Expression of Vitamin D Receptor (VDR) in Urinary Bladder Carcinoma: Immunohistochemical and Histopathological Study
Background: Bladder cancer is the most common malignancy of the urinary tract. Calcitriol [1,25 (OH)2vitamin D3] has anticancer effects mediated through binding to vitamin D receptor (VDR). The expression of VDR is present in many normal and cancer tissues. But, there is little information about its...
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| Format: | Article |
| Language: | English |
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West Asia Organization for Cancer Prevention
2022-09-01
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| Series: | Asian Pacific Journal of Cancer Biology |
| Subjects: | |
| Online Access: | http://www.waocp.com/journal/index.php/apjcb/article/view/916 |
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| author | Rehab Mohamed Sharaf Basma Mostafa Mahmoud Abdalla Samia Ibrahim El Naggar Wesam Ismail Moustafa |
| author_facet | Rehab Mohamed Sharaf Basma Mostafa Mahmoud Abdalla Samia Ibrahim El Naggar Wesam Ismail Moustafa |
| author_sort | Rehab Mohamed Sharaf |
| collection | DOAJ |
| description | Background: Bladder cancer is the most common malignancy of the urinary tract. Calcitriol [1,25 (OH)2vitamin D3] has anticancer effects mediated through binding to vitamin D receptor (VDR). The expression of VDR is present in many normal and cancer tissues. But, there is little information about its expression in urinary bladder carcinoma. This study aimed to analyze VDR immunohistochemical expression in 74 Egyptian patients with urinary bladder carcinoma and to evaluate its association with different clinicopathological parameters.
Methods: Sections from formalin-fixed, paraffin-embedded tumor blocks were stained immunohistochemically using monoclonal anti-VDR antibody. VDR protein expression as well as its immunostaining patterns were recorded and scored separately in each case using semi-quantitative immunoreactive score.
Results: VDR was consistently expressed in the included histologically normal urothelium while tumor cells showed variable degrees of expression. Cytoplasmic/membranous VDR expression was common among the studied cases especially those with urothelial morphology (p = 0.076). While, the mean nuclear VDR was significantly (p = 0.007) higher in non-urothelial tumors. Nuclear VDR was significantly associated with muscle invasion (p = 0.000) and tumor stage (p = 0.001) in urothelial carcinoma. It was also statistically related to tumor grade, stage and muscle invasion in non-urothelial tumors (p = 0.002, 0.003 and 0.012, respectively).
Conclusion: there was a significant relation between nuclear VDR expression and prognostic markers suggesting its decrease as an indicator of a poorer prognosis. Vitamin D supplementation may represent a new treatment option for patients with bladder cancer. |
| first_indexed | 2024-03-08T09:35:31Z |
| format | Article |
| id | doaj.art-20c27b83724243f089219824ac29115e |
| institution | Directory Open Access Journal |
| issn | 2538-4635 |
| language | English |
| last_indexed | 2024-03-08T09:35:31Z |
| publishDate | 2022-09-01 |
| publisher | West Asia Organization for Cancer Prevention |
| record_format | Article |
| series | Asian Pacific Journal of Cancer Biology |
| spelling | doaj.art-20c27b83724243f089219824ac29115e2024-01-30T09:28:52ZengWest Asia Organization for Cancer PreventionAsian Pacific Journal of Cancer Biology2538-46352022-09-017324525310.31557/apjcb.2022.7.3.245-253916Expression of Vitamin D Receptor (VDR) in Urinary Bladder Carcinoma: Immunohistochemical and Histopathological StudyRehab Mohamed Sharaf0Basma Mostafa Mahmoud Abdalla1Samia Ibrahim El Naggar2Wesam Ismail Moustafa3Department of Pathology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.Department of Pathology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.Department of Pathology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.Department of Pathology, Faculty of Medicine, Beni-Suef University, Beni-Suef, Egypt.Background: Bladder cancer is the most common malignancy of the urinary tract. Calcitriol [1,25 (OH)2vitamin D3] has anticancer effects mediated through binding to vitamin D receptor (VDR). The expression of VDR is present in many normal and cancer tissues. But, there is little information about its expression in urinary bladder carcinoma. This study aimed to analyze VDR immunohistochemical expression in 74 Egyptian patients with urinary bladder carcinoma and to evaluate its association with different clinicopathological parameters. Methods: Sections from formalin-fixed, paraffin-embedded tumor blocks were stained immunohistochemically using monoclonal anti-VDR antibody. VDR protein expression as well as its immunostaining patterns were recorded and scored separately in each case using semi-quantitative immunoreactive score. Results: VDR was consistently expressed in the included histologically normal urothelium while tumor cells showed variable degrees of expression. Cytoplasmic/membranous VDR expression was common among the studied cases especially those with urothelial morphology (p = 0.076). While, the mean nuclear VDR was significantly (p = 0.007) higher in non-urothelial tumors. Nuclear VDR was significantly associated with muscle invasion (p = 0.000) and tumor stage (p = 0.001) in urothelial carcinoma. It was also statistically related to tumor grade, stage and muscle invasion in non-urothelial tumors (p = 0.002, 0.003 and 0.012, respectively). Conclusion: there was a significant relation between nuclear VDR expression and prognostic markers suggesting its decrease as an indicator of a poorer prognosis. Vitamin D supplementation may represent a new treatment option for patients with bladder cancer.http://www.waocp.com/journal/index.php/apjcb/article/view/916vdr, immunohistochemistry, urinary bladder, urothelial carcinoma, non-urothelial carcinoma |
| spellingShingle | Rehab Mohamed Sharaf Basma Mostafa Mahmoud Abdalla Samia Ibrahim El Naggar Wesam Ismail Moustafa Expression of Vitamin D Receptor (VDR) in Urinary Bladder Carcinoma: Immunohistochemical and Histopathological Study Asian Pacific Journal of Cancer Biology vdr, immunohistochemistry, urinary bladder, urothelial carcinoma, non-urothelial carcinoma |
| title | Expression of Vitamin D Receptor (VDR) in Urinary Bladder Carcinoma: Immunohistochemical and Histopathological Study |
| title_full | Expression of Vitamin D Receptor (VDR) in Urinary Bladder Carcinoma: Immunohistochemical and Histopathological Study |
| title_fullStr | Expression of Vitamin D Receptor (VDR) in Urinary Bladder Carcinoma: Immunohistochemical and Histopathological Study |
| title_full_unstemmed | Expression of Vitamin D Receptor (VDR) in Urinary Bladder Carcinoma: Immunohistochemical and Histopathological Study |
| title_short | Expression of Vitamin D Receptor (VDR) in Urinary Bladder Carcinoma: Immunohistochemical and Histopathological Study |
| title_sort | expression of vitamin d receptor vdr in urinary bladder carcinoma immunohistochemical and histopathological study |
| topic | vdr, immunohistochemistry, urinary bladder, urothelial carcinoma, non-urothelial carcinoma |
| url | http://www.waocp.com/journal/index.php/apjcb/article/view/916 |
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