Prevalence and characterisation of methicillin-resistant staphylococci from bovine bulk tank milk in England and Wales
Objectives: To investigate the prevalence and characteristics of methicillin-resistant staphylococci on dairy farms in England and Wales including zoonotic MRSA. Methods: Bulk tank milk was sampled from 363 dairy farms in 2015-2016 and methicillin-resistant staphylococci were isolated by salt broth...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221371652030014X |
| _version_ | 1819042277092229120 |
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| author | Elizabeth A. Fisher Gavin K Paterson |
| author_facet | Elizabeth A. Fisher Gavin K Paterson |
| author_sort | Elizabeth A. Fisher |
| collection | DOAJ |
| description | Objectives: To investigate the prevalence and characteristics of methicillin-resistant staphylococci on dairy farms in England and Wales including zoonotic MRSA. Methods: Bulk tank milk was sampled from 363 dairy farms in 2015-2016 and methicillin-resistant staphylococci were isolated by salt broth enrichment and plating on MRSA Brilliance selective agar. Isolates were characterised through antimicrobial susceptibility testing and whole-genome sequencing. Results: Methicillin-resistant staphylococci were isolated from ∼5% of dairy farms and belonged to six different species, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus lentus, Staphylococcus saprophyticus, Staphylococcus fleurettii and Staphylococcus sciuri. Whole-genome sequencing revealed a large variety of antimicrobial resistance genes and SCCmec elements were present, including mecA and mecC alleles. Potentially zoonotic methicillin-resistance S. aureus were found at a low prevalence (0.83% of sampled dairy farms). Whole-genome sequencing also provided evidence for the mobility of a primordial mec gene complex, independently of a SCCmec element, which appears to have been acquired by S. saprophyticus from S. fleurettii. Conclusions: These data give new insight into the epidemiology of veterinary methicillin-resistant staphylococci to inform future surveillance and zoonotic risk evaluation. Our data indicate that MRSA has likely decreased in prevalence since earlier survey work in England and Wales during 2011-12 and highlights the diversity of methicillin resistance and other resistance determinants among bovine-associated staphylococci with implications for veterinary and human medicine. |
| first_indexed | 2024-12-21T09:38:20Z |
| format | Article |
| id | doaj.art-20c4f23e80784004886878eada377581 |
| institution | Directory Open Access Journal |
| issn | 2213-7165 |
| language | English |
| last_indexed | 2024-12-21T09:38:20Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj.art-20c4f23e80784004886878eada3775812022-12-21T19:08:32ZengElsevierJournal of Global Antimicrobial Resistance2213-71652020-09-0122139144Prevalence and characterisation of methicillin-resistant staphylococci from bovine bulk tank milk in England and WalesElizabeth A. Fisher0Gavin K Paterson1School of Life Sciences, University of Hull, Hull, HU6 7RX, UKEaster Bush Pathology, Royal (Dick) School of Veterinary Studies, UK; The Roslin Institute, University of Edinburgh, Easter Bush Campus, Midlothian, EH25 9RG, UK; Corresponding author.Objectives: To investigate the prevalence and characteristics of methicillin-resistant staphylococci on dairy farms in England and Wales including zoonotic MRSA. Methods: Bulk tank milk was sampled from 363 dairy farms in 2015-2016 and methicillin-resistant staphylococci were isolated by salt broth enrichment and plating on MRSA Brilliance selective agar. Isolates were characterised through antimicrobial susceptibility testing and whole-genome sequencing. Results: Methicillin-resistant staphylococci were isolated from ∼5% of dairy farms and belonged to six different species, Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus lentus, Staphylococcus saprophyticus, Staphylococcus fleurettii and Staphylococcus sciuri. Whole-genome sequencing revealed a large variety of antimicrobial resistance genes and SCCmec elements were present, including mecA and mecC alleles. Potentially zoonotic methicillin-resistance S. aureus were found at a low prevalence (0.83% of sampled dairy farms). Whole-genome sequencing also provided evidence for the mobility of a primordial mec gene complex, independently of a SCCmec element, which appears to have been acquired by S. saprophyticus from S. fleurettii. Conclusions: These data give new insight into the epidemiology of veterinary methicillin-resistant staphylococci to inform future surveillance and zoonotic risk evaluation. Our data indicate that MRSA has likely decreased in prevalence since earlier survey work in England and Wales during 2011-12 and highlights the diversity of methicillin resistance and other resistance determinants among bovine-associated staphylococci with implications for veterinary and human medicine.http://www.sciencedirect.com/science/article/pii/S221371652030014Xstaphylococcizoonosismethicillin-resistanceSCCmecmec genesdairy cattle |
| spellingShingle | Elizabeth A. Fisher Gavin K Paterson Prevalence and characterisation of methicillin-resistant staphylococci from bovine bulk tank milk in England and Wales Journal of Global Antimicrobial Resistance staphylococci zoonosis methicillin-resistance SCCmec mec genes dairy cattle |
| title | Prevalence and characterisation of methicillin-resistant staphylococci from bovine bulk tank milk in England and Wales |
| title_full | Prevalence and characterisation of methicillin-resistant staphylococci from bovine bulk tank milk in England and Wales |
| title_fullStr | Prevalence and characterisation of methicillin-resistant staphylococci from bovine bulk tank milk in England and Wales |
| title_full_unstemmed | Prevalence and characterisation of methicillin-resistant staphylococci from bovine bulk tank milk in England and Wales |
| title_short | Prevalence and characterisation of methicillin-resistant staphylococci from bovine bulk tank milk in England and Wales |
| title_sort | prevalence and characterisation of methicillin resistant staphylococci from bovine bulk tank milk in england and wales |
| topic | staphylococci zoonosis methicillin-resistance SCCmec mec genes dairy cattle |
| url | http://www.sciencedirect.com/science/article/pii/S221371652030014X |
| work_keys_str_mv | AT elizabethafisher prevalenceandcharacterisationofmethicillinresistantstaphylococcifrombovinebulktankmilkinenglandandwales AT gavinkpaterson prevalenceandcharacterisationofmethicillinresistantstaphylococcifrombovinebulktankmilkinenglandandwales |