jianhai Yin Jianhai Yin Shen Ou Yanyan Jiang Yanyan Jiang Yanyan Jiang Yanyan Jiang Yanyan Jiang Liyu Meng Shengkui Cao Shengkui Cao Shengkui Cao Shengkui Cao Shengkui Cao Aiping Yu Aiping Yu Aiping Yu Aiping Yu Aiping Yu Jianping Cao Jianping Cao Jianping Cao Jianping Cao Jianping Cao Yujuan Shen Yujuan Shen Yujuan Shen Yujuan Shen Yujuan Shen |
| author_sort | Meng Xu |
| collection | DOAJ |
| description | Clonorchiasis is an infectious disease caused by helminths of Clonorchis sinensis (C. sinensis). The adult parasite mainly inhabits the bile duct and gall bladder, and results in various complications to the hepatobiliary system. The amount of bile secreted into the intestine is reduced in cases of C. sinensis infection, which may alter the pH of the gut and decrease the amount of surfactant protein D released from the gallbladder. However, the impact of parasitic infection on the human gut microbiome remains unclear. To this end, we examined the gut microbiota composition in 47 modified Kato–Katz thick smear-positive (egg-positive) volunteers and 42 healthy controls from five rural communities. Subjects were grouped into four sub-populations based on age and infection status. High-throughput 16S rRNA gene sequencing revealed significant changes in alpha diversity between EP1 and EN1. The beta diversity showed alterations between C. sinensis-infected subjects and healthy controls. In C. sinensis infected patients, we found the significant reduction of certain taxa, such as Bacteroides and anti-inflammatory Bifidobacterium (P < 0.05). Bacteroides, a predominant gut bacteria in healthy populations, was negatively correlated with the number of C. sinensis eggs per gram (EPG, r = −0.37, P adjust < 0.01 in 20–60 years old group; r = −0.64, P adjust = 0.04 in the 60+ years old group). What’s more, the reduction in the abundance of Bifidobacterium, a common probiotic, was decreased particularly in the 60 + years old group (r = −0.50, P = 0.04). The abundance of Dorea, a potentially pro-inflammatory microbe, was higher in infected subjects than in healthy individuals (P < 0.05). Variovorax was a unique bacteria that was only detected in infected subjects. These results clearly demonstrate the significant influence of C. sinensis infection on the human gut microbiota and provided new insights into the control, prevention, diagnosis, and clinical study of clonorchiasis through the human gut microbiota. |
| first_indexed | 2024-04-13T12:40:41Z |
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| last_indexed | 2024-04-13T12:40:41Z |
| publishDate | 2018-09-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-20c1c6db01064a5ca0910f5a094edce92022-12-22T02:46:31ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-09-01910.3389/fmicb.2018.02292412907Altered Gut Microbiota Composition in Subjects Infected With Clonorchis sinensisMeng Xu0Meng Xu1Meng Xu2Meng Xu3Meng Xu4Zhihua Jiang5Wen Huang6Jianhai Yin7Jianhai Yin8Jianhai Yin9Jianhai Yin10Jianhai Yin11Shen Ou12Yanyan Jiang13Yanyan Jiang14Yanyan Jiang15Yanyan Jiang16Yanyan Jiang17Liyu Meng18Shengkui Cao19Shengkui Cao20Shengkui Cao21Shengkui Cao22Shengkui Cao23Aiping Yu24Aiping Yu25Aiping Yu26Aiping Yu27Aiping Yu28Jianping Cao29Jianping Cao30Jianping Cao31Jianping Cao32Jianping Cao33Yujuan Shen34Yujuan Shen35Yujuan Shen36Yujuan Shen37Yujuan Shen38National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, ChinaChinese Center for Tropical Diseases Research, Shanghai, ChinaWorld Health Organization Collaborating Centre for Tropical Diseases, Shanghai, ChinaNational Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, ChinaKey Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, ChinaGuangxi Zhuang Autonomous Region Center for Disease Control and Prevention, Nanning, ChinaTengxian Center for Disease Control and Prevention, Tengxian, ChinaNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, ChinaChinese Center for Tropical Diseases Research, Shanghai, ChinaWorld Health Organization Collaborating Centre for Tropical Diseases, Shanghai, ChinaNational Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, ChinaKey Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, ChinaTengxian Center for Disease Control and Prevention, Tengxian, ChinaNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, ChinaChinese Center for Tropical Diseases Research, Shanghai, ChinaWorld Health Organization Collaborating Centre for Tropical Diseases, Shanghai, ChinaNational Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, ChinaKey Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, ChinaTengxian Center for Disease Control and Prevention, Tengxian, ChinaNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, ChinaChinese Center for Tropical Diseases Research, Shanghai, ChinaWorld Health Organization Collaborating Centre for Tropical Diseases, Shanghai, ChinaNational Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, ChinaKey Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, ChinaNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, ChinaChinese Center for Tropical Diseases Research, Shanghai, ChinaWorld Health Organization Collaborating Centre for Tropical Diseases, Shanghai, ChinaNational Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, ChinaKey Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, ChinaNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, ChinaChinese Center for Tropical Diseases Research, Shanghai, ChinaWorld Health Organization Collaborating Centre for Tropical Diseases, Shanghai, ChinaNational Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, ChinaKey Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, ChinaNational Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, Shanghai, ChinaChinese Center for Tropical Diseases Research, Shanghai, ChinaWorld Health Organization Collaborating Centre for Tropical Diseases, Shanghai, ChinaNational Center for International Research on Tropical Diseases, Ministry of Science and Technology, Shanghai, ChinaKey Laboratory of Parasite and Vector Biology, Ministry of Health, Shanghai, ChinaClonorchiasis is an infectious disease caused by helminths of Clonorchis sinensis (C. sinensis). The adult parasite mainly inhabits the bile duct and gall bladder, and results in various complications to the hepatobiliary system. The amount of bile secreted into the intestine is reduced in cases of C. sinensis infection, which may alter the pH of the gut and decrease the amount of surfactant protein D released from the gallbladder. However, the impact of parasitic infection on the human gut microbiome remains unclear. To this end, we examined the gut microbiota composition in 47 modified Kato–Katz thick smear-positive (egg-positive) volunteers and 42 healthy controls from five rural communities. Subjects were grouped into four sub-populations based on age and infection status. High-throughput 16S rRNA gene sequencing revealed significant changes in alpha diversity between EP1 and EN1. The beta diversity showed alterations between C. sinensis-infected subjects and healthy controls. In C. sinensis infected patients, we found the significant reduction of certain taxa, such as Bacteroides and anti-inflammatory Bifidobacterium (P < 0.05). Bacteroides, a predominant gut bacteria in healthy populations, was negatively correlated with the number of C. sinensis eggs per gram (EPG, r = −0.37, P adjust < 0.01 in 20–60 years old group; r = −0.64, P adjust = 0.04 in the 60+ years old group). What’s more, the reduction in the abundance of Bifidobacterium, a common probiotic, was decreased particularly in the 60 + years old group (r = −0.50, P = 0.04). The abundance of Dorea, a potentially pro-inflammatory microbe, was higher in infected subjects than in healthy individuals (P < 0.05). Variovorax was a unique bacteria that was only detected in infected subjects. These results clearly demonstrate the significant influence of C. sinensis infection on the human gut microbiota and provided new insights into the control, prevention, diagnosis, and clinical study of clonorchiasis through the human gut microbiota.https://www.frontiersin.org/article/10.3389/fmicb.2018.02292/fullfecal microbiotamicrobiomeClonorchis sinensis16S rRNA gene sequencingdysbiosis |
| spellingShingle | Meng Xu Meng Xu Meng Xu Meng Xu Meng Xu Zhihua Jiang Wen Huang Jianhai Yin Jianhai Yin Jianhai Yin Jianhai Yin Jianhai Yin Shen Ou Yanyan Jiang Yanyan Jiang Yanyan Jiang Yanyan Jiang Yanyan Jiang Liyu Meng Shengkui Cao Shengkui Cao Shengkui Cao Shengkui Cao Shengkui Cao Aiping Yu Aiping Yu Aiping Yu Aiping Yu Aiping Yu Jianping Cao Jianping Cao Jianping Cao Jianping Cao Jianping Cao Yujuan Shen Yujuan Shen Yujuan Shen Yujuan Shen Yujuan Shen Altered Gut Microbiota Composition in Subjects Infected With Clonorchis sinensis Frontiers in Microbiology fecal microbiota microbiome Clonorchis sinensis 16S rRNA gene sequencing dysbiosis |
| title | Altered Gut Microbiota Composition in Subjects Infected With Clonorchis sinensis |
| title_full | Altered Gut Microbiota Composition in Subjects Infected With Clonorchis sinensis |
| title_fullStr | Altered Gut Microbiota Composition in Subjects Infected With Clonorchis sinensis |
| title_full_unstemmed | Altered Gut Microbiota Composition in Subjects Infected With Clonorchis sinensis |
| title_short | Altered Gut Microbiota Composition in Subjects Infected With Clonorchis sinensis |
| title_sort | altered gut microbiota composition in subjects infected with clonorchis sinensis |
| topic | fecal microbiota microbiome Clonorchis sinensis 16S rRNA gene sequencing dysbiosis |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02292/full |
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