Designing Polymerase Chain Reaction for Molecular Diagnostics of the Primary Bacterial Cause of Periodontitis
Background & Objective: Periodontal disease, which can become a chronic condition, is an inflammatory disease that upsets the soft and hard structures supporting the teeth. The aim of the present study was to design and develop an in-house PCR Method, to detect putative periodontitis-related bac...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Fasa University of Medical Sciences
2020-08-01
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| Series: | Journal of Advanced Biomedical Sciences |
| Subjects: | |
| Online Access: | http://jabs.fums.ac.ir/article-1-2097-en.pdf |
| _version_ | 1827812265137537024 |
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| author | mohammad soleimani mohammadreza zolfaghari abbas morovvati |
| author_facet | mohammad soleimani mohammadreza zolfaghari abbas morovvati |
| author_sort | mohammad soleimani |
| collection | DOAJ |
| description | Background & Objective: Periodontal disease, which can become a chronic condition, is an inflammatory disease that upsets the soft and hard structures supporting the teeth. The aim of the present study was to design and develop an in-house PCR Method, to detect putative periodontitis-related bacterial pathogens.
Materials & Methods: The PCR method was launched using specific primers of the five bacteria including Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Tannerella forsythia, Porphyromonas gingivalis and Treponema denticola. Then, the sensitivity and specificity tests were performed for each bacterium after cloning.
Results: Basic specific Primer: hbp Aggregatibacter actinomycetemcomitans، fimA Porphyromonas gingivalis، gene 16s rRNA Prevotella intermedia 16s rRNA Tannerella forsythensis gene and 16s rRNA Treponema denticola 161 bp، 162 bp, 282 bp, 280 bp,173 bp and the sensitivity and specificity tests were performed for this gene.
Conclusion: In order to evaluate and diagnose periodontal diseases using PCR technique, these factors can be identified with high specificity and sensitivity. |
| first_indexed | 2024-03-11T23:16:17Z |
| format | Article |
| id | doaj.art-20c3d5b683c840a3a855d6e2deb2939b |
| institution | Directory Open Access Journal |
| issn | 2228-5105 2783-1523 |
| language | English |
| last_indexed | 2024-03-11T23:16:17Z |
| publishDate | 2020-08-01 |
| publisher | Fasa University of Medical Sciences |
| record_format | Article |
| series | Journal of Advanced Biomedical Sciences |
| spelling | doaj.art-20c3d5b683c840a3a855d6e2deb2939b2023-09-20T21:27:39ZengFasa University of Medical SciencesJournal of Advanced Biomedical Sciences2228-51052783-15232020-08-0110325672577Designing Polymerase Chain Reaction for Molecular Diagnostics of the Primary Bacterial Cause of Periodontitismohammad soleimani0mohammadreza zolfaghari1abbas morovvati2 Department of Microbiology, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran Department of Microbiology, Faculty of Basic Sciences, Qom Branch, Islamic Azad University, Qom, Iran Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran Background & Objective: Periodontal disease, which can become a chronic condition, is an inflammatory disease that upsets the soft and hard structures supporting the teeth. The aim of the present study was to design and develop an in-house PCR Method, to detect putative periodontitis-related bacterial pathogens. Materials & Methods: The PCR method was launched using specific primers of the five bacteria including Aggregatibacter actinomycetemcomitans, Prevotella intermedia, Tannerella forsythia, Porphyromonas gingivalis and Treponema denticola. Then, the sensitivity and specificity tests were performed for each bacterium after cloning. Results: Basic specific Primer: hbp Aggregatibacter actinomycetemcomitans، fimA Porphyromonas gingivalis، gene 16s rRNA Prevotella intermedia 16s rRNA Tannerella forsythensis gene and 16s rRNA Treponema denticola 161 bp، 162 bp, 282 bp, 280 bp,173 bp and the sensitivity and specificity tests were performed for this gene. Conclusion: In order to evaluate and diagnose periodontal diseases using PCR technique, these factors can be identified with high specificity and sensitivity.http://jabs.fums.ac.ir/article-1-2097-en.pdfpolymerase chain reactionperiodontalmolecular diagnostics |
| spellingShingle | mohammad soleimani mohammadreza zolfaghari abbas morovvati Designing Polymerase Chain Reaction for Molecular Diagnostics of the Primary Bacterial Cause of Periodontitis Journal of Advanced Biomedical Sciences polymerase chain reaction periodontal molecular diagnostics |
| title | Designing Polymerase Chain Reaction for Molecular Diagnostics of the Primary Bacterial Cause of Periodontitis |
| title_full | Designing Polymerase Chain Reaction for Molecular Diagnostics of the Primary Bacterial Cause of Periodontitis |
| title_fullStr | Designing Polymerase Chain Reaction for Molecular Diagnostics of the Primary Bacterial Cause of Periodontitis |
| title_full_unstemmed | Designing Polymerase Chain Reaction for Molecular Diagnostics of the Primary Bacterial Cause of Periodontitis |
| title_short | Designing Polymerase Chain Reaction for Molecular Diagnostics of the Primary Bacterial Cause of Periodontitis |
| title_sort | designing polymerase chain reaction for molecular diagnostics of the primary bacterial cause of periodontitis |
| topic | polymerase chain reaction periodontal molecular diagnostics |
| url | http://jabs.fums.ac.ir/article-1-2097-en.pdf |
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